CD38 expression by hematopoietic stem cells of newborn and juvenile mice

Higuchi, Yusuke; Zeng, Haiqun; Ogawa, Makio

doi:10.1038/sj.leu.2402785

Cited by 24 publications

(20 citation statements)

References 15 publications

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“…However, we recently observed in our laboratory that neonatal murine stem cells are CD38 À . 17 Therefore, our studies of CD38 expression by human CB stem cells are again in agreement with the studies of murine stem cells.…”

Section: Figuresupporting

confidence: 79%

Human cord blood long-term engrafting cells are CD34+ CD38−

et al. 2003

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There have been controversies about CD34 and CD38 expression by human cord blood (CB) stem cells. Using the newborn NOD/SCID/b2-microglobulin-null mouse assay that we recently developed, we examined the in vivo engrafting capability of human CB cells. Almost all of the 4-5 months engrafting cells were found in CD34 + population. The capability of secondary reconstitution was found only in the CD34 + cells. When the CD34 + CB cells were separated into CD38 À and CD38 + subpopulations and tested for engraftment, the majority of the engrafting cells were detected in the CD38 À subpopulation. These findings are consistent with the results from studies of murine stem cells and strongly indicate that the phenotype of human CB stem cells is CD34 + CD38 À . Leukemia (2003) 17, 960-964.

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Section: Figuresupporting

confidence: 79%

Human cord blood long-term engrafting cells are CD34+ CD38−

et al. 2003

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“…on May 11, 2018. by guest www.bloodjournal.org From appear to parallel those described for AA4.1, CD34, CD38, and Mac1 expression on murine HSCs, 18,19,22 it is inviting to speculate that all of these are mediated by a common pathway that is regulated in HSCs by a cell cycle checkpoint at the G o /G 1 interface.…”

Section: Discussionmentioning

confidence: 95%

“…Thus, when they are activated, expression of these antigens may be either upregulated (eg, CD34 and Mac1) or down-regulated (eg, CD38) without affecting the regenerative potential of the HSCs. 18,19,22 The abilities of HSCs from adult mice to efflux Rhodamine-123 and Hoechst 33342 efficiently are properties that are lost rapidly as these cells begin to differentiate. 23,24 The ability of HSCs to efflux Hoechst 33342 has received particular attention because this property is shared with other primitive cell types, including muscle and cardiac stem cells, 34,35 neural/retinal stem cells, 36,37 and subsets of ES cells, 26 as well as populations of primitive hematopoietic cells from other species, including humans.…”

Section: Discussionmentioning

confidence: 99%

“…[10][11][12][13][14] However, the expression of many of the markers targeted in these purification procedures, including AA4.1, Mac-1, CD34, and CD38, are now known to alter during ontogeny or when adult mouse HSCs are stimulated to proliferate. [15][16][17][18][19][20][21][22] This has made it more difficult to obtain comparably purified populations of HSCs from fetal sources or after various in vitro and in vivo manipulations of adult HSCs. Other properties of HSCs present in steady-state adult mouse bone marrow include a verapamil-sensitive ability to efflux Rhodamine-123 23 and Hoechst 33342.…”

Section: Introductionmentioning

confidence: 99%

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ABC transporter activities of murine hematopoietic stem cells vary according to their developmental and activation status

Uchida

Dykstra

Lyons

et al. 2004

Blood

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“…[15][16][17] It is also reminiscent of the absence of the differentiation marker CD38 on human HSCs 11,[18][19][20][21] and its presence on mouse HSCs. [22][23][24][25][26] Additional transplantation studies will be useful to further characterize the repopulating potential of the CD150 Ϫ CD48 ϩ population in large mammals.…”

Section: Resultsmentioning

confidence: 99%

Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markers

Larochelle

Savona

Wiggins

et al. 2011

Blood

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Various combinations of antibodies directed to cell surface markers have been used to isolate human and rhesus macaque hematopoietic stem cells (HSCs). These protocols result in poor enrichment or require multiple complex steps. Recently, a simple phenotype for HSCs based on cell surface markers from the signaling lymphocyte activation molecule (SLAM) family of receptors has been reported in the mouse. We examined the possibility of using the SLAM IntroductionThe search for hematopoietic stem cell (HSC)-specific surface markers has been a central question for functional stem cell studies and for the development of clinical applications, including transplantation and gene therapy. In mice, using a complex combination of positive and negative selection for 10 to 12 surface markers (Lin Ϫ Sca-1 ϩ c-kit ϩ Thy-1 lo ), 1 in 4.9 cells provides long-term reconstitution after intravenous injection. 1 However, the use of these complex sets of markers, alone or in combination with the established isolation scheme based on Hoechst dye efflux (side population), 2 is incompatible with in situ histologic analyses.Recently, a simple and broadly applicable method to isolate mouse HSCs has been developed based on expression of cell surface markers, which are members of the signaling lymphocyte activation molecule (SLAM) family, including CD150, CD48, and CD244. 1 In transplantation assays, 1 in 4.8 CD150 ϩ CD48 Ϫ bone marrow (BM) cells provides longterm, multilineage reconstitution in recipient mice, similar to the enrichment obtained in the complex Lin Ϫ Sca-1 ϩ c-kit ϩ Thy-1 lo population. 1 This same strategy has been successfully applied to enrich HSCs from mouse cyclophosphamide/granulocytecolony stimulating factor (G-CSF)-mobilized cells, 3 mouse fetal liver, 4 as well as from the BM of various strains of mice 5 and older mice. 3 Recent data examining the overlap between SLAM family member expression with the Hoechst dye efflux side population in conjunction with canonical HSC cell surface markers have confirmed the potential of CD150 ϩ selection for mouse HSC enrichment. 6 Although it has been suggested that some HSC activity may also be present in the CD150 Ϫ cell fraction of mouse cells, 6 data from multiple groups indicate that there is little or no long-term HSC activity in the CD150 Ϫ fraction of mouse hematopoietic cells. 1,3,4,[7][8][9][10] In humans, the CD34 cell surface marker is largely used in clinical applications for isolation of HSCs and progenitor cells, and as a predictor of graft HSC content in transplants. The combination CD34 ϩ CD38 Ϫ provides further enrichment (1 in 600 to 1 in 3500), 11 but the heterogeneity of this population precludes studies requiring levels of purity achieved in mouse models, such as comparing gene expression profiles or in situ histologic analyses. Given the use of SLAM receptors for identifying long-term repopulating HSCs in mice, 1,3,4,7-10 we hypothesized that this strategy may similarly facilitate the isolation of highly enriched populations of HSCs in humans and nonhuman p...

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CD38 expression by hematopoietic stem cells of newborn and juvenile mice

Cited by 24 publications

References 15 publications

Human cord blood long-term engrafting cells are CD34+ CD38−

Human cord blood long-term engrafting cells are CD34+ CD38−

ABC transporter activities of murine hematopoietic stem cells vary according to their developmental and activation status

Human and rhesus macaque hematopoietic stem cells cannot be purified based only on SLAM family markers

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