Reclassification of the Genus Pasteurella Trevisan 1887 on the Basis of Deoxyribonucleic Acid Homology, with Proposals for the New Species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa

Inter- and Intrafamilial Similarities of rRNA Cistrons of the Pasteurellaceae

“…In an attempt to examine the internal structure of the family Pasteurellaceae, we carried out a large number of hybridizations in which we used DNAs from 53 strains of true and presumed members of this family, including 25 named species (frequently of uncertain affiliation) and 19 strains of uncertain or unknown taxonomic position, and labeled rRNAs from the type strains of the three type species of the three accepted genera and from four other reference strains belonging to the same family (Table 2 and Fig. 2) (41,47,48). For all these reasons we are fairly certain that the resulting rRNA dendrogram covers the whole range of the genetic diversity of the family.…”

“…Most strains belonging to the family Pasteurellaceae were grown primarily on chocolate agar (tryptic soy agar [Difco Laboratories] which after sterilization was supplemented with 5% [voVvol] sheep blood and was kept at 80°C for 15 min before pouring) in a moist atmosphere containing 5 to 10% carbon dioxide at 36°C. Mass cultures were propagated in Difco Proteose peptone medium (78) supplemented with yeast extract (0.5%, wthol), NAD, hematin, or thiamine monophosphate as previously described (12,47,(53)(54)(55).…”

Characterisation and comparison of Pasteurella multocida isolated from different species in the Czech Republic: capsular PCR typing, ribotyping and dermonecrotoxin production

“…Serotyping and biochemical characterisation have indicated a high heterogeneity within the P. multocida species (Brogden and Packer, 1979;Mutters et al, 1985;Blackall et al, 1997), which was later confirmed by a new-generation of typing system based on molecular techniques. Outer membrane and whole cell proteins were shown to be a suitable target for isolate typing (Dabo et al, 1999a;Davies et al, 2003b).…”

Caracterização genotípica de cepas de Pasteurella multocida proveniente de suínos

“…Esses gêneros compartilham várias características, e alguns microorganismos tem sido reclassificados dentro desses gêneros após estudos de hibridização de ácido desoxirribonucléico e sequenciamento de RNAr 16S. Pasteurella aerogenes, P. trehalosi e P. pneumotropica são mais estreitamente relacionadas aos membros do gênero Actinobacillus do que a outras espécies de Pasteurella (MUTTERS et al, 1985).…”

“…A P. multocida é uma espécie heterogênea subdividida em três subespécies de acordo com os padrões de fermentação de carboidratos (MUTTERS et al, 1985).…”