A strictly aerobic, Gram-negative, non-pigmented bacterial strain, designated LMEB 39 T , was isolated from a seawater sample collected from the Yangtze River estuary near the East China Sea and was examined physiologically, chemotaxonomically and phylogenetically. The novel isolate was motile by a single polar flagellum and positive for nitrate reduction and decomposition of casein, gelatin, Tween 20 and Tween 80, but negative for indole production. Chemotaxonomic analysis revealed ubiquinone-8 as the predominant respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The major fatty acids were C 16 : 1 v7c/iso-C 15 : 0 2-OH, C 16 : 0 , C 18 : 1 v7c, C 12 : 0 3-OH, C 17 : 1 v8c and C 17 : 0 . The genomic DNA G+C content was 42.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Pseudoalteromonas. Strain LMEB 39 T exhibited the closest phylogenetic affinity to Pseudoalteromonas byunsanensis JCM 12483 T (97.4 % sequence similarity). The DNA-DNA reassociation values between strain LMEB 39 T and P. byunsanensis JCM 12483 T and Pseudoalteromonas undina DSM 6065 T (97.2 % sequence similarity) were 31.7 and 30.3 %, respectively. On the basis of phenotypic and genotypic data, strain LMEB 39 T represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas lipolytica sp. nov. is proposed; the type strain is LMEB 39 T (5CGMCC 1.8499 T 5JCM 15903 T ).The genus Pseudoalteromonas was proposed by Gauthier et al. (1995) and represents a clade of marine bacteria grouped by their phylogenetic relationships based on 16S rRNA gene sequences. Members of the genus Pseudoalteromonas are Gram-negative, strictly aerobic, rod-shaped cells. They are motile by a single polar flagellum and have a chemoheterotrophic metabolism (Bowman & McMeekin, 2005). At the time of writing, the genus Pseudoalteromonas comprises 34 species with validly published names (Gauthier et al., 1995;Ivanova et al., 1996Ivanova et al., , 1998Ivanova et al., , 2000Ivanova et al., , 2001 Ivanova et al., , 2002a Ivanova et al., , b, c, d, 2004Bozal et al., 1997; Euzéby, 1997;Bowman, 1998; Holmström et al., 1998; Sawabe et al., 2000;Venkateswaran & Dohmoto, 2000;Egan et al., 2001;Isnansetyo & Kamei, 2003; Romanenko et al., 2003a, b;Lau et al., 2005;Park et al., 2005;Nam et al., 2007;Al Khudary et al., 2008). Some strains produce yellow, red, violet or melanin-like pigments and have the ability to form antibiotic substances (Bowman, 2007). Here, a novel non-pigmented Pseudoalteromonas strain isolated from the Yangtze River estuary was studied using a polyphasic approach.A seawater sample was collected in October 2007 from the Yangtze River estuary (31u 009 040 N 122u 379 260 E) near the East China Sea at a depth of 21 m (temperature 21 u C, salinity 30.5 %). The sample was stored in darkness until micro-organisms were isolated in July 2008. Approximately 80 ml seawater was plated on halophilic medium (HM) containing 10 % (w/v) NaCl by using a te...