Recent progresses in gene delivery-based bone tissue engineering

Dual-controlled release system of drugs for bone regeneration

“…Gene therapy is an alternative therapeutic approach to bone regeneration, in which plasmid deoxyribonucleic acid (DNA), complementary DNA (cDNA), small interfering ribonucleic acid (siRNA), and micro RNA are used [97][98][99]. Plasmid DNA allows the local production of therapeutic protein by transfected cells.…”

Genetically Engineered Dental Stem Cells for Regenerative Medicine

“…Currently, research is focused on the use of MSCs for bone regeneration and stimulation of vascularization. For genetic modifi cation of the cells, scientists use genes encoding bone morphogenetic proteins (BMPs), vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1) and transforming growth factor-β (TGF-β) [ 12 ]. Kumar et al studied the effect of MSCs transduced with recombinant adenovirus encoding BMP2 and VEGF on the process of bone formation in a mouse model of segmental bone defect .…”

Repairing rabbit radial defects by combining bone marrow stroma stem cells with bone scaffold material comprising a core-cladding structure

“…A great deal of research has demonstrated that the extracellular matrix plays an important role in cell adhesion, proliferation, and osteogenic differentiation. Establishing extracellular matrices in vitro enables the cells to easily adhere to the surface of a scaffold material (Lu et al, 2013;Moshaverinia et al, 2013). Research has also demonstrated that introducing short peptides that promote cell adhesion, such as fibronectin, Asp-Gly-Glu-Ala (DGEA), and Arg-Gly-Asp (RGD) sequences, to the surface of the matrix lining by solvent linking or lining can effectively improve the biological activity of cells and promote their adhesion to the surface of the scaffold material (Asaoka et al, 2013).…”