Recent Progress in Small-Molecule Near-IR Probes for Bioimaging

Li, Junbin; Liu, Hong-Wen; Fu, Ting; Wang, Ruowen; Zhang, Xiaobing; Tan, Weihong

doi:10.1016/j.trechm.2019.03.002

Cited by 149 publications

(70 citation statements)

References 50 publications

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“…Contrast agents emitting in the visible or NIR-I optical windows have been widely studied and characterized in the last decades, using well known and affordable silicon based detectors and spectrometers 26,27 . SWIR-emitting contrast agents still remain poorly evaluated due to the short list of candidates as well as the rarity of in vivo SWIR imaging systems in research laboratories.…”

Section: -Fluorescence Detectability Of Swir Contrast Agentsmentioning

confidence: 99%

Optimization of spatial resolution and scattering effects for biomedical fluorescence imaging by using sub‐regions of the shortwave infrared spectrum

Musnier

Henry

Vollaire

et al. 2020

Journal of Biophotonics

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We evaluated the impact of light-scattering effects on spatial resolution in different SWIR subregions by analyzing two SWIR emissive phantoms made of PDMS-gold nanoclusters (Au NCs) composite covered with mice skin, or capillary tubes filled with Au NCs or IRDye 800CW at different depth in intralipids and finally, after administration of the Au NCs intravenously in mice. Our findings highlighted the benefit of working at the highest tested spectral range of the SWIR region with a 50% enhancement of spatial resolution measured in artificial model when moving from NIR-II (1000-1300 nm) to NIR-IIa (1300-1450 nm) region, and a 25% reduction of the scattering from the skin determined by point spread function analysis from the NIR-II to NIR-IIb region (1500-1700 nm). We also confirmed that a series of Monte Carlo restoration of images significantly improved the spatial resolution in vivo in mice in deep tissues both in the NIR-II and NIR-IIa spectral windows.

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Section: -Fluorescence Detectability Of Swir Contrast Agentsmentioning

confidence: 99%

Optimization of spatial resolution and scattering effects for biomedical fluorescence imaging by using sub‐regions of the shortwave infrared spectrum

Musnier

Henry

Vollaire

et al. 2020

Journal of Biophotonics

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“…Antibodies conjugated to low molecular weight fluorescent dyes have been used for various bioimaging applications [8]. Despite their cost-effectiveness, and water solubility, organic dyes display a small Stokes shift, low photochemical stability and they undergo photobleaching [9,10]. Luminescence can also be achieved via intramolecular energy transfer between an organic ligand and lanthanide metal ions through chelation [11].…”

Section: Introductionmentioning

confidence: 99%

Luminescent gold nanoclusters for bioimaging applications

Nonappa¹

2020

Beilstein J. Nanotechnol.

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Luminescent nanomaterials have emerged as attractive candidates for sensing, catalysis and bioimaging applications in recent years. For practical use in bioimaging, nanomaterials with high photoluminescence, quantum yield, photostability and large Stokes shifts are needed. While offering high photoluminescence and quantum yield, semiconductor quantum dots suffer from toxicity and are susceptible to oxidation. In this context, atomically precise gold nanoclusters protected by thiol monolayers have emerged as a new class of luminescent nanomaterials. Low toxicity, bioavailability, photostability as well as tunable size, composition, and optoelectronic properties make them suitable for bioimaging and biosensing applications. In this review, an overview of the sensing of pathogens, and of in vitro and in vivo bioimaging using luminescent gold nanoclusters along with the limitations with selected examples are discussed.

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“…The activated probe HSDP binding to mtDNA afforded a deep-red fluorescence emission (λ em = 650 nm) in the NIR) window, thus it improved contrast and depth of tissue imaging. [50] Importantly, the log P values of HSDP and HSDPP were 1.270 and 1.269 separately in octanol/water system, which implied the potential HSDP and HSDPP for effectively crossing the blood-brain barrier (BBB) to achieve imaging in the brain region with the brain-targeted characteristic. Therefore, we assessed HSDPP as a NIR imaging probe for tracking the endogenous H 2 S upregulation "regulated" by mtDNA in mice brains.…”

Section: Resultsmentioning

confidence: 97%

An Activatable and Switchable Nanoaggregate Probe for Detecting H₂S and Its Application in Mice Brains

Chen

Guo

et al. 2020

Chemistry An Asian Journal

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Employing a sequentially activated probe design method, an activatable, switchable and dual-mode probe was designed. This nanoprobe, HSDPP, could be effectively activated by H 2 S to form H-type aggregates with green emission; subsequently, the aggregates could bind to mtDNA to form monomers and the emIssion color switched from green to deep-red. We exploited HSDPP to image exogenous and endogenous H 2 S in living cells. Of note, for the first time, this novel nanoprobe with an optimal partition coefficient value (LogP = 1.269) was successfully applied for tracking the endogenous H 2 S upregulation stimulated by cystathionase activator S-adenosyl-L-methionine (SAM) in mice brains. Finally, our work provides an invaluable chemical tool for probing endogenous H 2 S upregulation in vitro/vivo and, importantly, affords a prospective design strategy for developing switchable chemosensors to unveil the relationship between biomolecules and DNA in mitochondria in many promising areas.

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Recent Progress in Small-Molecule Near-IR Probes for Bioimaging

Cited by 149 publications

References 50 publications

Optimization of spatial resolution and scattering effects for biomedical fluorescence imaging by using sub‐regions of the shortwave infrared spectrum

Optimization of spatial resolution and scattering effects for biomedical fluorescence imaging by using sub‐regions of the shortwave infrared spectrum

Luminescent gold nanoclusters for bioimaging applications

An Activatable and Switchable Nanoaggregate Probe for Detecting H₂S and Its Application in Mice Brains

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Recent Progress in Small-Molecule Near-IR Probes for Bioimaging

Cited by 149 publications

References 50 publications

Optimization of spatial resolution and scattering effects for biomedical fluorescence imaging by using sub‐regions of the shortwave infrared spectrum

Optimization of spatial resolution and scattering effects for biomedical fluorescence imaging by using sub‐regions of the shortwave infrared spectrum

Luminescent gold nanoclusters for bioimaging applications

An Activatable and Switchable Nanoaggregate Probe for Detecting H2S and Its Application in Mice Brains

Contact Info

Product

Resources

About

An Activatable and Switchable Nanoaggregate Probe for Detecting H₂S and Its Application in Mice Brains