miR-1207-5p suppresses laryngeal squamous cell carcinoma progression by downregulating SKA3 and inhibiting epithelial-mesenchymal transition

Wu, Yongyan; Dai, Fengsheng; Zhang, Yuliang; Zheng, Xian; Li, Li; Cao, Ji‐Min; Gao, Wei

doi:10.1016/j.omto.2021.08.001

Cited by 8 publications

(8 citation statements)

References 51 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Western blot analysis showed that elevated SKA3 expression is significantly and positively associated with the increased expression of p-Rb, E2F1, CDK4, cyclin D1, CDK2, cyclin E2, and p-Akt in HeLa cells ( 25 ). In addition, SKA3 knockdown inhibited epithelial–mesenchymal transition ( 26 ).…”

Section: Discussionmentioning

confidence: 99%

SKA1/2/3 is a biomarker of poor prognosis in human hepatocellular carcinoma

Song

He²,

Ji³

et al. 2022

Front. Oncol.

View full text Add to dashboard Cite

BackgroundSpindle and kinetochore-associated complex subunits 1–3 (SKA1–3) stabilize the kinetochore-attached spindle microtubules in metaphase. Due to the dysregulation in multiple cancers, SKA1–3 is considered a predictor for the prognosis of the patients. However, the potential clinical applications of SKA1–3, particularly in hepatocellular carcinoma (HCC) prognosis and progression, have completely unknown yet.MethodsFor the analysis of SKA1–3 expression and applications in clinics in HCC patients, several databases, such as STRING, UALCAN, GEO, and TCGA, were searched. In addition, the underlying mechanisms of SKA for the regulation of HCC occurrence, development, and progression were also explored.ResultsCompared to the normal controls, HCC patients showed dramatically elevated SKA1–3 expression at the mRNA level, and the values of the area under the curve (AUC) were 0.982, 0.887, and 0.973, respectively. Increased SKA1–3 expression levels were associated with the clinical stage, age, body mass index, tumor grade, tissue subtype, and Tp53 mutation status in HCC patients. The analyses of Kyoto Encyclopedia of Genes and Genome (KEGG) and Gene ontology (GO) demonstrated that SKA1–3 are enriched mainly in the Fanconi anemia, homologous recombination, spliceosome, DNA replication, and cell cycle signaling pathways. The hub genes, such as CDK1, CCNB1, CCNA2, TOP2A, BUB1, AURKB, CCNB2, BUB1B, NCAPG, and KIF11, were identified in protein–protein interactions (PPIs). The expression levels of hub genes were increased in HCC patients and predictive of a poor prognosis. Finally, the expression levels of SKA1–3 were determined using the GEO database.ConclusionsSKA1–3 are potential prognostic biomarkers of and targets for HCC. In addition, SKA1–3 may affect HCC prognosis via the Fanconi anemia pathway, homologous recombination, spliceosome, DNA replication, and cell cycle signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

SKA1/2/3 is a biomarker of poor prognosis in human hepatocellular carcinoma

Song

He²,

Ji³

et al. 2022

Front. Oncol.

View full text Add to dashboard Cite

show abstract

“…Cell proliferation was assessed using the EdU staining kit (RiboBio, Guangzhou, China) according to the manufacturer’s protocol. Trans-well migration and invasion assays were performed as previously described with modification ( 27 ). Briefly, for the migration assay, cells were seeded in 24-well trans-well plates (Falcon, USA) with a density of 1 × 10 6 cells per well in 100 µL of serum-free media.…”

Section: Methodsmentioning

confidence: 99%

Identification of a seven-lncRNAs panel that serves as a prognosis predictor and contributes to the malignant progression of laryngeal squamous cell carcinoma

et al. 2023

Self Cite

View full text Add to dashboard Cite

BackgroundLaryngeal squamous cell carcinoma (LSCC) is one of the most frequent head and neck cancers worldwide. Long non-coding RNAs (lncRNAs) play a critical role in tumorigenesis. However, the clinical significance of lncRNAs in LSCC remains largely unknown.MethodsIn this study, transcriptome sequencing was performed on 107 LSCC and paired adjacent normal mucosa (ANM) tissues. Furthermore, RNA expression and clinical data of 111 LSCC samples were obtained from The Cancer Genome Atlas (TCGA) database. Bioinformatics analysis were performed to construct a model for predicting the overall survival (OS) of LSCC patients. Moreover, we investigated the roles of lncRNAs in LSCC cells through loss-of-function experiments.ResultsA seven-lncRNAs panel including ENSG00000233397, BARX1-DT, LSAMP-AS1, HOXB-AS4, MNX1-AS1, LINC01385, and LINC02893 was identified. The Kaplan–Meier analysis demonstrated that the seven-lncRNAs panel was significantly associated with OS (HR:6.21 [3.27-11.81], p-value<0.0001), disease-specific survival (DSS) (HR:4.34 [1.83-10.26], p-value=0.0008), and progression-free interval (PFI) (HR:3.78 [1.92-7.43], p-value=0.0001). ROC curves showed the seven-lncRNAs panel predicts OS with good specificity and sensitivity. Separately silencing the seven lncRNAs inhibited the proliferation, migration, and invasion capacity of LSCC cells.ConclusionCollectively, this seven-lncRNAs panel is a promising signature for predicting the prognosis of LSCC patients, and these lncRNAs could serve as potential targets for LSCC treatment.

show abstract

“…5 C). In addition, downregulation of miR-1207-5p was found to be strongly linked to the malignant progression of LSCC while miR-1207-5p upregulation suppressed LSCC cell proliferation, invasion, migration, and xenograft tumor growth 74 . Mechanistic studies revealed that miR-1207-5p primarily inhibited SKA3’s activation of PLK1 and the PI3K/Akt signaling pathway to prevent the growth of cancer cells.…”

Section: Role Of Ska3 In Human Cancermentioning

confidence: 97%

SKA3 targeted therapies in cancer precision surgery: bridging bench discoveries to clinical applications – review article

Feng,

Wang,

Xiao

et al. 2024

International Journal of Surgery

View full text Add to dashboard Cite

Spindle and kinetochore-associated complex subunit 3 (SKA3) is a microtubule-binding subcomplex of the outer kinetochore which plays a vital role in proper chromosomal segregation and cell division. Recently, SKA3 have been demonstrated its oncogenic role of tumorigenesis and development in cancers. In this review, we comprehensively deciphered SKA3 in human cancer from various aspects, including bibliometrics, pan-cancer analysis and narrative summary. We also provided top 10 predicted drugs targeting SKA3. We proposed that SKA3 was a potential target and brought new therapeutic opportunities for cancer patients.

show abstract

miR-1207-5p suppresses laryngeal squamous cell carcinoma progression by downregulating SKA3 and inhibiting epithelial-mesenchymal transition

Cited by 8 publications

References 51 publications

SKA1/2/3 is a biomarker of poor prognosis in human hepatocellular carcinoma

SKA1/2/3 is a biomarker of poor prognosis in human hepatocellular carcinoma

Identification of a seven-lncRNAs panel that serves as a prognosis predictor and contributes to the malignant progression of laryngeal squamous cell carcinoma

SKA3 targeted therapies in cancer precision surgery: bridging bench discoveries to clinical applications – review article

Contact Info

Product

Resources

About