Recent Findings on the Isolation, Identification and Quantification of Bioactive Peptides

Okoye, Charles Obinwanne; Ezeorba, Timothy Prince Chidike; Okeke, Emmanuel Sunday; Okagu, Innocent Uzochukwu

doi:10.1016/j.afres.2022.100065

Cited by 18 publications

(6 citation statements)

References 156 publications

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“…It is interesting to highlight the wide biological properties that this group of bioactive peptides shows depending on the peptide studied, including antihypertensive, antioxidant, antiproliferative, anti-inflammatory, apiaceous, hypocholesteremia, antithrombotic and mineral chelator functions [ 40 , 41 , 42 , 43 , 44 , 53 ]. On the other hand, phenolic compounds, including flavonoids (anthocyanins, flavanols, catechins, gallo catechins, etc.…”

Section: Sources Of Nutraceuticals With Biological Valuementioning

confidence: 99%

“…Preliminary studies must be performed to study this factor with each compound and each solubility, taking into account their polarity, functional groups, molecular weight, if it is oxidized or reduced or if it is complexed to another molecule. Indeed, most of the bioactive compounds have low water solubility, including carotenoids, bioactive lipids and some phenolic compounds, whereas bioactive peptides are usually more hydrophilic [ 42 , 43 , 44 ]. Wall materials’ compatibility: in NLCs, liquid lipid and solid lipid molecules should have good miscibility and compatibility with each other.…”

Section: Challenges Encountered In Nutraceutical Stabilitymentioning

confidence: 99%

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Nutraceuticals and Food-Grade Lipid Nanoparticles: From Natural Sources to a Circular Bioeconomy Approach

Blanco-Llamero

Fonseca

Durazzo³

et al. 2022

Foods

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Nutraceuticals have gained increasing attention over the last years due to their potential value as therapeutic compounds formulated from natural sources. For instance, there is a wide range of literature about the cardioprotective properties of omega-3 lipids and the antioxidant value of some phenolic compounds, which are related to antitumoral activity. However, the value of nutraceuticals can be limited by their instability under gastric pH and intestinal fluids, their low solubility and absorption. That is why encapsulation is a crucial step in nutraceutical design. In fact, pharmaceutical nanotechnology improves nutraceutical stability and bioavailability through the design and production of efficient nanoparticles (NPs). Lipid nanoparticles protect the bioactive compounds from light and external damage, including the gastric and intestinal conditions, providing a retarded delivery in the target area and guaranteeing the expected therapeutic effect of the nutraceutical. This review will focus on the key aspects of the encapsulation of bioactive compounds into lipid nanoparticles, exploring the pharmaceutical production methods available for the synthesis of NPs containing nutraceuticals. Moreover, the most common nutraceuticals will be discussed, considering the bioactive compounds, their natural source and the described biological properties.

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Section: Sources Of Nutraceuticals With Biological Valuementioning

confidence: 99%

Section: Challenges Encountered In Nutraceutical Stabilitymentioning

confidence: 99%

Nutraceuticals and Food-Grade Lipid Nanoparticles: From Natural Sources to a Circular Bioeconomy Approach

Blanco-Llamero

Fonseca

Durazzo³

et al. 2022

Foods

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“…Recently, quantitative structure–activity relationship analysis was developed based on the principle of enzyme proteolysis and previous knowledge of amino acid residues in polypeptide chains to reduce the time and effort in identifying proteins and peptides with important bioactive properties such as angiotensin I‐converting enzyme‐inhibitory, anti‐inflammatory, antioxidant and osteoprotective properties (Barley et al, 2018; Deng et al, 2017; Fontaine et al, 2019; Mao et al, 2021). For general details on protein preparation, isolation, purification and quantification, see recent reviews (Okagu et al, 2022; Okoye et al, 2022). Peptides with known osteo‐modulatory activities can further be used for the development of functional food products and information on the peptides can be submitted to databases such as BIOPEP‐UWM, FepeptideDB, PlantPepDB and StraPep.…”

Section: Sources and Isolation Of Food Proteins And Peptides With Ost...mentioning

confidence: 99%

Osteo‐modulatory dietary proteins and peptides: A concise review

Okagu

Aham

Ezeorba

et al. 2022

Journal of Food Biochemistry

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“…To this end, it becomes imperative to know their distinctive functionalities by separating them from their natural matrices and carrying out their characterization. This separation brings about the unfolding of their functional groups, which interact with target tissues [6]. For scientists to have a deeper understanding of such (potential or already established) therapeutic candidates' biomolecular mechanisms and interactions, biophysical and biochemical characterization of specific quality and functions becomes imperative.…”

Section: Introductionmentioning

confidence: 99%

Principles of Biophysical and Biochemical Characterization of Root Vegetables’ Bioactive Proteins

Onisuru¹,

Onisuru²

2023

Advances in Root Vegetables Research

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The characterization of root vegetables’ bioactive proteins biophysically and biochemically becomes imperative as they play an incredibly important role in the discovery, development, and formulation of bioactive proteins as biopharmaceutical products. This is because bioactive proteins differ in terms of size, molecular weights, structures, and physicochemical properties. Biophysical and biochemical characterization employs several techniques ranging from simple to complex procedures to give insight into proteins’ high-order structures, functions, and biochemical activities. Owing to the increasing awareness and acceptance of the use of peptides and proteins of root vegetable origin as treatment agents against some debilitatingly chronic diseases, researchers are now exploring an eco-innovative approach to reduce their loss by getting to structurally and functionally characterizing them. Several biophysical and biochemical tools are employed routinely for protein characterization and some of which are ultraviolet-visual (UV-Vis) spectroscopy, high-performance liquid chromatography (HPLC), circular dichroism (CD), intrinsic tryptophan fluorescence (ITF), differential scanning calorimetry (DSC), thermal shift assay (TSA), among others.

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Recent Findings on the Isolation, Identification and Quantification of Bioactive Peptides

Cited by 18 publications

References 156 publications

Nutraceuticals and Food-Grade Lipid Nanoparticles: From Natural Sources to a Circular Bioeconomy Approach

Nutraceuticals and Food-Grade Lipid Nanoparticles: From Natural Sources to a Circular Bioeconomy Approach

Osteo‐modulatory dietary proteins and peptides: A concise review

Principles of Biophysical and Biochemical Characterization of Root Vegetables’ Bioactive Proteins

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