Abstract:Chloramphenicol-(CAP-) restricted access media-molecularly imprinted polymers (CAP-RAM-MIPs) were prepared by precipitation polymerization using CAP as a template molecule, 2-diethylaminoethyl methacrylate (DEAEM) as a functional monomer, ethylene glycol dimethyl acrylate (EDMA) as a crosslinking agent, glycidyl methacrylate (GMA) as an outer hydrophilic functional monomer, and acetonitrile as a pore former and solvent. e CAP-RAM-MIPs were successfully characterized by Fourier-transform infrared spectroscopy, … Show more
“…The specific surface area of the poly(GMA‐ co ‐EDMA) microspheres was measured to be 23.24 m 2 /g, while the specific surface area of the MIP was found to be 8.55 m 2 /g. The difference in surface area and pore size and volume between the poly(GMA‐ co ‐EDMA) microspheres and MIP is likely related to the imprinting effect [35].…”
The addition of bisphenol A has been frequently used in industrial manufacturing because it imparts plastic products with characteristics such as transparency, durability, and excellent impact resistance. However, its widespread use raises concerns about potential leakage into the surrounding environment, which poses a significant risk to human health. In this study, molecularly imprinted polymers with specific recognition of bisphenol A were synthesized through surface-initiated atom transfer radical polymerization using poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) as the substrate, bisphenol A as the template molecule, 4-vinylpyridine as the monomer, and ethylene glycol dimethacrylate as the cross-linker. The bisphenol A adsorption capacity was experimentally investigated, and the kinetic analysis of the molecularly imprinted polymers produced an adsorption equilibrium time of 25 min, which is consistent with the pseudo-second-order kinetic model. The results of the static adsorption experiments exhibited consistency with the Langmuir adsorption model, revealing a maximum adsorption capacity of 387.2 μmol/g. The analysis of molecularly imprinted polymers-enriched actual samples using high-performance liquid chromatography demonstrated excellent selectivity for bisphenol A, with a linear range showing 93.4%-99.7% recovery and 1.1%-6.4% relative standard deviation, demonstrating its high potential for practical bisphenol A detection and enrichment applications.
“…The specific surface area of the poly(GMA‐ co ‐EDMA) microspheres was measured to be 23.24 m 2 /g, while the specific surface area of the MIP was found to be 8.55 m 2 /g. The difference in surface area and pore size and volume between the poly(GMA‐ co ‐EDMA) microspheres and MIP is likely related to the imprinting effect [35].…”
The addition of bisphenol A has been frequently used in industrial manufacturing because it imparts plastic products with characteristics such as transparency, durability, and excellent impact resistance. However, its widespread use raises concerns about potential leakage into the surrounding environment, which poses a significant risk to human health. In this study, molecularly imprinted polymers with specific recognition of bisphenol A were synthesized through surface-initiated atom transfer radical polymerization using poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) as the substrate, bisphenol A as the template molecule, 4-vinylpyridine as the monomer, and ethylene glycol dimethacrylate as the cross-linker. The bisphenol A adsorption capacity was experimentally investigated, and the kinetic analysis of the molecularly imprinted polymers produced an adsorption equilibrium time of 25 min, which is consistent with the pseudo-second-order kinetic model. The results of the static adsorption experiments exhibited consistency with the Langmuir adsorption model, revealing a maximum adsorption capacity of 387.2 μmol/g. The analysis of molecularly imprinted polymers-enriched actual samples using high-performance liquid chromatography demonstrated excellent selectivity for bisphenol A, with a linear range showing 93.4%-99.7% recovery and 1.1%-6.4% relative standard deviation, demonstrating its high potential for practical bisphenol A detection and enrichment applications.
“…6 a that MIPs reached an equilibrium within 60 min. This is because the recognition sites produced by the surface imprinting are distributed on the polymer surface and therefore they are highly accessible and mass transfer resistance is low, which allow to achieve an adsorption equilibrium 26 . Figure 6 ( a ) Adsorption kinetics of MIP/NIP and ( b ) adsorption curves for analytes at different concentrations (10–30 µg/mL).…”
Section: Resultsmentioning
confidence: 99%
“…The specificity of MIP is often evaluated based on the partition coefficient, selectivity coefficient, and relative selectivity coefficient calculated by Eqs. ( 2 )–( 5 ): where IF is the imprinting factor, K d is the distribution coefficient, K is the selectivity coefficient of MIP and NIP for l -cysteine, K d1 is the partition coefficient of l -cysteine, K d2 is the partition coefficient of competitors, and K ʹ is the relative selectivity coefficient 26 .…”
In this work, we explored a new approach to a simple and sensitive fluorescence detection of thiols. The approach takes advantage of an in-situ formation of UV light-induced fluorescent nanoparticles (ZnCd/S quantum dots), while utilizing the thiol group of the analyte as a capping agent. The selectivity is ensured by the selective isolation of the thiol analyte by a polydopamine molecularly imprinted polymeric (MIP) layer. Based on this approach, a method for determination of thiols was designed. Key experimental parameters were optimized, including those of molecular imprinting and of effective model thiol molecule (l-cysteine) isolation. The relationship between the fluorescence intensity of ZnCd/S quantum dots and the concentration of l-cysteine in the range of 12–150 µg/mL was linear with a detection limit of 3.6 µg/mL. The molecularly imprinted polymer showed high absorption mass capacity (1.73 mg/g) and an excellent selectivity factor for l-cysteine compared to N-acetyl-l-cysteine and l-homocysteine of 63.56 and 87.48, respectively. The proposed method was applied for l-cysteine determination in human urine with satisfactory results. Due to a high variability of molecular imprinting technology and versatility of in-situ probe formation, methods based on this approach can be easily adopted for analysis of any thiol of interest.
“…Variasi jumlah crosslinker dapat mempengaruhi selektivitas MIP yang ditunjukkan oleh nilai persen ekstraksi yang diperoleh [18]. Pengaruh jumlah crosslinker yang digunakan pada sintesis MIP juga dapat mempengaruhi nilai kemampuan adsorpsi MIP-CAP [19]. Kinerja adsorben MIP-CAP dapat dilihat dari nilai kemampuan adsorpsi, sehingga pada penelitian ini memiliki tujuan untuk mengetahui pengaruh jumlah crosslinker pada sintesis MIP terhadap kemampuan adsorpsi CAP dan persen ekstraksi dari variasi jumlah EGDMA, menganalisis serta membandingkan keberadaan gugus NO2 pada polimer terbaik.…”
Penelitian ini bertujuan untuk mengetahui pengaruh variasi jumlah crosslinker terhadap kemampuan adsorpsi Molecularly Imprinted Polymer (MIP)-Kloramfenikol (CAP) yang dianalisis menggunakan instrumen High-Performance Liquid Chromatography (HPLC) dan mengidentifikasi gugus fungsional –NO2 pada polimer dengan variasi jumlah crosslinker terbaik menggunakaan instrumen Fourier Transform Infrared Spectroscopy (FTIR). Dalam penelitian ini, MIP disintesis dengan menggunakan CAP sebagai template, etilen glikol dimetakrilat (EGDMA) sebagai crosslinker, asam metakrilat (MAA) sebagai monomer, benzoil peroksida (BPO) sebagai inisiator, dan asetonitril sebagai porogen. Hasil penelitian menunjukkan bahwa variasi jumlah crosslinker mempengaruhi kemampuan adsorpsi MIP dengan komposisi antara CAP: MAA: EGDMA yaitu 1:3:18 menghasilkan kemampuan adsorpsi terbesar yakni 2,2 mg/g dengan persen ekstraksi sebesar 66,11%. Hasil identifikasi gugus fungsional dengan FTIR menunjukkan bahwa NIP terdapat gugus –NO2 pada bilangan gelombang 1524,27 cm-1 sedangkan pada MIP tidak terdeteksi gugus –NO2 karena sebagian besar CAP telah berhasil diekstrak, serta PB yang dibuat sebagai polimer kontrol tanpa penambahan template CAP.
Kata kunci : Kloramfenikol, Molecularly Imprinted Polymer, Crosslinker, Kemampuan Adsorpsi
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