Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Ngo, Van; Wang, San-Lang; Nguyen, Van Bon; Doan, Chien Thang; Tran, Thi Ngoc; Tran, Dinh Minh; Tran, Trung Dung; Nguyen, Anh Dzung

doi:10.3390/agronomy10020286

Cited by 21 publications

(15 citation statements)

References 33 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, this strain could inhibit up to 98.8% of Phytophthor growth by producing various enzymes and biochemical compounds; this was confirme by in vivo assays on the black pepper seedlings in the green house. We also carried out phylogenetic analysis of 352 endophytic bacteria isolated from 90 root samples in th farms in the Central Highlands of Vietnam for Phytophthora antagonism under in vitr assay conditions [36]. The results showed that Bacillus velezensis and Bacillus amylolique faciens exhibit pathogenic fungal antagonism and plant growth promotion activities Most studies have reported the results of rhizosphere bacteria and endophytic bacteri on the plant growth promotion and antifungal activity using in vitro or green house-based experiments [8,9,27,28,30,34,36].…”

Section: Methodsmentioning

confidence: 99%

“…We also carried out phylogenetic analysis of 352 endophytic bacteria isolated from 90 root samples in th farms in the Central Highlands of Vietnam for Phytophthora antagonism under in vitr assay conditions [36]. The results showed that Bacillus velezensis and Bacillus amylolique faciens exhibit pathogenic fungal antagonism and plant growth promotion activities Most studies have reported the results of rhizosphere bacteria and endophytic bacteri on the plant growth promotion and antifungal activity using in vitro or green house-based experiments [8,9,27,28,30,34,36]. To date, few works have researched th impact of rhizosphere bacteria and endophytic bacteria on the growth and disease re sistance of black pepper in the field.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

et al. 2020

Self Cite

View full text Add to dashboard Cite

Black pepper (Piper nigrum L.) is one of the most important crops and global demand continues to increase, giving it a high export value. However, black pepper cultivation has been seriously affected by a number of pathogenic diseases. Among them, “quick wilt” caused by Phytophthora sp., “slow decline” caused by Fusarium sp., and root-knot nematode Meloidogyne sp. have a serious negative effect on black pepper growth and productivity. There have been different chemical and biological methods applied to control these diseases, but their effectiveness has been limited. The aim of this research was to evaluate different combinations of rhizosphere bacteria and endophytic bacteria isolated from black pepper farms in the Central Highland of Vietnam for their ability to suppress pathogens and promote black pepper growth and yield. Formula 6, containing the strains Bacillus velezensis KN12, Bacillus amyloliquefaciens DL1, Bacillus velezensis DS29, Bacillus subtilis BH15, Bacillus subtilis V1.21 and Bacillus cereus CS30 exhibited the largest effect against Phytophthora and Fusarium in the soil and in the roots of black pepper. These bio-products also increased chlorophyll a and b contents, which led to a 1.5-fold increase of the photosynthetic intensity than the control formula and a 4.5% increase in the peppercorn yield (3.45 vs. 3.30 tons per hectare for the control). Our results suggest that the application of rhizosphere and endophytic bacteria is a promising method for disease control and growth-promotion of black pepper.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Anti-fungal activity of Pseudomonas aeruginosa TUN03 was assessed according to the method per Ngo et al 2020 [33]. A total of 12 fungal pathogen strains (supported from the Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Vietnam), including Purpureocillium lilacinum F01, Fusarium solani F02, F. solani F03, F. solani F04, Colletotrichum gloeosporioides F05, F. incarnatum F06, Gongronella butleri F07, Pestalotiopsis mangiferae F08, F. incarnatum F09, F. oxysporum F10, Neonectria sp.…”

Section: Anti-fungal Assaysmentioning

confidence: 99%

“…Assessment of in vitro antifungal activity test of Pseudomonas aeruginosa TUN03 against various plant-pathogen fungal strains. : the activity was observed but not clear for harvesting the data based on the method reported by Ngo et al 2020[33]. All the experiments were performed in triplicate.…”

mentioning

confidence: 96%

Potential Application of Rhizobacteria Isolated from the Central Highland of Vietnam as an Effective Biocontrol Agent of Robusta Coffee Nematodes and as a Bio-Fertilizer

et al. 2021

Self Cite

View full text Add to dashboard Cite

Robusta coffee is a major commercial crop in the Central Highland of Vietnam with high economic and export value. However, this crop is adversely affected by various pathogens, particularly nematodes. This study aimed to screen active anti-nematode rhizobacterial strains for sustainable coffee production. Among more than 200 isolates, the isolate TUN03 demonstrated efficient biocontrol with nearly 100% mortality of J2 coffee nematodes Meloidogyne spp. and 84% inhibition of nematode egg hatching. This active strain was identified as Pseudomonas aeruginosa TUN03 based on its 16S rRNA gene sequence and phylogenetic analysis. In greenhouse tests, the strain TUN03 significantly reduced the coffee nematode population in the rhizome-soil with an 83.23% inhibition rate and showed plant growth-promoting effects. Notably, this is the first report of the nematicidal effect of P. aeruginosa against coffee nematodes. This potent strain further showed an antifungal effect against various crop-pathogenic fungi and was found to be the most effective against Fusarium solani F04 (isolated from coffee roots) with a 70.51% inhibition rate. In addition, high-performance liquid chromatography analysis revealed that this bacterial strain also secretes plant growth regulators including indole acetic acid (IAA), gibberellic acid (GA3), kinetin, and zeatin in significant amounts of 100, 2700, 37, and 9.5 µg/mL, respectively. The data from this study suggest that P. aeruginosa TUN03 may be a potential biocontrol agent and biofertilizer for the sustainable production of Robusta coffee and other crops.

show abstract

“…The zoospores were not able to germinate when they were cultured in the presence of this compound. Ngo et al, (2020) [52] isolated endophytic black pepper bacteria, of which six showed the ability to inhibit the growth of Phytophthora spp. by more than 60%.…”

Section: Biocontrol Of Phytophthora Spp By Endophytic Bacteriamentioning

confidence: 99%

Endophytic Microorganisms as an Alternative for the Biocontrol of Phytophthora spp.

Bolívar-Anillo¹,

González‐Rodríguez²,

Almeida³

et al. 2021

Agro-Economic Risks of Phytophthora and an Effective Biocontrol Approach

View full text Add to dashboard Cite

The genus Phytophthora with more than 100 described species and 58 officially recognized, phylogenetically distributed in ten clades, are important pathogenic oomycete chromists that cause important diseases in agricultural crops, trees and forests worldwide. This genus is known as \"The Plant Destroyer” which causes great economic losses with costs between 2 and 7 billion dollars per year in agricultural systems and unquantifiable losses in natural ecosystems. The host plants of the genus Phytophthora can vary from a wide range in some species to only one host, however, the host plants of the new species are still being determined and therefore the range continues to expand, that makes control exceedingly difficult. Plant damage can range from alterations in roots, fruits, trunks, stems, foliage and crown to invasive processes in highly susceptible species. Considering the wide range of hosts and organs that can be affected by Phytophthora, the use of endophytic microorganisms for the biocontrol of this phytopathogen can be an alternative to avoid losses of both crops and forests worldwide. Endophytes are microorganisms that live inside plant tissues without causing disease under any circumstances. The fact that endophytic microorganisms are able to colonize an ecological niche similar to that of some plant pathogens qualifies them as potential biocontrol agents. This chapter describes the endophytic bacteria and fungi isolated from different plant species that have shown antagonistic activity against different species of Phytophthora, as well as the metabolites isolated from these microorganisms that have shown fungicide activity and other biocontrol strategies (enzyme production, siderophores, substrate competition, among others) against Phytophthora.

show abstract

Phytophthora Antagonism of Endophytic Bacteria Isolated from Roots of Black Pepper (Piper nigrum L.)

Cited by 21 publications

References 33 publications

Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

Potential Application of Rhizobacteria Isolated from the Central Highland of Vietnam as an Effective Biocontrol Agent of Robusta Coffee Nematodes and as a Bio-Fertilizer

Endophytic Microorganisms as an Alternative for the Biocontrol of Phytophthora spp.

Contact Info

Product

Resources

About