A cell line from an anaplastic transitional cell carcinoma of human urinary bladder

Nayak, Shankar K.; O’Toole, Carol; Price, Zane H.

doi:10.1038/bjc.1977.21

Cited by 77 publications

(43 citation statements)

References 12 publications

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“…3). In this respect, J82 cells resembled the line TCC SUP also derived from poorly differentiated TCC (Nayak et al, 1977), but differed from cultured squamous carcinoma of the bladder (O'Toole et al, 1976).…”

Section: Micromorphologymentioning

confidence: 92%

“…J82 cells have been tested routinely during the last 5 years as targets for allogeneic lymphoid-cell cytotoxicity. Effector cells from some patients with localized TCC were cytotoxic toward primary cultured J82 cells Nayak et al, 1977). However, after long-term in vitro culture this type of reaction was no longer detected (O'Toole, 1977).…”

mentioning

confidence: 96%

“…The majority of tests reported to date have described allogeneic lymphocyte-mediated cytotoxicity to TCC cells. The results indicate that not all cell lines derived from bladder carcinomas express the antigens detected by lymphoid-cell cytotoxicity (Nayak et al, 1977;O'Toole, 1977). Further, serum antibodies have been identified in patients with squamous carcinoma of the bladder which do not react with TCC (Sofen and O'Toole, 1978).…”

mentioning

confidence: 99%

“…In general, cell lines from bladder carcinomas established in vitro retain the morphological attributes of the original tumour (Rigby and Franks, 1970;Bubenik et al, 1973;O'Toole et al, 1976;Nayak et al, 1977). TCC cell lines have been used extensively in the search for host immunity to tumour.…”

mentioning

confidence: 99%

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Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma

O’Toole¹,

Price²,

Ohnuki³

et al. 1978

Br J Cancer

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138

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Summary.-A cell line (J82) was derived from a poorly differentiated, invasive, transitional-cell carcinoma, Stage T3. The cells have been propagated in vitro for 5 years and showed 100% aneuploidy and a mixed epithelial-fibroblastic morphology.The majority of cells contained 2Y chromosomes and several distinctive markers. Peripheral-blood lymphocytes from the donor of the J82 cells were tested sequentially for cytotoxicity toward autologous and allogeneic tumour cells. Autologous cyto Foxicity was detected against J82 cells in early in vitro passage. Allogeneic lymphocytes from some patients with transitional-cell carcinoma were also cytotoxic to J82 cells in primary culture. However, selective cytotoxicity by lymphoid cells from bladdercarcinoma patients was not detected against J82 cells in long-term tissue culture.

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Section: Micromorphologymentioning

confidence: 92%

mentioning

confidence: 96%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma

O’Toole¹,

Price²,

Ohnuki³

et al. 1978

Br J Cancer

Self Cite

138

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“…For this purpose, we chose an undifferentiated bladder cancer cell line TCCSUP (grade 4), which was taken from a patient who subsequently developed metastases. 22 Bladder cancer cell line TCCSUP and primary cells from normal urothelium were exposed mRNA levels from individual samples in groups pTa (n ϭ 8) and pT1 (n ϭ 6) were averaged and compared to the mRNA levels of samples in groups pT2 (n ϭ 2), pT3 (n ϭ 7) and pT4 (n ϭ 5). The highest mRNA levels of CXCR4 occurred in invasive and locally advanced bladder cancer (pT2-T4), while superficial bladder cancer (pTa and pT1) displayed low CXCR4 mRNA levels.…”

Section: Cxcr4 Chemokine Receptor and Its Ligand Cxcl12 Mediate Enhanmentioning

confidence: 99%

CXCR4 expression reflects tumor progression and regulates motility of bladder cancer cells

Retz

Sidhu

Blaveri

et al. 2004

Intl Journal of Cancer

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Transitional cell carcinoma of the urinary bladder remains life threatening due to the high occurrence of metastases. Emerging evidence suggests that chemokines and their receptors play a critical role in tumor metastases. In our study, we performed a systematic analysis of the mRNA and protein expression levels of all 18 chemokine receptors in normal urothelium and bladder cancer. CXCR4 was the only chemokine receptor whose mRNA expression level was upregulated in bladder cancer cell lines as well as in invasive and locally advanced bladder cancer tissue samples (pT2-pT4). In contrast, superficial bladder tumors (pTa and pT1) displayed low CXCR4 expression levels and normal urothelial cells were negative for CXCR4. Immunohistochemistry of a bladder cancer tissue microarray (TMA) confirmed that a subgroup of invasive bladder cancers revealed a high CXCR4 protein expression, while superficial bladder tumors showed low immunoreactivity. To investigate the functional significance of CXCR4 expression, we performed migration and invasion assays. Exposure of CXCR4-positive bladder cancer cells to CXCL12 in a Boyden chamber type assay provoked a significant increase in migration as well as invasion across a Matrigel barrier. Enhanced migration and invasion were inhibited by a CXCR4-specific blocking antibody. In contrast, normal urothelial cells did not respond to CXCL12 and lacked chemotactic migration. In conclusion, bladder cancer cells express CXCR4 progressively with advanced tumorigenesis and this receptor interacts with CXCL12 to mediate tumor chemotaxis and invasion through connective tissue. These properties identify CXCR4 as a potential target for the attenuation of bladder cancer metastases. © 2004 Wiley-Liss, Inc.Key words: chemokine; bladder cancer; metastasis; migration; invasion Transitional cell carcinoma of the bladder is the most frequent tumor of the urinary tract. Despite advances in early detection and therapy, bladder cancer remains life threatening due to the high occurrence of metastases. Radical cystectomy is the preferred treatment for muscle-invasive bladder cancer in the United States, Japan and some countries of Europe. However, at least 50% of patients with locally advanced disease are expected to develop systemic progression within 3 years. 1 Patients suffering from metastatic bladder cancer are commonly treated with systemic chemotherapy. Analyzing survival rates from standard chemotherapy schedules such as M-VAC (methotrexate, vinblastine, adriamycin and cisplatin), results are more or less discouraging. In the Loehrer trial, only 5 of 133 (3.7%) of the patients randomized to M-VAC were alive at the 6-year follow-up time. These results indicate that patients with systemic metastases continue to be incurable by chemotherapy. 2,3 Despite the fact that it is the metastases rather than the primary tumors that cause most cancer deaths, there has been little progress in identifying drugs to specifically block metastases.Metastasis is not a simple phenomenon, but a highly organized process com...

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The Establishment and Characterization of Bladder Cancer Cultures In Vitro

Fu¹,

Schwarze²,

Reznikoff³

et al. 2003

Culture of Human Tumor Cells

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A cell line from an anaplastic transitional cell carcinoma of human urinary bladder

Cited by 77 publications

References 12 publications

Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma

Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma

CXCR4 expression reflects tumor progression and regulates motility of bladder cancer cells

The Establishment and Characterization of Bladder Cancer Cultures In Vitro

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