Recent HIV infection rates among HIV positive patients seeking voluntary counseling and testing centers in the metropolitan region of Recife – PE, Brazil

Cavalcanti, Ana Maria Salustiano; Brito, Ana Maria de; Salustiano, Daniela Medeiros; Lima, Kledoaldo; Silva, Sirleide Pereira da; Lacerda, Heloísa Ramos

doi:10.1016/s1413-8670(12)70298-2

Cited by 9 publications

(5 citation statements)

References 17 publications

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“…The proportion of BF1 variants described in this study is much greater than the rate reported in 169 individuals recently diagnosed as seropositive for HIV1 (2.8%) in the Metropolitan Region of Recife, Northeastern of Brazil [32] and in other study of 84 patients chronically infected with HIV (3.6%) naïve to antiretroviral treatment [33] . This difference is not surprising, because small fragments from different regions of HIV genomes were characterized in the previous studies while we used larger overlapped fragments to sequence the NFLGs, which is the preferred method for accurate characterization of HIV-1 isolates.…”

Section: Discussioncontrasting

confidence: 68%

Deep Sequencing of HIV-1 near Full-Length Proviral Genomes Identifies High Rates of BF1 Recombinants Including Two Novel Circulating Recombinant Forms (CRF) 70_BF1 and a Disseminating 71_BF1 among Blood Donors in Pernambuco, Brazil

et al. 2014

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BackgroundThe findings of frequent circulation of HIV-1 subclade F1 viruses and the scarcity of BF1 recombinant viruses based on pol subgenomic fragment sequencing among blood donors in Pernambuco (PE), Northeast of Brazil, were reported recently. Here, we aimed to determine whether the classification of these strains (n = 26) extends to the whole genome sequences.MethodsFive overlapping amplicons spanning the HIV near full-length genomes (NFLGs) were PCR amplified from peripheral blood mononuclear cells (PBMCs) of 26 blood donors. The amplicons were molecularly bar-coded, pooled, and sequenced by Illumina paired-end protocol. The prevalence of viral variants containing drug resistant mutations (DRMs) was compared between plasma and PBMCs.ResultsOf the 26 samples studied, 20 NFLGs and 4 partial fragments were de novo assembled into contiguous sequences and successfully subtyped. Two distinct BF1 recombinant profiles designated CRF70_BF1 and CRF71_BF1, with 4 samples in profile I and 11 in profile II were detected and thus constitute two novel recombinant forms circulating in PE. Evidence of dual infections was detected in four patients co-infected with distinct HIV-1 subtypes. According to our estimate, the new CRF71_BF1 accounts for 10% of the HIV-1 circulating strains among blood donors in PE. Discordant data between the plasma and PBMCs-virus were found in 15 of 24 donors. Six of these strains displayed major DRMs only in PBMCs and four of which had detectable DRMs changes at prevalence between 1-20% of the sequenced population.ConclusionsThe high percentage of the new RF71_BF1 and other BF1 recombinants found among blood donors in Pernambuco, coupled with high rates of transmitted DRMs and dual infections confirm the need for effective surveillance to monitor the prevalence and distribution of HIV variants in a variety of settings in Brazil.

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Section: Discussioncontrasting

confidence: 68%

Deep Sequencing of HIV-1 near Full-Length Proviral Genomes Identifies High Rates of BF1 Recombinants Including Two Novel Circulating Recombinant Forms (CRF) 70_BF1 and a Disseminating 71_BF1 among Blood Donors in Pernambuco, Brazil

et al. 2014

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“…In the present study, 11 of 41 HIV-1 seropositive individuals (27.5%) were classified as recent seroconverters, corresponding to an estimated incidence rate of 4.88%/year (95% CI 2.00%–7.76%), which can be considered quite high. Indeed, in a previous study conducted by our group we found an estimated incidence of 1.68%/year (95% CI 1.26%–2.10%) for people seeking HIV diagnosis at Voluntary and Counselling Centers of Rio de Janeiro [33]. However, when these individuals were categorized by risk behavior, the estimated incidence among MSM was 11-fold higher than among heterosexual men (11.96% year (95% CI 6.10–17.82) vs. 1.12%/year (95% CI 0.55–1.68).…”

Section: Resultsmentioning

confidence: 71%

“…However, when these individuals were categorized by risk behavior, the estimated incidence among MSM was 11-fold higher than among heterosexual men (11.96% year (95% CI 6.10–17.82) vs. 1.12%/year (95% CI 0.55–1.68). This suggests that preventive actions aimed at the MSM community are insufficient, and a priority must be given to efforts focused on this group [33]. Among our study subjects, 7 men were infected with HCV and 8 were infected with HTLV.…”

Section: Resultsmentioning

confidence: 94%

HIV-1, HBV, HCV, HTLV, HPV-16/18, and Treponema pallidum Infections in a Sample of Brazilian Men Who Have Sex with Men

Soares

Georg²,

Lampe³

et al. 2014

PLoS ONE

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BackgroundMen who have sex with men (MSM) are more vulnerable to blood-borne infections and/or sexually-transmitted infections (STI). This study was conducted to estimate the prevalences of mono and co-infections of HIV-1 and other blood-borne/STIs in a sample of MSM in Campinas, Brazil.MethodsResponding Driven Sampling (RDS) was used for recruitment of MSM. Serum samples collected from 558 MSM were analyzed for the presence of serological markers for HIV-1, HBV, HCV, HTLV, HPV-16/18, and T. pallidum infections.ResultsThe highest prevalences of infection in serum samples were found for HPV-16 and 18 (31.9% and 20.3%, respectively). Approximately 8% of the study population showed infection with HIV-1, and within that group, 27.5% had recently become infected with HIV-1. HBV infection and syphilis were detected in 11.4% and 10% of the study population, respectively, and the rates of HTLV and HCV infection were 1.5% and 1%, respectively. With the exception of HTLV, all other studied infections were usually found as co-infections rather then mono-infections. The rates of co-infection for HCV, HPV-18, and HIV-1 were the highest among the studied infections (100%, 83%, and 85%, respectively). Interestingly, HTLV infection was usually found as a mono-infection in the study group, whereas HCV was found only as a co-infection.ConclusionsThe present findings highlight the need to educate the MSM population concerning their risk for STIs infections and methods of prevention. Campaigns to encourage vaccination against HBV and HPV could decrease the rates of these infections in MSM.

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“…Pernambuco was the second most studied state in Northeast Brazil, with eight articles being published reporting a subtype B frequency range of 54.6% 14 to 81.8%, 31 followed by subtype F (frequency range of 18.2 31 ‐39.8% 14 ). According to our analysis, Pernambuco has a HIV‐1 subtype B frequency (62.8%) 31 .…”

Section: Discussionmentioning

confidence: 99%

HIV‐1 subtype frequency in Northeast Brazil: A systematic review and meta‐analysis

Costa

Rodrigues

Morais

et al. 2020

Journal of Medical Virology

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Information on human immunodeficiency virus (HIV) molecular epidemiology is required to verify HIV/AIDS (acquired immune deficiency syndrome) epidemic dynamics in different regions, as well as provide support for response to antiretroviral therapy, transmission of resistance mutations, disease progression, and viral spread. The aim of this study was to conduct a systematic review and meta‐analysis of the frequency of HIV‐1 subtypes in Northeast Brazil. Seventy‐six articles that refer to HIV‐1 and its subtypes in the Northeast Brazil and published between 1 January 1999 and 31 August 2019 were identified. We included 27 articles for the qualitative synthesis, thus analyzing results from 4466 patients and 4298 genomic sequences. The results showed that subtypes B, F, and C and recombinant BF were responsible for 76% (IC95%: 71‐80), 8% (IC95%: 5‐11), 2% (IC95%: 2‐3), and 7% (IC95%: 4‐12) infections, respectively. The highest proportion of subtype B infections (82.2%) was observed in Piauí, while the subtype F had a high frequency in Pernambuco (23.4%). Bahia presented 11.6% of the proportion of recombinant BF. In addition, several recombinants such as AG, BC, BCF, and BD have been identified in the region. This is the first systematic review and meta‐analysis on the HIV‐1 subtype distribution in Northeast Brazil and has shown a high circulating viral diversity. Although subtype B is predominant in Brazil, a large frequency of non‐B subtypes has also been found, which may have consequences for response to antiretroviral therapy, disease progression, and transmission. Thus, HIV molecular epidemiological data are essential for epidemic prevention and control strategies.

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Recent HIV infection rates among HIV positive patients seeking voluntary counseling and testing centers in the metropolitan region of Recife – PE, Brazil

Cited by 9 publications

References 17 publications

Deep Sequencing of HIV-1 near Full-Length Proviral Genomes Identifies High Rates of BF1 Recombinants Including Two Novel Circulating Recombinant Forms (CRF) 70_BF1 and a Disseminating 71_BF1 among Blood Donors in Pernambuco, Brazil

Deep Sequencing of HIV-1 near Full-Length Proviral Genomes Identifies High Rates of BF1 Recombinants Including Two Novel Circulating Recombinant Forms (CRF) 70_BF1 and a Disseminating 71_BF1 among Blood Donors in Pernambuco, Brazil

HIV-1, HBV, HCV, HTLV, HPV-16/18, and Treponema pallidum Infections in a Sample of Brazilian Men Who Have Sex with Men

HIV‐1 subtype frequency in Northeast Brazil: A systematic review and meta‐analysis

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