Recent research and development of Antrodia cinnamomea

Proc. Natl. Acad. Sci. U.S.A.

et al. 2014

Self Cite

Antrodia cinnamomea, a polyporus mushroom of Taiwan, has long been used as a remedy for cancer, hypertension, and hangover, with an annual market of over $100 million (US) in Taiwan. We obtained a 32.15-Mb genome draft containing 9,254 genes. Genome ontology enrichment and pathway analyses shed light on sexual development and the biosynthesis of sesquiterpenoids, triterpenoids, ergostanes, antroquinonol, and antrocamphin. We identified genes differentially expressed between mycelium and fruiting body and 242 proteins in the mevalonate pathway, terpenoid pathways, cytochrome P450s, and polyketide synthases, which may contribute to the production of medicinal secondary metabolites. Genes of secondary metabolite biosynthetic pathways showed expression enrichment for tissuespecific compounds, including 14-α-demethylase (CYP51F1) in fruiting body for converting lanostane to ergostane triterpenoids, coenzymes Q (COQ) for antroquinonol biosynthesis in mycelium, and polyketide synthase for antrocamphin biosynthesis in fruiting body. Our data will be useful for developing a strategy to increase the production of useful metabolites.medicinal fungus | fruiting body | triterpenes | meiosis | P450

Section: Resultsmentioning

confidence: 99%

“…businesstoday.com.tw/article-content-80392-93594). For these reasons, numerous studies of its pharmacological properties have been conducted (12). Also, others have investigated the composition of polysaccharides and lipopolysaccharides and their differences between basidium and mycelium and among strains (11,13).…”

mentioning

confidence: 99%

Genomic and transcriptomic analyses of the medicinal fungusAntrodia cinnamomeafor its metabolite biosynthesis and sexual development

Fan

Proc. Natl. Acad. Sci. U.S.A.

et al. 2014

Self Cite

“…No metabolites with a retention time of 5.3 and 5.4 min appeared in the 72 h fermentation broth ( Figure S1). From the results, it is clear that antcin K was catalyzed by the strain to compound (1) and compound (2). Moreover, compound (1) and compound (2) were potentially biotransformed from 25S-antcin K and 25R-antcin K, respectively.…”

Section: Screening and Identification Of Soil Bacteria With Biotransfmentioning

confidence: 96%

“…This mushroom is endemic in Taiwan, and is used as a folk remedy in the treatment of a variety of diseases [2]. The fruiting bodies of A. cinnamomea contain abundant ergostane and lanostane tetracyclic triterpenoids, which are generally considered the major bioactive constituents of A. cinnamomea (Antrodia cinnamomea).…”

Section: Introductionmentioning

confidence: 99%

Biotransformation of Ergostane Triterpenoid Antcin K from Antrodia cinnamomea by Soil-Isolated Psychrobacillus sp. AK 1817

Chiang

et al. 2017

Catalysts

Antcin K is one of the major ergostane triterpenoids from the fruiting bodies of Antrodia cinnamomea, a parasitic fungus that grows only on the inner heartwood wall of the aromatic tree Cinnamomum kanehirai Hay (Lauraceae). To search for strains that have the ability to biotransform antcin K, a total of 4311 strains of soil bacteria were isolated, and their abilities to catalyze antcin K were determined by ultra-performance liquid chromatography analysis. One positive strain, AK 1817, was selected for functional studies. The strain was identified as Psychrobacillus sp., based on the DNA sequences of the 16S rRNA gene. The biotransformation metabolites were purified with the preparative high-performance liquid chromatography method and identified as antcamphin E and antcamphin F, respectively, based on the mass and nuclear magnetic resonance spectral data. The present study is the first to report the biotransformation of triterpenoids from A. cinnamomea (Antrodia cinnamomea).

“…Several mushrooms, such as Ganoderma lucidum, Hericium erinaceus and Antrodia cinnamomea, have historically been used in Asia for medicinal purposes [1,2]. Engleromyces goetzii, which belongs to the family Hypocreaceae, is a traditional medicinal mushroom that is primarily distributed in Tibet, Sichuan and Yunnan provinces.…”

Section: Introductionmentioning

confidence: 99%

Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii

Nat. Prod. Bioprospect.

Zhang

et al. 2015

Engleromyces goetzii is a traditional medicinal mushroom that is widely used to treat infection, inflammation and cancer in Tibet, Sichuan and Yunnan provinces of China. Two new trichothecenes, engleromycones A and B (1 and 2), one new cuparane-type sesquiterpenoid named infuscol F (11), eight known trichothecene analogs, sambucinol (3), 3-deoxysambucinol (4), trichothecolone (5), trichodermol (6), 8-deoxytrichothecin (7), trichothecin (8), trichothecinol B (9) and trichothecinol A (10), and one known cyclopentanoid sesquiterpene cyclonerodiol (12) were isolated from the cultures of E. goetzii. The new compounds were elucidated through spectroscopic analyses. The anticancer effects of trichothecenes 1-10 were examined in the HL-60, SMMC-7721, A549, MCF-7, and SW-480 human cancer cell lines using an MTT assay. Trichothecinol A (10) significantly inhibited the growth of MCF-7 cells, with an IC 50 value of 0.006 lM, which was comparable to the cytotoxic activity of the positive control, paclitaxel, indicating that trichothecinol A (10) represents a potential anticancer agent.