Reciprocal expression of CD38 and CD34 by adult murine hematopoietic stem cells

Abstract

The effects of activation of adult murine stem cells on their expression of CD38 were studied using a murine transplantation model. First, the published finding that the majority of long-term engrafting cells from normal adult steady-state marrow are CD38 ؉ was confirmed. Next, it was determined that the majority of stem

CD38 expression by hematopoietic stem cells of newborn and juvenile mice

“…This direction of changes is opposite to that of the changes seen in adult HSCs. 6 Earlier, Randall et al 7 demonstrated that only CD38 high population, but not CD38 − population, in Sca1 + , c-kit + , Lin low/− liver cells from 14.5 days post coitum (dpc) mice and adult mice are capable of long-term hematopoietic reconstitution. Dagher et al 8 also demonstrated that only CD38 + population in c-kit + , Lin − yolk sac cells derived from 9 dpc mice, as well as adult mice are capable of long-term reconstitution.…”

“…6 To determine whether there are similar effects on stem cells in 5-week-old mice, we tested CD38 expression by HSCs of 5-week-old mice following 5-FU injection. Because the effects of 5-FU injection on the expression of CD34 by HSCs in 5-week-old mice are not known, we separated bone marrow cells on the basis of CD38 expression Leukemia alone.…”

“…2 Regarding stem cell CD38 expression, investigators in four different laboratories including ours reported that HSCs in yolk sac, fetal liver and adult mouse bone marrow express CD38. [6][7][8][9] We demonstrated that activation of adult murine HSCs causes reversible changes in CD38 expression. 6 Unknown has been the state of CD38 expression by HSCs of newborn or juvenile mice.…”

“…[6][7][8][9] We demonstrated that activation of adult murine HSCs causes reversible changes in CD38 expression. 6 Unknown has been the state of CD38 expression by HSCs of newborn or juvenile mice. This question has significant clinical relevance because cord blood cells recently have emerged as an important source for stem cell transplantation.…”

Bone Marrow‐Derived Stem Cells Can Differentiate into Retinal Cells in Injured Rat Retina

“…We prepared partially purified stem cell-enriched BMCs as previously described [26][27][28]. Briefly, 5-fluorouracil (Kyowa Hakko Kogyo; Tokyo, Japan) was injected into the peritoneal cavity of 4-week-old male Brown Norway rats (Clea Japan; Osaka, Japan) at 150 mg/kg body weight 48 hours before sacrifice.…”