Recherches sur l’activité antibactérienne de certains agents anesthesiques

Barry, P P; Paiement, B.; Dubeau, Marie-France

doi:10.1007/bf03004109

Cited by 9 publications

(4 citation statements)

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“…At higher concentrations killing is complete. These observations are in conflict with those of Barry, Paiement and Dubeau (1964) who found that in the vapour state neither halothane nor methoxyflurane was tidal for Staph. aureus or E. coli.…”

Section: Discussioncontrasting

confidence: 95%

“…Liquid chloroform has been shown to be instantly bactericidal for enterobacteria (Bray, 1945;Hutner and Bjerknes, 1948), and chloroform vapour has been used for the sterilization of surfaces (Meynell and Meynell, 1965). On the other hand, Bany, Paiement and Dubeau (1964) have claimed that halothane and methoxyflurane vapour in clinical concentrations have no effect on Escherichia coli and Staphylococcus aureus. More recently Wardley-Smith and Nunn (1970) have shown that halothane causes a decrease in growth rate of bacteria growing in broth, while White and Dundas (1970) have demonstrated a reversible depression of light emission by halothane in Photobacterium phosphoreum.…”

mentioning

confidence: 99%

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The Antibacterial Action of Anaesthetic Vapours

Horton¹,

Sussman²,

Mushin³

1970

British Journal of Anaesthesia

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Section: Discussioncontrasting

confidence: 95%

mentioning

confidence: 99%

The Antibacterial Action of Anaesthetic Vapours

Horton¹,

Sussman²,

Mushin³

1970

British Journal of Anaesthesia

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“…saturation the anesthetics had almost no observable effect on viability of E. coli with the possible exception of halothane at 0.2x saturation for 300 min. Results of this study clearly indicate that halothane was the most effective against E. coli of the four chemicals tested and that none of the chemicals was effective unless used at a concentration above 0.2x saturation.Somewhat similar results were reported by Barry et al(1). They grew E. coli cells on blood agar and then treated the cultures with halothane.…”

supporting

confidence: 83%

Comparative Effects of Anesthetics on the Viability and Integrity of Escherichia coli ML30

Prior¹,

Fennema²,

Marth³

1975

Applied Microbiology

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Cells of Escherichia coli ML30 in a mineral salts medium were exposed to dichlorodifluoromethane (f-12), cyclopropane, halothane, or Ethrane at concentrations of 1.25, 0.2, 0.04, and 0.008x saturation for times up to 1,200 min, and at temperatures in the range of 2 to 37 C. When any of these anesthetics were applied for 300 min at 1.25x saturation, a substantial decrease in number of survivors occurred. Halothane was most bactericidal, cyclopropane and Ethrane were moderately bactericidal, and t-12 was least bactericidal. At saturation values of less than 1.0, none of the four anesthetics had an appreciable effect on viability of E. coli. Greatest increases in cell permeability occurred when anesthetics were used at saturation values of 1.25, and permeability changes generally decreased as the concentrations of the chemicals were reduced. In many instances, anesthetics in the vapor state caused significant increases in cell permeability but little or no loss of viability. This indicated that a close relationship did not exist between loss of viability and increased permeability. All four anesthetics caused E. coli to lose substantial and similar amounts of compounds absorbing at 260 nm. Release of compounds absorbing at 260 nm generally increased as the saturation value of a given chemical was increased. Halothane, Ethrane, and cyclopropane but not f-12 caused lysis of E. coli ML30. Considering all results, E. coli ML30 was damaged more by halothane or cyclopropane than by f-12 or Ethrane. When f-12 was applied at a saturation value of 1.25, the bactericidal effect on E. coli was much greater at 37 or 22 C than at 12 or 2 C.

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“…aureus (Table V) arrived at a similar conclusion and stated that growth of bacteria was virtually unaffected by concentrations of halothane in the range used during clinical anaesthesia. Barry et al 5 in their experiments exposed the organisms to the anaesthetic vapours on the surface of blood agar, while Wardley-Smith et at. 6 assessed bacterial growth rate either on nutrient agar plate or in nutrient broth.…”

Section: Individual Bacterial Resistance To Anaestheticsmentioning

confidence: 99%

The effect of volatile anaesthetics on bacterial growth

Mehta

Behr

Kenyon

1973

Canad. Anaesth. Soc. J.

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THERE HAS BEEN little investigation of the effect on bacterial growth of volatile anaesthetics in concentrations used in clinical anaesthesia. Waterhouse, 1 a Surgeon from Charing Cross Hospital, was the first person to mention the clinical results obtained by the use of di-ethyl ether in cases of various pyogenic infections. Topley 2 a Bacteriologist from the same hospital, showed that ether vapour exerts a definite bactericidal action on certain micro-organisms. John Bray 8 described a method of suppressing proteus and coliform bacteria on routine blood agar plates and came to the conclusion that ether vapour had a weak antiseptic ef [ect. Hunter et al. 4 used chloroform vapour as a preservative for culture media. Barry, Paiement and Dubeau 5 were the first to claim that halothane and methoxyflurane vapour in clinical concentrations had no antibacterial activity against Staphylococcus aureus and E. coll. More recently Wardley-Smith and Nunn 6 confirmed the findings of Barry et al. and showed that for certain species of non-pathogenic bacteria in broth, the effect on bacterial growth rate was negligible at clinical concentrations of halothane. On the other hand, Horton, Sussman and Mushin 7 exposed E. coli spread on cellulose acetate membranes to the vapours of volatile anaesthetic agents and showed that halothane, chloroform, trichloroethylene and methoxyflurane reduce the number of surviving organisms and that the reduction in survival was proportional to the concentration of the agent used.There are three main reasons why this knowledge of the effect of anaesthetics in clinical concentration on bacteria is useful. Firstly, it has clinical relevance to anaesthesia in patients with severe infection; secondly, it appeared likely that it might add to our scanty knowledge of the effect of anaesthetics at the molecular and cellular level and might reveal the possible mechanism of narcosis (WardleySmith et al. 19716 and White et al. 19708); and thirdly, it might throw light on the antibacterial action of anaesthetic vapours on organisms present on the surface of anaesthetic equipment, a factor which might be responsible for an incidence of post-operative pulmonary infection which is much lower than might be expected (Horton et al. ~ 1970). This paper describes studies of the effect of clinical concentrations of halothane, methoxyflurane and trichloroethylene on the growth of two species of bacteria grown on blood agar plates and cellulose acetate membranes.

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Recherches sur l’activité antibactérienne de certains agents anesthesiques

Cited by 9 publications

References 1 publication

The Antibacterial Action of Anaesthetic Vapours

The Antibacterial Action of Anaesthetic Vapours

Comparative Effects of Anesthetics on the Viability and Integrity of Escherichia coli ML30

The effect of volatile anaesthetics on bacterial growth

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