Receptor-targeted co-transport of DNA and magnetic resonance contrast agents

Kayyem, Jon F.; Kumar, R.; Fraser, Scott E.; Meade, Thomas J.

doi:10.1016/1074-5521(95)90126-4

Cited by 76 publications

(35 citation statements)

References 18 publications

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“…Multifunctional NPs comprising of paramagnetic CA as well as a luminescent fluorophore blend the high sensitivity of optical imaging with 3-dimensional MR imaging of the body tissues. Also, the low sensitivity of MRI and little information of anatomical background in optical imaging are compensated by each other [13][14]. Such multimodal NPs usually comprise of an active core and a biocompatible surface…”

Section: Introductionmentioning

confidence: 98%

FITC-Dextran entrapped and silica coated gadolinium oxide nanoparticles for synchronous optical and magnetic resonance imaging applications

Kumar

Meena

Hazari

et al. 2016

International Journal of Pharmaceutics

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Section: Introductionmentioning

confidence: 98%

FITC-Dextran entrapped and silica coated gadolinium oxide nanoparticles for synchronous optical and magnetic resonance imaging applications

Kumar

Meena

Hazari

et al. 2016

International Journal of Pharmaceutics

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“…However, the application of these compounds has been limited, and they have been utilized mostly to monitor cell movement in embryogenesis and embryonic cell lineage analysis (19). Covalent attachment of Gd-DOTA to the HIV-Tat peptide was used for lymphocyte labeling by receptor-mediated endocytosis (20), and the attachment of Gd-DTPA to poly-L-lysinetransferrin-DNA enabled labeling of the erythroleukemia cell line via cotransport of the contrast material with DNA during transfection (21). At present, most work in this field has focused on the detection of stem cells or cells belonging to the hematopoietic system.…”

mentioning

confidence: 99%

Labeling fibroblasts with biotin‐BSA‐GdDTPA‐FAM for tracking of tumor‐associated stroma by fluorescence and MR imaging

Granot¹,

Kunz-Schughart

Neeman

2005

Magnetic Resonance in Med

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Fibroblasts at the tumor-host interface can differentiate into myofibroblasts and pericytes, and contribute to the guidance and stabilization of endothelial sprouts. After intravenous administration of biotin-BSA-GdDTPA-FAM in mice with subcutaneous MLS human ovarian carcinoma tumors, the distribution of the macromolecular MRI/optical contrast material was confined to blood vessels in normal tissues, while it co-registered with ␣SMA-positive stroma tracks within the tumor. These ␣SMA-positive tumor-associated myofibroblasts and pericytes showed uptake of the contrast material into intracellular granules. We evaluated the use of this contrast material for in vitro labeling of tumor fibroblasts as an approach for tracking their involvement in angiogenesis. Fluorescence microscopy demonstrated internalization of the contrast material, and MRI revealed a significant increase in the R 1 relaxation rate of labeled fibroblasts. R 1 not only remained elevated for 2 weeks in culture, it also increased with cell proliferation, indicating prolonged retention of the contrast material and subsequent intracellular processing and redistribution of the material, and thereby enhancing MR contrast. Moreover, cells that were labeled ex vivo with MR contrast material and co-inoculated with tumor cells in mice were detected in vivo by MRI. Uptake of the contrast material was suppressed by nystatin, suggesting internalization by caveolae-mediated endocytosis. This study shows that labeling of fibroblasts with biotin-BSA-GdDTPA-FAM is feasible and would allow noninvasive in vivo tracking of fibroblasts during tumor angiogenesis and vessel maturation. Magn Reson Med 54:789 -797, 2005.

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“…Microinjected contrast agents have allowed microscopic magnetic resonance imaging (MRI) to visualize cell lineages and track neural development in vivo in developing embryos (6). Other examples of targeted͞functional agents include lipophilic agents, which target the liver (4,5,7,8); macromolecular complexes, which function as blood pool markers (9); lymphotropic agents (10); magnetoimmuno agents, which target specific cell surface receptors (11,12); and, recently, manganese ions, which enter through calcium channels of neurons, have been used to highlight regions of brain activation (13).…”

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confidence: 99%

A model for MRI contrast enhancement using T ₁ agents

Ahrens

Rothbächer

Jacobs

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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Contrast in MRI relies on differences in the local environment of water and is often enhanced by using contrast agents. We present a simple model for evaluating the minimal contrast agent concentration required to produce ''satisfactory'' contrast enhancement in magnetic resonance images. Previous strategies have been based largely on empirical results for specific systems. The present tissue contrast model (TCM) can be applied to ''conventional,'' targeted, or biochemically responsive agents. The model results are formulated so that only a small number of parameters are required to analyze a given scenario. The TCM is a particularly useful tool in the development of new classes of magnetic resonance contrast media. These agents will have the ability to target specific cells or tissue, and perhaps be able to report on their physiological status. As an example of the applicability of the TCM, we test it against in vivo magnetic resonance microscopy results in frog embryos that have focal cell populations labeled with contrast agent by using calibrated single-cell microinjection techniques.

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Receptor-targeted co-transport of DNA and magnetic resonance contrast agents

Abstract: The development of this class of particles permits the use of novel protocols by which genes for genetic therapy and agents for MRI contrast are co-transported. These protocols may allow non-invasive MRI monitoring of DNA delivery for gene therapy in real time.

Cited by 76 publications

References 18 publications

FITC-Dextran entrapped and silica coated gadolinium oxide nanoparticles for synchronous optical and magnetic resonance imaging applications

FITC-Dextran entrapped and silica coated gadolinium oxide nanoparticles for synchronous optical and magnetic resonance imaging applications

Labeling fibroblasts with biotin‐BSA‐GdDTPA‐FAM for tracking of tumor‐associated stroma by fluorescence and MR imaging

A model for MRI contrast enhancement using T ₁ agents

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Receptor-targeted co-transport of DNA and magnetic resonance contrast agents

Abstract: The development of this class of particles permits the use of novel protocols by which genes for genetic therapy and agents for MRI contrast are co-transported. These protocols may allow non-invasive MRI monitoring of DNA delivery for gene therapy in real time.

Cited by 76 publications

References 18 publications

FITC-Dextran entrapped and silica coated gadolinium oxide nanoparticles for synchronous optical and magnetic resonance imaging applications

FITC-Dextran entrapped and silica coated gadolinium oxide nanoparticles for synchronous optical and magnetic resonance imaging applications

Labeling fibroblasts with biotin‐BSA‐GdDTPA‐FAM for tracking of tumor‐associated stroma by fluorescence and MR imaging

A model for MRI contrast enhancement using T 1 agents

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A model for MRI contrast enhancement using T ₁ agents