Recent progress on rapid SARS-CoV-2/COVID-19 detection by CRISPR-Cas13-based platforms

Aquino‐Jarquín, Guillermo

doi:10.1016/j.drudis.2021.06.005

Cited by 20 publications

(21 citation statements)

References 44 publications

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“…Compared to the two-step assay, this single-step SHERLOCK assay could diagnose SARS-CoV-2 with reduced sample-to-answer time and equal sensitivity with optimized conditions [ 107 ]. Zhang et al combined RPA with SHERLOCK to detect the S gene and Orf1ab gene of SARS-COV-2 [ 108 ].…”

Section: Detection Mechanism Of Sars-cov-2mentioning

confidence: 99%

Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases

Wang

Dong

et al. 2022

Medicine in Novel Technology and Devices

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Corona Virus Disease 2019 (COVID-19) has developed into a global pandemic in the last two years, causing significant impacts on our daily life in many countries. Rapid and accurate detection of COVID-19 is of great importance to both treatments and pandemic management. Till now, a variety of point-of-care testing (POCT) approaches devices, including nucleic acid-based test and immunological detection, have been developed and some of them has been rapidly ruled out for clinical diagnosis of COVID-19 due to the requirement of mass testing. In this review, we provide a summary and commentary on the methods and biomedical devices innovated or renovated for the quick and early diagnosis of COVID-19. In particular, some of micro and nano devices with miniaturized structures, showing outstanding analytical performances such as ultra-sensitivity, rapidness, accuracy and low cost, are discussed in this paper. We also provide our insights on the further implementation of biomedical devices using advanced micro and nano technologies to meet the demand of point-of-care diagnosis and home testing to facilitate pandemic management. In general, our paper provides a comprehensive overview of the latest advances on the POCT device for diagnosis of COVID-19, which may provide insightful knowledge for researcher to further develop novel diagnostic technologies for rapid and on-site detection of pathogens including SARS-CoV-2.

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Section: Detection Mechanism Of Sars-cov-2mentioning

confidence: 99%

Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases

Wang

Dong

et al. 2022

Medicine in Novel Technology and Devices

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“…Recently, Tian et al described a droplet-digital Cas13a assay (ddCas13a) by harnessing the natural confinement effect, enhancing the local molecule concentration for highly efficient reaction or detection [44]. In order to potentiate the local concentration of target sequence and RNA reporter for the Cas13a system in cell-sized volumes, the authors employed a simple droplet microfluidic platform [44,45]. In this context, a mixture of target RNAs and Cas13a mixture is emulsified with oil into thousands of picoliter-sized droplet reactors.…”

Section: Ddcas13a Assay For Direct Single-molecule Microrna Quantificationmentioning

confidence: 99%

“…In this context, a mixture of target RNAs and Cas13a mixture is emulsified with oil into thousands of picoliter-sized droplet reactors. The accumulated fluorescent signal from the collateral cleavage of a single RNA target-activated Cas13a is sufficient to illuminate a picoliter-sized droplet [44,45] (Figure 2). In these conditions, after recognition by a crRNA, a single-target RNA can induce cleavage of over 10 4 quenched fluorescent RNA reporters, thus resulting in a fluorescently positive droplet.…”

Section: Ddcas13a Assay For Direct Single-molecule Microrna Quantificationmentioning

confidence: 99%

“…In these conditions, after recognition by a crRNA, a single-target RNA can induce cleavage of over 10 4 quenched fluorescent RNA reporters, thus resulting in a fluorescently positive droplet. Consequently, ddCas13a allows absolute digital quantitation (according to the Poisson distribution) of single unlabeled RNA molecules without the need for reverse transcription and amplification [44,45].…”

Section: Ddcas13a Assay For Direct Single-molecule Microrna Quantificationmentioning

confidence: 99%

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CRISPR/Cas13-Based Approaches for Ultrasensitive and Specific Detection of microRNAs

Granados-Riverón

Aquino‐Jarquín

2021

Cells

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MicroRNAs (miRNAs) have a prominent role in virtually every aspect of cell biology. Due to the small size of mature miRNAs, the high degree of similarity between miRNA family members, and the low abundance of miRNAs in body fluids, miRNA expression profiling is technically challenging. Biosensors based on electrochemical detection for nucleic acids are a novel category of inexpensive and very sensitive diagnostic tools. On the other hand, after recognizing the target sequence, specific CRISPR-associated proteins, including orthologues of Cas12, Cas13, and Cas14, exhibit collateral nonspecific catalytic activities that can be employed for specific and ultrasensitive nucleic acid detection from clinically relevant samples. Recently, several platforms have been developed, connecting the benefits of enzyme-assisted signal amplification and enzyme-free amplification biosensing technologies with CRISPR-based approaches for miRNA detection. Together, they provide high sensitivity, precision, and fewer limitations in diagnosis through efficient sensors at a low cost and a simple miniaturized readout. This review provides an overview of several CRISPR-based biosensing platforms that have been developed and successfully applied for ultrasensitive and specific miRNA detection.

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“…Application of rapid antigen testing, such as the current POCT of IgM/IgG and the ELISA technology, which targets viral antigens or antibodies, may provide fast and costeffective detection of HCoVs mostly at the early stage of infection. Zhang and colleagues recently developed a new diagnostic procedure called STOP (SHERLOCK Testing in One Pot) for SARS-CoV-2 rapid detection within 1 h [116]. Although the technology is still awaiting approval for clinical use, it is expected to remedy the high demand for a rapid test due to the rapidly increasing global spread of SARS-CoV-2.…”

Section: Clinical Diagnosismentioning

confidence: 99%

An Overview of the Pathogenesis, Transmission, Diagnosis, and Management of Endemic Human Coronaviruses: A Reflection on the Past and Present Episodes and Possible Future Outbreaks

et al. 2021

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The outbreak of the 2019 coronavirus pandemic caught the world by surprise in late 2019 and has held it hostage for months with an increasing number of infections and deaths. Although coronavirus was first discovered in the 1960s and was known to cause respiratory infection in humans, no information was available about the epidemic pattern of the virus until the past two decades. This review addresses the pathogenesis, transmission dynamics, diagnosis, management strategies, the pattern of the past and present events, and the possibility of future outbreaks of the endemic human coronaviruses. Several studies have described bats as presumptive natural reservoirs of coronaviruses. In essence, the identification of a diverse group of similar SARS coronaviruses in bats suggests the possibility of a future epidemic due to severe acute respiratory syndrome (SARS-like) coronaviruses originating from different reservoir hosts. The study also identified a lack of vaccines to prevent human coronavirus infections in humans in the past, however, the recent breakthrough in vaccine discovery and approval for emergency use for the treatment of Severe Acute Respiratory Syndrome Coronavirus 2 is commendable. The high rates of genomic substitution and recombination due to errors in RNA replication and the potential for independent species crossing suggest the chances of an entirely new strain evolving. Therefore, rapid research efforts should be deployed for vaccination to combat the COVID-19 pandemic and prevent a possible future outbreak. More sensitization and enlightenment on the need to adopt good personal hygiene practices, social distancing, and scientific evaluation of existing medications with promising antiviral effects against SARS-CoV-2 is required. In addition, intensive investigations to unravel and validate the possible reservoirs, the intermediate host, as well as insight into the ability of the virus to break the species barrier are needed to prevent future viral spillover and possible outbreaks.

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Recent progress on rapid SARS-CoV-2/COVID-19 detection by CRISPR-Cas13-based platforms

Cited by 20 publications

References 44 publications

Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases

Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases

CRISPR/Cas13-Based Approaches for Ultrasensitive and Specific Detection of microRNAs

An Overview of the Pathogenesis, Transmission, Diagnosis, and Management of Endemic Human Coronaviruses: A Reflection on the Past and Present Episodes and Possible Future Outbreaks

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