Receptor for detection of a Type II sex pheromone in the winter moth Operophtera brumata

Zhang, Dan Dan; Wang, Hong Lei; Schultze, Anna; Froß, Heidrun; Francke, Wittko; Krieger, Jürgen; Löfstedt, Christer

doi:10.1038/srep18576

Cited by 36 publications

(49 citation statements)

References 61 publications

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“…cunea . Differentiation of sex pheromones in various moth species has occurred over many millennia, using diverse biosynthetic pathways involving different enzymes, substrates, and binding sites, resulting in the two major recognized pheromone types [ 29 ]. To date, little is known about the olfactory proteins repertoire species using Type-II pheromones.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the Antennal Transcriptome and Insights into Olfactory Genes in Hyphantria cunea (Drury)

et al. 2016

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Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) is an invasive insect pest which, in China, causes unprecedented damage and economic losses due to its extreme fecundity and wide host range, including forest and shade trees, and even crops. Compared to the better known lepidopteran species which use Type-I pheromones, little is known at the molecular level about the olfactory mechanisms of host location and mate choice in H. cunea, a species using Type-II lepidopteran pheromones. In the present study, the H. cunea antennal transcriptome was constructed by Illumina Hiseq 2500TM sequencing, with the aim of discovering olfaction-related genes. We obtained 64,020,776 clean reads, and 59,243 unigenes from the analysis of the transcriptome, and the putative gene functions were annotated using gene ontology (GO) annotation. We further identified 124 putative chemosensory unigenes based on homology searches and phylogenetic analysis, including 30 odorant binding proteins (OBPs), 17 chemosensory proteins (CSPs), 52 odorant receptors (ORs), 14 ionotropic receptors (IRs), nine gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs). We also found many conserved motif patterns of OBPs and CSPs using a MEME system. Moreover, we systematically analyzed expression patterns of OBPs and CSPs based on reverse transcription PCR and quantitative real time PCR (RT-qPCR) with RNA extracted from different tissues and life stages of both sexes in H. cunea. The antennae-biased expression may provide a deeper further understanding of olfactory processing in H. cunea. The first ever identification of olfactory genes in H. cunea may provide new leads for control of this major pest.

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Section: Introductionmentioning

confidence: 99%

“…To date, little is known about the olfactory proteins repertoire species using Type-II pheromones. Did they evolve a novel receptors to perceive the Type-II sex pheromones, or were existing pheromone receptors were recruited for detection of the new ligands [ 29 ]? We assume that specific H .…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Antennal Transcriptome and Insights into Olfactory Genes in Hyphantria cunea (Drury)

et al. 2016

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“…4). One was the so-called PR clade that, as previously observed, contained all type I PRs characterized so far (except SlitOR5) as well as two type II PRs (Zhang et al, 2016). The other three lineages harboring PRs consisted of one containing SlitOR5, one containing EgriOR31 (a type II PR from the geometrid Ectropis grisescens; Li et al, 2017) and one containing EsemOR3 and 5 (type 0 PRs from the non-dytrisian moth Eriocrania semipurpurella; Yuvaraj et al, 2017).…”

Section: A Novel Lineage Of Candidate Moth Pheromone Receptorsmentioning

confidence: 56%

A novel lineage of candidate pheromone receptors for sex communication in moths

Bastin-Héline

Fouchier

Cao

et al. 2019

Preprint

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Sex pheromone receptors (PRs) are key players in chemical communication between mating partners in insects. In the highly diversified insect order Lepidoptera, male PRs tuned to female-emitted type I pheromones (which make up the vast majority of pheromones identified) form a dedicated subfamily of odorant receptors (ORs). Here, using a combination of heterologous expression and in vivo genome editing methods, we bring functional evidence that at least one moth PR does not belong to this subfamily but to a distantly related OR lineage. This PR, identified in the cotton leafworm Spodoptera littoralis, is over-expressed in male antennae and is specifically tuned to the major sex pheromone component emitted by females. Together with a comprehensive phylogenetic analysis of moth ORs, our functional data suggest two independent apparitions of PRs tuned to type I pheromones in Lepidoptera, opening up a new path for studying the evolution of moth pheromone communication.

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“…Artificial release of lacewings is a well‐known method for suppressing aphids (Zhang et al ., ), but it is difficult to establish populations in the field. Understanding the molecular recognition mechanisms of CpalOBPs and the behavioural responses of C. pallens to plant volatiles and the aphid alarm pheromone has important implications for population establishment after release.…”

Section: Discussionmentioning

confidence: 99%

“…It is found in many different agro‐ecosystems including tea gardens, maize, cotton and rice (Brooks, ; Bai et al ., ). Artificial lacewing release is a well‐known method for suppressing aphids (Zhang et al ., ), but it is difficult to establish lacewing populations in the field. Previous studies have reported that plant‐derived compounds, pest‐induced volatiles and aphid alarm pheromones are used by predators and parasitoids as foraging cues (Al Abassi et al ., ; Xu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Three odorant binding proteins may regulate the behavioural response of Chrysopa pallens to plant volatiles and the aphid alarm pheromone (E)‐β‐farnesene

Zhang

Cai

et al. 2017

Insect Molecular Biology

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Artificial Chrysopa pallens release is a well‐known method for suppressing aphids, but it is difficult to establish lacewing populations in the field. Understanding the functions of C. pallens odorant‐binding proteins (CpalOBPs) and behavioural responses of C. pallens to plant volatiles and aphid alarm pheromone (E)‐ß‐farnesene has important implications for population establishment after lacewing release. Based on our previous study, five antennae‐enriched CpalOBPs were selected. Sequence alignment and phylogenetic analysis revealed that these five CpalOBPs were Classic OBPs and separated into different clades. Of them, CpalOBP10 clustered in the same clade with aphid OBP7, which mediates the perception of green leaf volatiles and (E)‐ß‐farnesene. Ligand‐binding assays showed 31 compounds, including plant‐derived compounds, pest‐induced volatiles and (E)‐ß‐farnesene, had high binding affinities for at least one of these five CpalOBPs. Of the 31 compounds, the pest‐induced volatiles (Z)−3‐hexenyl hexanoate and 2‐hexyl‐1‐decanol, used in host location by the black bean aphid, elicited significant attractive behavioural responses from C. pallens. Conversely, (E)‐ß‐farnesene elicited strongly repellent behavioural responses. It is conceivable that C. pallens utilizes plant‐derived compounds, pest‐induced volatiles and (E)‐ß‐farnesene as foraging cues. Our studies provide new insights into the interrelationships amongst C. pallens, its prey and the host plants. Compounds that elicited significant behavioural responses from C. pallens were also identified.

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Receptor for detection of a Type II sex pheromone in the winter moth Operophtera brumata

Cited by 36 publications

References 61 publications

Analysis of the Antennal Transcriptome and Insights into Olfactory Genes in Hyphantria cunea (Drury)

Analysis of the Antennal Transcriptome and Insights into Olfactory Genes in Hyphantria cunea (Drury)

A novel lineage of candidate pheromone receptors for sex communication in moths

Three odorant binding proteins may regulate the behavioural response of Chrysopa pallens to plant volatiles and the aphid alarm pheromone (E)‐β‐farnesene

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