Reclassification of 30 <i>Pasteurellaceae</i> strains isolated from rodents

Boot, R.; Bisgaard, Magne

doi:10.1258/002367795781088342

Cited by 31 publications

(21 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mis-identifications of P. gallinaru m and the reasons behind them have been discussed previously by several authors (Bisgaard, 1993;Boot & Bisgaard, 1995;Bisgaard & Mutters, 1986;Frederiksen & Tønning, 2001), the main reason being lack of clear characters for separation due to a high degree of variation. P. gallinaru m shows a high degree of variation in some 10% of the approximately 80 characters used for species identification (Mutters et al, 2003).…”

Section: Discussionmentioning

confidence: 97%

“…Isolates from dogs have been excluded and classified as Bisgaard taxon 16 (Bisgaard & Mutters, 1986). Isolates of P. gallinarum from guinea pigs also represented misclassified strains (Boot & Bisgaard, 1995). In addition, human isolates classified with P. gallinarum most probably represent misidentified [Haemophilus] aphrophilus (Frederiksen & Tønning, 2001) (square brackets indicate that the species was excluded from Haemophilus according to Mutters et al (1989).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotypic heterogeneity of Pasteurella gallinarum as shown by ribotyping and 16S rRNA sequencing

2002

View full text Add to dashboard Cite

Forty-five strains mainly isolated from chickens in Zimbabwe and Denmark, two pig and three rat isolates all identified as Pasteurella gallinarum by conventional phenotypic tests were characterized by ribotyping, and selected strains were subsequently analysed by 16S rRNA gene sequencing. High genotypic diversity was observed, the number of ribotypes totalling 24. A major group of 47 isolates including the type strain of P. gallinarum clustered at 56% similarity and included 21 ribotypes. Ribotyping showed that some genotypes of P. gallinarum seem to be globally distributed. The three isolates from rodents did not share even a single common ribotype fragment with strains from birds and the pig isolates. Two avian isolates from Denmark and Zimbabwe and the pig strain showed from 97.6 to 99.8% 16S rRNA sequence similarity with the type strain of P. gallinarum and with type strains of Pasteurella volantium and Pasteurella avium. Two rat strains showed 98.6% 16S rRNA gene sequence similarity with each other, but were only related with P. gallinarum at 93% similarity. These isolates showed the highest similarity with [Actinobacillus] muris at 96.4 to 95.0% similarity. We suggest that conventional identification of P. gallinarum consequently should consider the source of isolation to obtain a correct diagnosis, and that isolation from animals other than fowl should be confirmed by genotypic analysis such as 16S rRNA gene sequence comparison.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Genotypic heterogeneity of Pasteurella gallinarum as shown by ribotyping and 16S rRNA sequencing

2002

View full text Add to dashboard Cite

show abstract

“…Mráz et al (1979) Organisms tentatively designated as members of the SPgroup have been reported from guinea pigs and from human blood and faeces (Frederiksen, 1981). Guinea pig isolates classified as belonging to the SP-group or taxon 6, respectively, give clearly different results in both biochemical (Boot & Bisgaard, 1995) and serological tests . Based upon 41 phenotypical characteristics, Kunstýr & Hartmann (1983) investigated the prevalence of members of the family Pasteurellaceae in laboratory animals, including guinea pigs.…”

mentioning

confidence: 97%

“…Subsequently, Boot & Walvoort (1986) concluded that otitis media, from which members of the family Pasteurellaceae also could be demonstrated, must be considered a major disease problem in guinea pigs. The importance of including reference or type strains for comparison and extended phenotypic characterization to avoid misclassification was demonstrated by Boot & Bisgaard (1995). Isolates from guinea pigs previously reported as Pasteurella gallinarum (later reclassified as Avibacterium gallinarum) were reclassified as members of the SP-group, taxon 6 or taxon 25.…”

mentioning

confidence: 99%

Mannheimia caviae sp. nov., isolated from epidemic conjunctivitis and otitis media in guinea pigs

Bojesen

Bisgaard

2011

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

Strains T138021-75 T , Pg19 and Pg20 (taxon 25 of Bisgaard) were isolated from guinea pigs and characterized. Strains T138021-75 T and Pg20 showed identical 16S rRNA gene sequences and were distantly related to the published strain P224 with the highest 16S rRNA similarity of 98.6 %. These two strains showed 97.8 % sequence similarity with the type strain and other strains of Mannheimia glucosida and 97.3 % similarity with the type strain of Mannheimia varigena, but ,97 % similarity with all other type strains of the genus Mannheimia, including Mannheimia haemolytica (96.9 %). Phylogenetic analysis of rpoB gene sequences showed that strain P224 had a distant position (89.9 % gene sequence similarity) compared with the three other strains (T138021-75 T , Pg20 and Pg19), which had identical gene sequences. These three novel strains also shared identical recN gene sequences. Phylogenetic analysis of the recN gene sequences showed a close relationship between the three novel strains and strain P224. The DNA-DNA reassociation value between strain T138021-75 T and P224 was 81.6 % and 40.3 % between strain T138021-75 T and the type strain of M. glucosida. Based on the DNA-DNA reassociation data, strain T138021-75 T belonged to a separate species that was closely related to strain P224.Strain P224 differed from strains T138021-75 T , Pg20 and Pg19 in the following phenotypic characteristics: activity of ornithine carboxylase, hydrolysis of glycosides, and acid formation from maltose, dextrin, melibiose and raffinose, as well as reactions for a-galactosidase and b-xylosidase. Whole genome similarity calculations based on recN gene sequences showed that strains T138021-75 T and P224 were related at the species level (0.932), whereas 16S rRNA and partial rpoB gene sequence comparisons showed a more divergent position of strain P224 compared with the novel strains, including a different host of isolation. The results showed that the three strains of taxon 25 represent a novel species for which the name Mannheimia caviae sp. nov. is proposed. The type strain, T138021-75 T (5CCUG 59995 T 5DSM 23207 T ) was isolated from purulent conjunctivitis in guinea pigs. Previous publications have documented both ubiquinones and demethylmenaquinone to be present in the type strain. The G+C content of the DNA of the type strain has been found to be 41.4 mol% (T m ).Although they have been known for decades, the taxonomy of most taxa of the family Pasteurellaceae that are associated with laboratory rodents has remained unsolved (Bisgaard, 1993;Christensen & Bisgaard, 2008). An unclassified Pasteurella-like organism was reported to be associated with subdermal abscesses in guinea pigs by Stewart & Letscher (1976). Very similar isolates were subsequently reported as the 'SP' group by Frederiksen (1981). Several phenotypically identical Pasteurella-like organisms were also reported by Mannheim et al. (1978) from an outbreak of epidemic conjunctivitis in a large colony of guinea pigs. Although widely distributed among mammals and birds, infe...

show abstract

“…CENTER, Tokyo) and incubated at 37°C for 2 days. Pasteurella pneumotropica was identified according to the method of Boot et al [1] and confirmed with the hyperimmune serum for P. pneumotropica ATCC35149. To confirm contamination with P. pneumotropica in the tumor, we also cultured the tumor stock in the manner described above and isolated P. pneumotropica from it.…”

mentioning

confidence: 99%

Detection and Elimination of Contaminating Microorganisms in Transplantable Tumors and Cell Lines.

et al. 2000

View full text Add to dashboard Cite

Abstract:As a quarantine of biological materials, we tested 96 transplantable tumors and cell lines for contamination with microorganisms in a mouse antibody production (MAP) test, enzymatic assay and microbiological culture. Contamination with lactic dehydrogenase elevating virus (LDV), mycoplasmas and Pasteurella pneumotropica was detected. A considerable difference in the contamination rate was observed between in vivo-and in vitro-propagated tumors. LDV in the tumors could be eliminated by both in vitro subculture and subpassage in nude rats. Mycoplasmas were eliminated by means of the mycoplasmaremoval agent and P. pneumotropica by subpassage in mice. These results suggest that there is still a high risk of contamination in transplantable tumors and emphasizes the importance of adequate microbiological quality control. Key words: Contamination, Microorganisms, Transplantable tumor quarantined 907 human tumor samples by using 105 pairs of sentinel mice kept together with tumor-bearing mice, and found contamination with Staphylococcus aureus, Pseudomonas aeruginosa and mouse hepatitis virus, but as far as we know there is no report on the systematic search for contamination with murine pathogens in rodent tumors in Japan.In this report, we present our studies on the contamination of transplantable tumors and cell lines with murine pathogens and their successful elimination.The specimens tested were transplantable tumors and cell lines originating in various animal species. The tumors, which had been propagated either in vivo or in vitro, were obtained from commercial sources

show abstract

Reclassification of 30 Pasteurellaceae strains isolated from rodents

Cited by 31 publications

References 7 publications

Genotypic heterogeneity of Pasteurella gallinarum as shown by ribotyping and 16S rRNA sequencing

Genotypic heterogeneity of Pasteurella gallinarum as shown by ribotyping and 16S rRNA sequencing

Mannheimia caviae sp. nov., isolated from epidemic conjunctivitis and otitis media in guinea pigs

Detection and Elimination of Contaminating Microorganisms in Transplantable Tumors and Cell Lines.

Contact Info

Product

Resources

About