Three odorant binding proteins may regulate the behavioural response of Chrysopa pallens to plant volatiles and the aphid alarm pheromone (E)‐β‐farnesene

Abstract

Artificial Chrysopa pallens release is a well‐known method for suppressing aphids, but it is difficult to establish lacewing populations in the field. Understanding the functions of C. pallens odorant‐binding proteins (CpalOBPs) and behavioural responses of C. pallens to plant volatiles and aphid alarm pheromone (E)‐ß‐farnesene has important implications for population establishment after lacewing release. Based on our previous study, five antennae‐enriched CpalOBPs were selected. Sequence alignment and phylogenetic analysis revealed that these five CpalOBPs were Classic OBPs and separated into different clades. Of them, CpalOBP10 clustered in the same clade with aphid OBP7, which mediates the perception of green leaf volatiles and (E)‐ß‐farnesene. Ligand‐binding assays showed 31 compounds, including plant‐derived compounds, pest‐induced volatiles and (E)‐ß‐farnesene, had high binding affinities for at least one of these five CpalOBPs. Of the 31 compounds, the pest‐induced volatiles (Z)−3‐hexenyl hexanoate and 2‐hexyl‐1‐decanol, used in host location by the black bean aphid, elicited significant attractive behavioural responses from C. pallens. Conversely, (E)‐ß‐farnesene elicited strongly repellent behavioural responses. It is conceivable that C. pallens utilizes plant‐derived compounds, pest‐induced volatiles and (E)‐ß‐farnesene as foraging cues. Our studies provide new insights into the interrelationships amongst C. pallens, its prey and the host plants. Compounds that elicited significant behavioural responses from C. pallens were also identified.

Two Odorant-Binding Proteins of the Dark Black Chafer (Holotrichia parallela) Display Preferential Binding to Biologically Active Host Plant Volatiles

“…However, all the volatiles tested in this study showed a relatively weak binding affinity (Ki > 20 μM) to HparOBP49. Compensation effects may exist between HparOBP20 and HparOBP49, as observed for Cnaphalocrocis medinalis OBP2 and OBP3 ( Sun et al, 2016 ), and Chrysopa pallens OBP3, -6 and -10 ( Li et al, 2017 ).…”

Non‐destructive characterization of egg odor and fertilization status by SPME/GC‐MS coupled with electronic nose

“…Cedrene had a specific relation with antimicrobial, Which has always been considered as the derived compounds of essential oils . The intensity of cedrene from UF eggs was much higher than that from Fd eggs, which could be interpreted as the faster emission rate in UF eggs and the odorant‐binding proteins in eggshell could selectively absorb and release odors …”

“…32 The intensity of cedrene from UF eggs was much higher than that from Fd eggs, which could be interpreted as the faster emission rate in UF eggs and the odorant-binding proteins in eggshell could selectively absorb and release odors. 33 In Fd and UF eggs, significant changes in seven protein spots were identified at an early stage of incubation, and the intensity of 24th spot (ovoinhibitor) was significantly changed on day 0 of incubation. 18 In Fd and UF eggs, more than 40 protein spots significantly changed in eggshell membrane (ESM) at day 0 of incubation.…”

Combining E-β-farnesene and methyl salicylate release with wheat-pea intercropping enhances biological control of aphids in North China

“…Chrysopa pallens (Li et al 2017). However, previous electroantennogram results showed that the antennae of Chrysoperla carnea are highly sensitive to EBF (Zhu et al 1999), which can support the increased abundance of lacewings observed in EBF treatment compared to Control.…”