Receptors for C3 on B Lymphocytes: Possible Role in the Immune Response

117

Peripheral lymphocytes from patients with urinary bladder carcinoma and controls have been separated on the basis of rosette formation with sheep erythrocytes. The fractions were tested for tumor-specific cytotoxicity. The E rosette-forming cells of purity ⩾ 90% respond well in PHA-induced cytotoxicity but are totally inactive in the tumor assay. The non-E rosette-forming cells (purity ⩾ 91%) give enhanced activity in the tumor-specific cytotoxicity as well as in antibody-mediated target cell lysis in a model system. These data support the notion that the effector cells in cell-mediated immunity to carcinoma of the urinary bladder are members of the nonthymus-derived population of peripheral lymphocytes.

Section: Characterization Of Unfr Actionated and Fr Actionated Populamentioning

confidence: 90%

“…Complement Receptor.--The majority of nonthymus processed peripheral lymphoid cells possess a receptor for activated complement C3 (14,20,24,25). Unfracfionated cells were assayed for this receptor as described for human cells by Mellstedt et ai.…”

Section: Methodsmentioning

confidence: 99%

Lymphoid Cells Mediating Tumor-Specific Cytotoxicity to Carcinoma of the Urinary Bladder

O’Toole

Stejskal

Perlmann

et al. 1974

117

“…The discovery of an interaction between lymphocytes and the fixed C3 component of complement (2)(3)(4)(5), and the demonstration by Nussenzweig et al that C3 receptors are a feature of B-cell populations (6)(7)(8)(9)(10), raise the possibility that C3 might play a part in this process (11). Germinal centers of lymphoid tissue, in which the induction of antibody production m a y occur, contain B cells, fixed C3, and complexes capable of fixing C3 (12).…”

Section: (From the Department Of Pathology University Of Cambridge mentioning

confidence: 99%

Role of Complement in Induction of Antibody Production in Vivo

Pepys

1974

310

105

There has been considerable recent progress in understanding of the mechanisms involved in cooperation between T and B lymphocytes in the induction of humoral antibody formation (see for example 1), but much still remains to be learned. The discovery of an interaction between lymphocytes and the fixed C3 component of complement (2-5), and the demonstration by Nussenzweig et al. that C3 receptors are a feature of B-cell populations (6-10), raise the possibility that C3 might play a part in this process (11). Germinal centers of lymphoid tissue, in which the induction of antibody production m a y occur, contain B cells, fixed C3, and complexes capable of fixing C3 (12). I have previously shown that treatment of mice in vivo with the C3-cleaving protein of cobra venom (CoF) 1 suppresses thymus-dependent but not thymus-independent antibody production, suggesting a possible role for C3 in lymphocyte cooperation (11).

mentioning

confidence: 99%

“…The cells showed clumps floating in the media. The presence of surface-bound immunoglobulins (4-6) and of receptors for complement (7,8) and antigen-antibody complex on the membranes (8, 9) were considered to be markers for B lymphocytes.Recently, Minowada et al (10,11) succeeded in the establishment of the lymphoid cell lines (called MOLT and SOMMER) from the peripheral blood of patients with ALL. MOLT cells did not have immunoglobulins on the surface, nor receptors for antigen-antibody complex, but bound sheep red blood cells (SRBC) to form rosettes.…”

mentioning

confidence: 99%

Immunological Functions of Human T-Lymphoid Cell Line (Molt)

Takada

Minowada

1974

Recently much attention has been given to the classification of leukemia according to B-and T-cell origins. Wilson and Nossal (1) indicated that acute lymphoblastic leukemia (ALL) t might be a leukemia of T cells and chronic lymphatic leukemia (CLL) might be that of B cells. Since Iwakata and Grace (2) succeeded in the permanent culture of a hematopoietic cell line from the peripheral blood of a patient with acute myelogenous leukemia, many cell lines were successfully established. These cell lines produced immunoglobulins (3), and had receptors for complement and antigen-antibody complex. The cells showed clumps floating in the media. The presence of surface-bound immunoglobulins (4-6) and of receptors for complement (7,8) and antigen-antibody complex on the membranes (8, 9) were considered to be markers for B lymphocytes.Recently, Minowada et al. (10,11) succeeded in the establishment of the lymphoid cell lines (called MOLT and SOMMER) from the peripheral blood of patients with ALL. MOLT cells did not have immunoglobulins on the surface, nor receptors for antigen-antibody complex, but bound sheep red blood cells (SRBC) to form rosettes. None of the B-cell lines so far established bound SRBC. SOMMER T cells had similar characteristics to MOLT cells although the percentage of rosette formation was lower. The formation of spontaneous rosettes with SRBC in humans was considered to be a marker for T cells (6, 12). MOLT and SOMMER cells do not form clumps in the media. Furthermore, it has been shown that cytotoxicity of rabbit anti-MOLT serum was absorbed with fresh human thymocytes, but not with human B cells or liver (unpublished observation). We have recently shown that MOLT cells have antigenic determinants shared with human brain (13). Shared antigenic determinants between thymocyte and brain have been reported in the rat and mouse (14, 15). As Minowada and Moore have shown (11), rabbit anti-MOLT sera specifically killed cells from most of the patients with ALL, but did not kill cells from most of the patients with CLL.