Recent update on methodologies for extraction and analysis of soybean seed proteins

Luthria, Devanand L.; John, K.M. Maria; Marupaka, Ramesh; Natarajan, Savithiry

doi:10.1002/jsfa.9235

Cited by 22 publications

(15 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Discovery proteomics is inherently hypothesis-generating, implementing relative or absolute quantification of individual proteins (protein dynamics), in response to internal or external factors [50]. Although to some extent, these tasks can be solved by other analytical techniques (e.g., immunochemical methods, like ELISA or Western blotting) [51], the most efficient way it can be achieved is by mass spectrometry coupled on-line with high-resolution separation techniques [52]. Thereby, protein identity can be assigned by tandem mass spectrometry (MS/MS), i.e., fragmentation of peptide ions, pre-selected in so-called full-MS survey scans (data-dependent acquisition, DDA) or acquired in parallel (data-independent acquisition, DIA) [53,54].…”

Section: Seed Proteomics: General Methodology and Applicationsmentioning

confidence: 99%

Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives

Smolikova

Gorbach

Lukasheva

et al. 2020

IJMS

View full text Add to dashboard Cite

For centuries, crop plants have represented the basis of the daily human diet. Among them, cereals and legumes, accumulating oils, proteins, and carbohydrates in their seeds, distinctly dominate modern agriculture, thus play an essential role in food industry and fuel production. Therefore, seeds of crop plants are intensively studied by food chemists, biologists, biochemists, and nutritional physiologists. Accordingly, seed development and germination as well as age- and stress-related alterations in seed vigor, longevity, nutritional value, and safety can be addressed by a broad panel of analytical, biochemical, and physiological methods. Currently, functional genomics is one of the most powerful tools, giving direct access to characteristic metabolic changes accompanying plant development, senescence, and response to biotic or abiotic stress. Among individual post-genomic methodological platforms, proteomics represents one of the most effective ones, giving access to cellular metabolism at the level of proteins. During the recent decades, multiple methodological advances were introduced in different branches of life science, although only some of them were established in seed proteomics so far. Therefore, here we discuss main methodological approaches already employed in seed proteomics, as well as those still waiting for implementation in this field of plant research, with a special emphasis on sample preparation, data acquisition, processing, and post-processing. Thereby, the overall goal of this review is to bring new methodologies emerging in different areas of proteomics research (clinical, food, ecological, microbial, and plant proteomics) to the broad society of seed biologists.

show abstract

Section: Seed Proteomics: General Methodology and Applicationsmentioning

confidence: 99%

Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives

Smolikova

Gorbach

Lukasheva

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Soybean seed proteins can be divided into structural proteins, metabolic proteins, and storage proteins according to their functionalities (Luthria, Maria John, Marupaka, & Natarajan, 2018). They could also be divided into 2S, 7S, 11S, and 15S according to their sedimentation coefficients (Bennetau‐Pelissero, 2019; Singh, Meena, Kumar, Dubey, & Hassan, 2015).…”

Section: Soybean Seedsmentioning

confidence: 99%

“…The five subunits are further divided into groups I and II according to their physical properties: group I includes G1, G2, and G3, whereas group II includes G4 (or IIb) and G5 (or IIa). Group I contain more methionine, an essential AA, than group II (Hsiao, Yu, Li, & Hsieh, 2015; Luthria et al., 2018). Moreover, from the molecular microstructure point of view, the 7S contains two sulfur–sulfur bonds and no sulfhydryl group.…”

Section: Soybean Seedsmentioning

confidence: 99%

Tofu products: A review of their raw materials, processing conditions, and packaging

Regenstein

Teng

et al. 2020

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Tofu is a traditional product made mainly from soybeans, which has become globally popular because of its inclusion in vegetarian, vegan, and hypocaloric diets. However, with both commercial production of tofu and scientific research, it remains a challenge to produce tofu with high quality, high nutrition, and excellent flavor. This is because tofu production involves multiple complicated steps, such as soybean selection, utilization of appropriate coagulants, and tofu packaging. To make high-quality tofu product, it is important to systematically understand critical factors that influence tofu quality. This article reviews the current research status of tofu production. The diversity of soybean seeds (the raw material), protein composition, structural properties, and nutritional values are reviewed. Then, selection of tofu coagulants is reviewed to provide insights on its role in tofu quality, where the focus is on the usage of mix coagulants and recent developments with new coagulants. Moreover, a comprehensive summary is provided on recent development in making high-fiber tofu using Okara (the major by-product during tofu production), which has a number of potential applications in the food industry. To help encourage automatic, environmental friendly, and high-efficient tofu production, new developments and applications in production technology, such as ultrasound and high-pressure process, are reviewed. Tofu packaging, including packaging materials and techniques, is evaluated as it has been found to have a positive impact on extending the shelf life and improving the quality of tofu products. Finally, the future research directions and potential areas for new developments are discussed.

show abstract

“…Over the decades, soybean (Glycine max (L) Merr) has been established as an important food commodity in Indonesia. It is the most important legume crop produced and consumed globally as an animal feed, cooking oil, and a component in many processed foods (Luthria et al 2018). Soybean has also been proposed as one of strategic food commodity by the Ministry of Agriculture, Republic of Indonesia, in addition to eleven other strategic food types, including rice, corn, shallots, garlic, large chilies, bird's eye chilies, beef/buffalo, purebred chicken, eggs, sugar, and cooking oil (Yohana and Bambang 2021).…”

Section: Introductionmentioning

confidence: 99%

“…cannot develop or reproduce if the plant does not produce seeds, presumably because seeds supply nutrients where the highest concentration of nutrients are contained in them compared to other parts of soybean plants (Mainali et al 2014). Soybean seeds have a relatively higher protein and lipid contents than other legumes (Luthria et al 2018;Min et al 2020;Huang et al 2019).…”

Section: Introductionmentioning

confidence: 99%

RESISTANT LEVEL OF SOYBEAN GERMPLASM AGAINST POD SUCKING BUGS (Riptortus spp.)

Herlina

Istiaji

Koswanudin

et al. 2021

Indones. J. Agric. Sci.

View full text Add to dashboard Cite

Increasing productivity of soybean has often been constrained by pod sucking bugs (Riptortus spp.) which caused a serious damage and yield losses up to 80%. Breeding for obtaining soybean variety resistant to pod suckers needs the availability of soybean germplasm resistant to the pest. The study aimed to obtain a candidate for soybean variety resistant to Riptortus spp. through the selection of 100 accessions of soybean. The study included the preparation of test plants and test insects, pest infestations, observations, and looking for a practical screening method for pod sucking pests. The experiment used a completely randomized design for two treatments (infested and non-infested Riptortus spp.). Cikuray variety and PI-092734 accession were used as a control. Results showed that there was a very low correlation among variables observed. Twelve soybean accessions showed a resistance to Riptortus spp., i.e. C7301-113AC-POP, Lokal Madiun-3549, Lokal Klungkung, ML.2974, Singgalang, Lokal Jepara, Lokal Jatim, Lokal Trenggalek, Lokal Tulungagung, Lokal Tabanan, Lokal Blitar, and Lokal Kuningan 10. These accessions were more resistant than the popular released variety such as Wilis, Grobogan, Detam 2, and Gepak Ijo. Small seed size was not a major determinant of soybean resistance to pod suckers. The addition of observational components, i.e. probing preference and oviposition, indicated that crop damage was indirectly influenced by the high frequency of probing and oviposition, although its relation to plant tolerant mechanisms still needs further investigation. Indeterminate plant types require further validation as to whether they contribute significantly to plant resistance against pod sucking insects.

show abstract

Recent update on methodologies for extraction and analysis of soybean seed proteins

Cited by 22 publications

References 84 publications

Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives

Bringing New Methods to the Seed Proteomics Platform: Challenges and Perspectives

Tofu products: A review of their raw materials, processing conditions, and packaging

RESISTANT LEVEL OF SOYBEAN GERMPLASM AGAINST POD SUCKING BUGS (Riptortus spp.)

Contact Info

Product

Resources

About