Receptor-based screening assays for the detection of antibiotics residues – A review

Ahmed, Saeed; Ning, Jianan; Cheng, Guyue; Ahmad, Ijaz; Jun, Li; Liu, Mingyue; Qu, Wei; Iqbal, Mujahid; Shabbir, Muhammad Abu Bakr; Zhang, Yuan

doi:10.1016/j.talanta.2017.01.057

Cited by 65 publications

(41 citation statements)

References 95 publications

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“…In recent years, great attempts have been made toward the investment and development of high-throughput assays for the screening of antibiotics, including receptor-based screening for antibiotics residue (i.e., radioreceptor assay, enzyme labeling assays, colloidal gold receptor assay, enzyme colorimetry assay, and biosensor assay). These assays make the detection of antibiotics faster, easier, and more accurate [14]. The use of biosensors is one of the most widely spread techniques applied for the detection of antibiotics and contaminants in foods and environment [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

On Column Binding a Real-Time Biosensor for β-lactam Antibiotics Quantification

Abdullah

Rachid²

2020

Molecules

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This work aimed to develop accurate, quick, and practical tools for the detection of residues of penicillin G antibiotic in biological and non-biological samples. The assays were developed based on the binding mechanism of β-lactam to penicillin-binding proteins; samples of different concentrations of penicillin G were incubated with in vitro expressed 6X-Histidine-tagged soluble penicillin-binding protein (PBP2x*) of Streptococcus pneumoniae (S. pneumoniae), whereby penicillin G in samples specifically binds to PBP2x*. The fluorescent-labeled β-lactam analogue Bocillin FL was used as a competent substrate, and two different routes estimated the amounts of the penicillin G. The first route was established based on the differences in the concentration of non-bounded Bocillin FL molecules within the reactions while using a real-time polymerase chain reaction (PCR)-based method for fluorescence detection. The second route depended on the amount of the relative intensity of Bocillin FL bounded to Soluble PBP-2x*, being run on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-page), visualized by a ChemiDoc-It®2 Imager, and quantified based on the fluorescence affinity of the competent substrate. While both of the methods gave a broad range of linearity and high sensitivity, the on column based real-time method is fast, non-time consuming, and highly sensitive. The method identified traces of antibiotic in the range 0.01–0.2 nM in addition to higher accuracy in comparison to the SDS-based detection method, while the sensitivity of the SDS-based method ranged between 0.015 and 2 µM). Thus, the on column based real time assay is a fast novel method, which was developed for the first time based on the binding inhibition of a fluorescence competitor material and it can be adapted to screen traces of penicillin G in any biological and environmental samples.

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Section: Introductionmentioning

confidence: 99%

On Column Binding a Real-Time Biosensor for β-lactam Antibiotics Quantification

Abdullah

Rachid²

2020

Molecules

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“…Specifically, TtgR is able to bind with antibiotics, flavonoids and aromatic solvents. [108][109][110] For instance, the antimicrobial properties of the flavonoids of quercetin, naringenin and phloretin lead to their abilities of binding to TtgR. 111 The binding between the effector and TtgR often leads to the dissociation and the transcriptional activation of TtgABC and TtgR.…”

Section: Introductionmentioning

confidence: 99%

Theoretical Understanding of the Thermodynamics and Interactions in Transcriptional Regulator TtgR-Ligand Binding

Sun

Wang²,

Zhang³

2019

Preprint

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<p> The transcriptional regulator TtgR belongs to the TetR family of transcriptional repressors. It depresses the transcription of the TtgABC operon and itself and thus regulates the extrusion of noxious chemicals with efflux pumps in bacterial cells. As the ligand binding domain of TtgR is rather flexible, it can bind with a number of structurally diverse ligands, such as antibiotics, flavonoids and aromatic solvents. In the current work, we perform equilibrium and nonequilibrium alchemical free energy simulation to predict the binding affinities of a series of ligands targeting the TtgR protein and the agreement between the theoretical prediction and the experimental result is observed. End-point methods of MM/PBSA and MM/GBSA are also employed for comparison. We further study the interaction maps and identify important interactions in the protein-ligand binding cases. The current work sheds light on atomic and thermodynamic understanding on the TtgR-ligand interactions.</p>

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“…CAP residues in animals can be transmitted to humans through food and could predispose humans to various diseases. Due to its severe toxicity and side effects for the human immune and hematopoietic systems [5], CAP has been banned from animal breeding and aquatic products [6]. Currently, methods for detection of CAP residues include microbiological methods, chromatography [7][8][9][10][11], liquid-chromatography tandem mass spectrometry (LC-MS) [12,13], and chromatography combined with immunoassay [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum

Zhao

Chanling

Sun

et al. 2019

Journal of Analytical Methods in Chemistry

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Chloramphenicol-(CAP-) restricted access media-molecularly imprinted polymers (CAP-RAM-MIPs) were prepared by precipitation polymerization using CAP as a template molecule, 2-diethylaminoethyl methacrylate (DEAEM) as a functional monomer, ethylene glycol dimethyl acrylate (EDMA) as a crosslinking agent, glycidyl methacrylate (GMA) as an outer hydrophilic functional monomer, and acetonitrile as a pore former and solvent. e CAP-RAM-MIPs were successfully characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis. e adsorption performance was investigated in detail using static, dynamic, and selective adsorption experiments. Adsorption equilibrium could be reached within 11 min. e CAP-RAM-MIPs had a high adsorption rate and good specific adsorption properties. Scatchard fitting curves indicated there were two binding sites for CAP-RAM-MIPs. Adsorption was Freundlich multilayer adsorption and consistent with the quasi-second kinetic model. Using CAP-RAM-MIPs for selective separation and enrichment CAP in bovine serum in combination with highperformance liquid chromatography (HPLC), CAP recovery ranged from 94.1 to 97.9% with relative standard deviations of 0.7-1.5%.is material has broad application prospects in enrichment and separation.

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Receptor-based screening assays for the detection of antibiotics residues – A review

Cited by 65 publications

References 95 publications

On Column Binding a Real-Time Biosensor for β-lactam Antibiotics Quantification

On Column Binding a Real-Time Biosensor for β-lactam Antibiotics Quantification

Theoretical Understanding of the Thermodynamics and Interactions in Transcriptional Regulator TtgR-Ligand Binding

Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum

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