Reclamation of rhizobacteria newly isolated from black pepper plant roots as potential biocontrol agents of root-knot nematodes

Nguyen, Van Bon; Wang, San-Lang; Nguyen, Thi Hanh; Nguyen, Thi Huyen; Trinh, Thi Huyen Trang; Nong, Thi Thiep; Nguyen, To Uyen; Nguyen, Van Nam; Nguyen, Anh Dzung

doi:10.1007/s11164-019-03970-6

Cited by 20 publications

(23 citation statements)

References 35 publications

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“…The PG-producing bacterial strains, including S. marcescens TKU011 [31], S. marcescens TNU01, S. marcescens TNU02 [32], and S. marcescens CC17 [37], were provided from our previous studies [31,32,37]. The marine byproduct, crab shells, were required from Shin-Ma Frozen Food Co. (I-Lan, Taiwan) and processed into demineralized crab shell powder (de-CSP) by the method presented in detail by Wang and Yeh [38].…”

Section: Methodsmentioning

confidence: 99%

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Nguyen

et al. 2020

Marine Drugs

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This study aimed to establish the culture process for the cost-effective production of prodigiosin (PG) from demineralized crab shell powder (de-CSP), a fishery processing byproduct created via fermentation. Among the tested PG-producing strains, Serratia marcescens TNU02 was demonstrated to be the most active strain. Various ratios of protein/de-CSP were used as the sources of C/N for PG biosynthesis. The PG yield was significantly enhanced when the casein/de-CSP ratio was controlled in the range of 3/7 to 4/6. TNU02 produced PG with a high yield (5100 mg/L) in a 15 L bioreactor system containing 4.5 L of a newly-designed liquid medium containing 1.6% C/N source (protein/de-CSP ratio of 3/7), 0.02% (NH4)2SO4, 0.1% K2HPO4, and an initial pH of 6.15, at 27 °C for 8 h in dark conditions. The red pigment was purified from the culture broth and then quantified as being PG by specific Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and UV spectra analysis. The purified PG demonstrated moderate antioxidant and effective inhibition against four cancerous cell lines. Notably, this study was the first to report on using crab wastes for PG bioproduction with high-level productivity (5100 mg/L) in a large scale (4.5 L per pilot) in a short period of fermentation time (8 h). The salt compositions, including (NH4)2SO4 and K2HPO4, were also a novel finding for the enhancement of PG yield by S. marcescens in this report.

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Section: Methodsmentioning

confidence: 99%

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Nguyen

et al. 2020

Marine Drugs

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“…Serratia marcescens TKU011 was obtained from our previous study [9]. S. marcescens CC17 obtained from the previous study [44], S. marcescens TNU01 and S. marcescens TNU02 were newly isolated from the soils of Buon Ma Thuot City, Vietnam, and identified based on 16S gene sequence in this study. Shrimp shell and squid pens were purchased from Fwu-Sow Industry (Taichung, Taiwan).…”

Section: Methodsmentioning

confidence: 99%

Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

et al. 2019

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Marine chitins (MC) have been utilized for the production of vast array of bioactive products, including chitooligomers, chitinase, chitosanase, antioxidants, anti-NO, and antidiabetic compounds. The aim of this study is the bioprocessing of MC into a potent anticancer compound, prodigiosin (PG), via microbial fermentation. This bioactive compound was produced by Serratia marcescens TKU011 with the highest yield of 4.62 mg/mL at the optimal conditions of liquid medium with initial pH of 5.65–6.15 containing 1% α-chitin, 0.6% casein, 0.05% K2HPO4, and 0.1% CaSO4. Fermentation was kept at 25 °C for 2 d. Notably, α-chitin was newly investigated as the major potential material for PG production via fermentation; the salt CaSO4 was also found to play the key role in the enhancement of PG yield of Serratia marcescens fermentation for the first time. PG was qualified and identified based on specific UV, MALDI-TOF MS analysis. In the biological activity tests, purified PG demonstrated potent anticancer activities against A549, Hep G2, MCF-7, and WiDr with the IC50 values of 0.06, 0.04, 0.04, and 0.2 µg/mL, respectively. Mytomycin C, a commercial anti-cancer compound was also tested for comparison purpose, showing weaker activity with the IC50 values of 0.11, 0.1, 0.14, and 0.15 µg/mL, respectively. As such, purified PG displayed higher 2.75-fold, 1.67-fold, and 3.25-fold efficacy than Mytomycin C against MCF-7, A549, and Hep G2, respectively. The results suggest that marine chitins are valuable sources for production of prodigiosin, a potential candidate for cancer drugs.

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“…Endophytic bacteria belong to a bacterial group living in the tissues of plants, including roots, shoots, leaves, and even fruits. Endophytic bacteria interact with the host in growth promotion, nutrition uptake, pathogenic fungal antagonism, and nematode resistance [1][2][3][4][5][6]. For fungal antagonism, endophytic bacteria can release chitinases, beta glucanases, proteases, chemical compounds, and antibiotics, improving the environmental competitiveness [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

et al. 2020

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In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the antifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings treated with the promising bacteria had the lowest rate of root disease (8.45-11.21%) and lower fatal rate (11.11-15.55%) compared to the control group (24.81% and 24.44%). In addition, the three promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.

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Reclamation of rhizobacteria newly isolated from black pepper plant roots as potential biocontrol agents of root-knot nematodes

Cited by 20 publications

References 35 publications

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

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