Recent progress on microfluidic devices with incorporated 1D nanostructures for enhanced extracellular vesicle (EV) separation

Abstract

Yuting Xiong and Hanyue Kang have contributed equally to this work.

Enhanced immune capture of extracellular vesicles with gelatin nanoparticles and acoustic mixing

“…Various structures including columns and quasi-triangle configurations 35 were designed and manufactured in the microfluidic chip to enhance the yield of captured products like cells and EVs. 22 Thus, the device we designed to isolate vesicles relied on a combination of the micro pillar structure and acoustic excitation by a PZT transducer, as shown in Fig. S1 † and Fig.…”

“…Therefore, the combination of microfluidic techniques and immunoaffinity has shown significant potential for isolating EVs. 13,21,22 For instance, Kanwar et al reported a typical ExoChip for on-chip isolation of EVs based on a microfluidic device treated with biotinylated anti-CD63. 23 Subsequently, Zhao et al devised the microfluidic platform ExoSearch to enhance collisions of EVs and allow for the isolation of vesicles through a serpentine fluidic channel.…”

Microfluidic Strategies for Extracellular Vesicle Isolation: Towards Clinical Applications

“…The most common microstructures are ordered rows of pillars [112][113][114], herringbone patterns [108,111,[115][116][117][118], and properly shaped microposts [119,120]. Meanwhile, in the case of nanopatterning, nanorods, nanowires, and more complex 3D structures [121] are typically used. In 2010, a pioneering work by Chen et al described a way in which to modify PDMS microfluidic channels presenting herringbone grooves with specific surface treatment [122].…”

Recent advances in micro-/nanostructure array integrated microfluidic devices for efficient separation of circulating tumor cells

“… 72 When combined with microfluidic devices, the physical interaction of fluid dynamics with the micro-/nanostructure can improve CTC sorting efficiency. 73 In the following, we discussed the two major types of CTC separating mechanisms based on microrods, one is physical, and the other is chemical.…”