Lymphoid Cells Mediating Tumor-Specific Cytotoxicity to Carcinoma of the Urinary Bladder

Abstract

Peripheral lymphocytes from patients with urinary bladder carcinoma and controls have been separated on the basis of rosette formation with sheep erythrocytes. The fractions were tested for tumor-specific cytotoxicity. The E rosette-forming cells of purity ⩾ 90% respond well in PHA-induced cytotoxicity but are totally inactive in the tumor assay. The non-E rosette-forming cells (purity ⩾ 91%) give enhanced activity in the tumor-specific cytotoxicity as well as in antibody-mediated target cell lysis in a model system. These data support the notion that the effector cells in cell-mediated immunity to carcinoma of the urinary bladder are members of the nonthymus-derived population of peripheral lymphocytes.

A cell line from an anaplastic transitional cell carcinoma of human urinary bladder

“…It has been our experience that cells from bladder tumours which metastasize in vivo are easily established in long-term tissue culture. Conversely, cells from superficial localized TCC were much slower to grow in vitro, and they rarely survived It has previously been observed that lymphoid cells from patients with lowstage TCC selectively destroy cells from TCC (O'Toole et al, 1974). In those experiments, TCC target cells were from the lines T24, RT4 and J82.…”

“…The results have suggested that cells derived from TCC retain tumour-associated antigens (Bubenik et al, 1973;O'Toole et al, 1974;Hakala and Lange, 1974). The majority of these studies have employed the established TOC cell lines, T24 and/or RT4.…”

Antibody-dependent cell-mediated cytotoxicity in selected autoimmune diseases.

“…This third population, generally called K or killer cells, may cause -tissue destruction by the mechanism of antibody-dependent cellmediated cytotoxicity (ADCC).1 ADCC has been implicated as an important immunologic defense mechanism against malignancy (3). Its involvement in renal transplant rejection (4) and in autoimmune tissue destruction (5,6) has been postulated.…”

Human antibody-dependent cellular cytotoxicity. Isolation and identification of a subpopulation of peripheral blood lymphocytes which kill antibody-coated autologous target cells.

“…In addition, recent studies have also shown that patients with transitional cell carcinoma (TCC) can develop antibody which specifically renders cultured TCC cell lines susceptible to ADCC killing by patient lymphocytes (27,28). Thus, evidence is mounting that ADCC is an ubiquitous immune cytotoxic mechanism, at least in vitro.…”