Characterisation and comparison of Pasteurella multocida isolated from different species in the Czech Republic: capsular PCR typing, ribotyping and dermonecrotoxin production

Abstract

ABSTRACT:The aim of this study was to characterise and compare Pasteurella multocida isolates originating from pigs (n = 43), calves (n = 31), rabbits (n = 27), and to a lesser extent from other hosts (n = 6). A total of 107 P. multocida isolates were obtained from various locations in the Czech Republic. They were analysed by capsular PCR typing and ribotyping, and tested for the production of dermonecrotoxin. Most frequently, serogroup A isolates (n = 74) were found, followed by serogroup D (n = 25) and serogroup F (n = 8) isolates. From a total of fifteen different ribotypes (1-15) generated by restriction endonuclease MspI, four ribotypes (1, 3, 4, and 7) were predominant. The prevalence of predominant ribotypes was different in isolates originating from different hosts. Ribotype 1 was characteristic for rabbit isolates, ribotype 3 was primarily found in pig isolates, and ribotype 7 dominated among calf isolates. Sixteen (mainly porcine) isolates produced dermonecrotoxin but significant correlation among ribotypes and dermonecrotoxin production was not observed.

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

“…multocida isolates and strains, and growth conditions. Twenty seven P. multocida rabbit isolates included in this study (Table 1) have already been characterised by capsular PCR typing and ribotyping, and also determined as dermonecrotoxin negative in our previous works (Jaglic et al, 2004(Jaglic et al, , 2005. One of these isolates was designated as a reference strain J-4103 and is available at the Czech Collection of Animal Pathogenic Microorganisms (CAPM 6431).…”

“…This could have been a consequence of a lower sensitivity of the isolates to the complement and other bactericidal proteins, but also of the neutralisation of free endotoxin by plasma components. The rabbit plasma was therefore tested on its ability to bind and Jaglic et al (2005) b including the reference strain J-4103 which was of PGFE Type VIII Figure 2. Electron microscopy of capsule (a) and fimbriae (b) found in Pasteurella multocida isolates.…”

“…However, the finding of the capsular type F in a rabbit host has been recently reported, too (Jaglic et al, 2004). A new generation of typing systems based on molecular techniques allows a more precise discrimination among rabbit isolates of P. multocida and improves the epizootiological study of the disease (Al Haddawi et al, 1999;Dabo et al, 1999Dabo et al, , 2000El Tayeb et al, 2004;Jaglic et al, 2005).…”

“…This study was aimed at a heterogeneous collection of P. multocida rabbit isolates, among which the clear domination of a single ribotype was found in our previous work (Jaglic et al, 2005). In an effort to achieve better discrimination among the isolates, and to find out if a single clone truly prevails in the rabbit population of the Czech Republic, the same isolates were additionally characterised by pulsed-field gel electrophoresis (PFGE).…”

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

“…In addition to the specific relationships between P. multocida serogroups and particular diseases and species, there is also a dominant geographical distribution of capsular types (Zaglic et al, 2005). However, these relationships have exhibited variations in recent years.…”