Recent trends in capillary electrophoresis for complex samples analysis: A review

Abstract

Recent trends in capillary electrophoresis for complex samples analysis: A reviewCE has been a continuously evolving analytical methodology since its first introduction in the 1980s of the last century. The development of new CE separation procedures, the coupling of these systems to more sensitive and versatile detection systems, and the advances in miniaturization technology have allowed the application of CE to the resolution of new and complex analytical problems, overcoming the traditional disadvantages associated with this method. In the present work, different recent trends in CE and their application to the determination of high complexity samples (as biological fluids, individual cells, etc.) will be reviewed: capillary modification by different types of coatings, microfluidic CE, and online microextraction CE. The main advantages and disadvantages of the different proposed approaches will be discussed with examples of most recent applications.

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (2015–mid 2018)

“…LC methods are currently preferred in proteomic or peptidomic studies but CE and microchip electrophoresis (MCE) techniques are also frequently employed in these fields as well as in the protein and peptide analyses in general [24,[45][46][47][48][49][50][51][52]. CE methods offer high resolution power, high separation efficiency, short analysis time, and require low amounts of samples and reagents [18].…”

Quantitative Analysis of Artificial Sweeteners by Capillary Electrophoresis with a Dual‐Capillary Design of Molecularly Imprinted Solid‐Phase Extractor

“…[1][2][3] The matrices of biological samples are complicated which can make noise or interferences during the analysis. CE allows high speed analysis, consumption of small sample volumes and does not need complex equipment.…”

Characterization of Athabasca lean oil sands and mixed surficial materials: Comparison of capillary electrophoresis/low‐resolution mass spectrometry and high‐resolution mass spectrometry

“…CE separates analytes dissolved in a liquid phase based on charge‐to‐size ratio and provides high separation capacity due to its nanoliter sample volumes and application of strong electric fields (30 kV). The coupling of CE to mass spectrometers via electrospray ionization (ESI) sources has made an impact in many fields of research . While high‐resolution mass spectrometers have provided the opportunity to unambiguously assign chemical formulae to m/z values, separation techniques such as CE, even when coupled to low‐resolution mass spectrometry (LRMS), could provide valuable complementary information regarding the components present in complex environmental mixtures.…”

“…The coupling of CE to mass spectrometers via electrospray ionization (ESI) sources has made an impact in many fields of research. [14][15][16][17] While high-resolution mass spectrometers have provided the opportunity to unambiguously assign chemical formulae to m/z values, separation techniques such as CE, even when coupled to low-resolution mass spectrometry (LRMS), could provide valuable complementary information regarding the components present in complex environmental mixtures.…”