Reciprocal regulation of miR-1205 and E2F1 modulates progression of laryngeal squamous cell carcinoma

Abstract

The burgeoning functions of many microRNAs (miRs) have been well study in cancer. However, the level and function of miR-1205 in laryngeal squamous cell cancer remains unknown. In the current research, we validated that miR-1205 was notably downregulated in human laryngeal squamous cell carcinoma (LSCC) samples in comparison with tissues adjacent to LSCC, and correlated with T stage, lymph node metastasis, and clinical stage. Using Kaplan–Meier analysis indicates that high expression of miR-1205 has a favorable prognosis for patients with LSCC. Functional assays show that enforced miR-1205 expression attenuates the migration, growth, and invasion of LSCC cells. And E2F1 is verified to be a target of miR-1205, while E2F1 binds to miR-1205 promoter and transcriptionally inhibits miR-1205 expression. Overexpression of E2F1 reverses the inhibitory impacts of miR-1205 on LSCC cells in part. Importantly, E2F1 is abnormally increased in LSCC tissues, and its protein levels were inversely relevant to miR-1205 expression. High E2F1 protein level is in connection with clinical stage, T stage, lymph node metastasis, and poor prognosis. Consequently, reciprocal regulation of miR-1205 and E2F1 plays a crucial role in the progression of LSCC, suggesting a new miR-1205/E2F1-based clinical application for patients of LSCC.

miR-1207-5p suppresses laryngeal squamous cell carcinoma progression by downregulating SKA3 and inhibiting epithelial-mesenchymal transition

“…16,17 By directly decreasing suppressor of cytokine signaling 2 (SOCS2) expression, miR-196b can increase the invasive and proliferative ability of LSCC cells while suppressing apoptosis, 18 whereas high levels of miR-1205 inhibit the growth, migratory, and invasive abilities of LSCC cells. 19 These findings suggest that miRNAs play an important role in the progression of LSCC.…”

“…Understanding the mechanism of LSCC progression, invasion, and metastasis, as well as identifying more reliable biomarkers, is therefore critical for LSCC diagnosis and treatment. miRNAs are non-coding endogenous RNA molecules made up of [18][19][20][21][22] nucleotides that primarily dock the 3 0 untranslated region (UTR) of a target mRNA, causing degeneration or translation suppression. 13 miRNAs have been implicated in the pathogenesis of various tumors, as well as regulating cell proliferation, invasion, migration, chemoresistance, and angiogenesis, via multiple signaling pathways.…”

circCYFIP2 Acts as a Sponge of miR-1205 and Affects the Expression of Its Target Gene E2F1 to Regulate Gastric Cancer Metastasis

“…19 Recently, miR-1205 has been reported as a tumor suppressor that is significantly downregulated in multiple cancer types. 20,21 In our study, E2F1 was predicted as the candidate target gene of miR-1205 by miRDB and Targetscan and was further testified by dual-luciferase reporter assay. Recently, a few studies have implicated that overexpression of E2F1 accelerates formation of various tumors.…”

E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375

“…Moreover, a multiscale integrated analysis revealed that E2F1 showed excellent diagnostic and prognostic values, which may be a potential regulator in esophageal cancer [ 10 ]. Interestingly, E2F1 has been identified to bind to microRNA (miR)-1205 promoter and transcriptionally inhibit miR-1205 expression in laryngeal SCC [ 11 ]. Therefore, we wondered whether E2F1 serves as a transcription factor to repress the transcription levels of miRNAs in ESCC.…”