Human Bone Marrow Stromal Cells Hamper Specific Interactions of CD4 and CD8 T Lymphocytes with Antigen-Presenting Cells

Pira, Giuseppina Li; Ivaldi, Federico; Bottone, Laura; Quarto, Rodolfo; Manca, Fabrizio

doi:10.1016/j.humimm.2006.08.298

Cited by 15 publications

(11 citation statements)

References 36 publications

(53 reference statements)

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“…In contrast, even if BM-MSC after 3 culture passages were also able to inhibit T cell proliferation, CM derived from the culture of these cells was not able to reduce T cell proliferation (Fig. 1B), in accordance to previously reported findings [38], [39].…”

Section: Resultssupporting

confidence: 92%

“…Importantly, hAMTC and hAM were not stimulated prior to CM collection. This is in contrast to MSC derived from BM [38], [39], which possess an anti-proliferative ability only when cultured in the presence of activating stimuli, such as IL-1β, TNF-α or IFN-γ [14]. This underlines the functional differences when using MSC from different sources, most likely due to the heterogeneity of the MSC populations.…”

Section: Discussionmentioning

confidence: 90%

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Characterization of the Conditioned Medium from Amniotic Membrane Cells: Prostaglandins as Key Effectors of Its Immunomodulatory Activity

et al. 2012

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We previously demonstrated that cells isolated from the mesenchymal region of the human amniotic membrane (human amniotic mesenchymal tissue cells, hAMTC) possess immunoregulatory roles, such as inhibition of lymphocyte proliferation and cytokine production, and suppression of generation and maturation of monocyte-derived dendritic cells, as reported for MSC from other sources. The precise factors and mechanisms responsible for the immunoregulatory roles of hAMTC remain unknown. In this study, we aimed to identify the soluble factors released by hAMTC and responsible for the anti-proliferative effect on lymphocytes, and the mechanisms underlying their actions, in vitro. Conditioned medium (CM) was prepared under routine culture conditions from hAMTC (CM-hAMTC) and also from fragments of the whole human amniotic membrane (CM-hAM). We analyzed the thermostability, chemical nature, and the molecular weight of the factors likely responsible for the anti-proliferative effects. We also evaluated the participation of cytokines known to be involved in the immunomodulatory actions of MSC from other sources, and attempted to block different synthetic pathways. We demonstrate that the inhibitory factors are temperature-stable, have a small molecular weight, and are likely of a non-proteinaceous nature. Only inhibition of cyclooxygenase pathway partially reverted the anti-proliferative effect, suggesting prostaglandins as key effector molecules. Factors previously documented to take part in the inhibitory effects of MSCs from other sources (HGF, TGF-β, NO and IDO) were not involved. Furthermore, we prove for the first time that the anti-proliferative effect is intrinsic to the amniotic membrane and cells derived thereof, since it is manifested in the absence of stimulating culture conditions, as opposed to MSC derived from the bone marrow, which possess an anti-proliferative ability only when cultured in the presence of activating stimuli. Finally, we show that the amniotic membrane could be an interesting source of soluble factors, without referring to extensive cell preparation.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 90%

Characterization of the Conditioned Medium from Amniotic Membrane Cells: Prostaglandins as Key Effectors of Its Immunomodulatory Activity

et al. 2012

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“…While the population of pericytes in adipose tissue is large, not all of them are adipose stem cells (Kahn, 2008; Peng and Zhou, 2009; Planat‐Benard et al, 2004). It is likely that only a limited number of pericytes are adipose stem cells (Zannettino et al, 2008; Zimmerlin et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Stem cells are self‐renewing, have long‐term cell viability, and possess multilineage differentiation potential, all of which make them advantageous as seeding cells for regenerative medicine (Langer and Vacanti, 1993; Peng and Zhou, 2009; Reyes et al, 2001). Although embryonic stem cells have multipotent differentiation ability, their use has been restricted owing to ethical and potential immunorejective problems.…”

Section: Introductionmentioning

confidence: 99%

Adipose stem cells originate from perivascular cells

Cai

Lin

Hauschka

et al. 2011

Biology of the Cell

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Recent research has shown that adipose tissues contain abundant MSCs (mesenchymal stem cells). The origin and location of the adipose stem cells, however, remain unknown, presenting an obstacle to the further purification and study of these cells. In the present study, we aimed at investigating the origins of adipose stem cells. α‐SMA (α‐smooth muscle actin) is one of the markers of pericytes. We harvested ASCs (adipose stromal cells) from α‐SMA‐GFP (green fluorescent protein) transgenic mice and sorted them into GFP‐positive and GFP‐negative cells by FACS. Multilineage differentiation tests were applied to examine the pluripotent ability of the α‐SMA‐GFP‐positive and ‐negative cells. Immunofluorescent staining for α‐SMA and PDGF‐Rβ (platelet‐derived growth factor receptor β) were applied to identify the α‐SMA‐GFP‐positive cells. Then α‐SMA‐GFP‐positive cells were loaded on a collagen—fibronectin gel with endothelial cells to test their vascularization ability both in vitro and in vivo. Results show that, in adipose tissue, all of the α‐SMA‐GFP‐positive cells congregate around the blood vessels. Only the α‐SMA‐GFP‐positive cells have multilineage differentiation ability, while the α‐SMA‐GFP‐negative cells can only differentiate in an adipogenic direction. The α‐SMA‐GFP‐positive cells maintained expression of α‐SMA during multilineage differentiation. The α‐SMA‐GFP‐positive cells can promote the vascularization of endothelial cells in three‐dimensional culture both in vitro and in vivo. We conclude that the adipose stem cells originate from perivascular cells and congregate around blood vessels.

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“…8 and 10). It is indicated that the ability of MSCs to interact with the host lymphocytes may be also attributed to their direct contact 30–32. In hydrogels, due to the restriction of the spatial matrix, the seeded cells had limited probability to contact with outside lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Modulation of immunological properties of allogeneic mesenchymal stem cells by collagen scaffolds in cartilage tissue engineering

Yuan

Guo

et al. 2011

J Biomedical Materials Res

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Influence of the structures of some collagen scaffolds on immunological properties of the seeded allogeneic mesenchymal stem cells (MSCs) was studied in this article. Hydrogels, sponge, and membrane were prepared from type-I collagen. These scaffolds were seeded with neonatal rabbit MSCs and cultured for different periods. Changes of the immunological properties associated with different scaffolds were analyzed and compared. It was found that the expression of major histocompatibility complex (MHC) class I and II molecules on MSCs increased gradually in all scaffolds, but the least increment was recorded in hydrogels. Mixed lymphocyte reactions (MLR) showed that the MSC-hydrogel constructs invoked considerably low allogeneic lymphocytes proliferation. Even in presence of interferon-γ (IFN-γ), the hydrogels with higher concentration gave comparatively lower increment of MHC-II expression and allogeneic lymphocytes proliferation. These results suggest that different scaffold structures may provide different microenvironments and extents of isolation from the host immune system for the seed cells, thereby affecting their immunological properties. Therefore, scaffold structures may modulate the immunological properties of tissue-engineered cartilage with allogeneic cells. Hydrogels, especially which were prepared from higher collagen concentrations, were found to be a promising scaffold structure, from the perspective of avoiding severe immune rejection.

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Human Bone Marrow Stromal Cells Hamper Specific Interactions of CD4 and CD8 T Lymphocytes with Antigen-Presenting Cells

Cited by 15 publications

References 36 publications

Characterization of the Conditioned Medium from Amniotic Membrane Cells: Prostaglandins as Key Effectors of Its Immunomodulatory Activity

Characterization of the Conditioned Medium from Amniotic Membrane Cells: Prostaglandins as Key Effectors of Its Immunomodulatory Activity

Adipose stem cells originate from perivascular cells

Modulation of immunological properties of allogeneic mesenchymal stem cells by collagen scaffolds in cartilage tissue engineering

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