Recent Progress in the Correlative Structured Illumination Microscopy

Abstract

The super-resolution imaging technique of structured illumination microscopy (SIM) enables the mixing of high-frequency information into the optical transmission domain via light-source modulation, thus breaking the optical diffraction limit. Correlative SIM, which combines other techniques with SIM, offers more versatility or higher imaging resolution than traditional SIM. In this review, we first briefly introduce the imaging mechanism and development trends of conventional SIM. Then, the principles and recent developments of correlative SIM techniques are reviewed. Finally, the future development directions of SIM and its correlative microscopies are presented.

Highly Accurate Profiling of Exosome Phenotypes Using Super-resolution Tricolor Fluorescence Co-localization

“…For example, breast tumor has at least three subtypes classified according to the tumor cell molecular profiles. The surface proteins of exosomes are specific and can reflect the physiological information on the parental cells. , A variety of exosomal proteins have been used to identify the tumor origins or subtypes, as well as distinguish between indolent and high-risk lesions. − However, highly accurate technologies for profiling and classifying the phenotype of cancers based on exosomal-specific proteins are still lacking.…”