Recent developments in the optimization of thermostable DNA polymerases for efficient applications

Павлов, А. В.; Pavlova, Nadejda V.; Kozyavkin, Sergei A.; Slesarev, Alexei I.

doi:10.1016/j.tibtech.2004.02.011

Cited by 113 publications

(74 citation statements)

References 83 publications

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“…In this study, we used Taq DNA polymerase, an important enzyme in biotechnology (Dalton, 1999(Dalton, , 2001Pavlov et al, 2004), as a model substrate for examining the effects of chaperones on stability and durability of Taq polymerase, by incorporating chaperones into PCR reaction mixtures.…”

Section: Resultsmentioning

confidence: 99%

Stabilization of Taq DNA Polymerase at High Temperature by Protein Folding Pathways From a Hyperthermophilic Archaeon, Pyrococcus furiosus

Laksanalamai

Павлов

Slesarev

et al. 2005

Biotech & Bioengineering

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Pyrococcus furiosus, a hyperthermophilic archaeon growing optimally at 1008C, encodes three protein chaperones, a small heat shock protein (sHsp), a prefoldin (Pfd), and a chaperonin (Cpn). In this study, we report that the passive chaperones sHsp and Pfd from P. furiosus can boost the protein refolding activity of the ATP-dependent Cpn from the same hyperthermophile. The thermo-stability of Taq polymerase was significantly improved by combinations of P. furiosus chaperones, showing ongoing protein folding activity at elevated temperatures and during thermal cycling. Based on these results, we propose that the protein folding apparatus in the hyperthermophilic archaeon, P. furiosus can be utilized to enhance the durability and cost effectiveness of high temperature biocatalysts.

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Section: Resultsmentioning

confidence: 99%

Stabilization of Taq DNA Polymerase at High Temperature by Protein Folding Pathways From a Hyperthermophilic Archaeon, Pyrococcus furiosus

Laksanalamai

Павлов

Slesarev

et al. 2005

Biotech & Bioengineering

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“…Gram +ve cocci were cultured on Manitoal salt agar. The bacterial isolates were biochemically identified by the following tests Haemolysin production (Pavlov et al, 2004), Methyl red (MR) (MacFaddin, 1985) and VogesProskauer (VP) (MacFaddin,1980). Also, catalase (Koneman et al, 1992) test was performed to Gram +ve cocci.…”

Section: Bacterial Colony Count In Urine Samples Of Uti and Biochemicmentioning

confidence: 99%

Correlation between Biofilm Production and Bacterial Urinary Tract Infections: New Therapeutic Approach

Allam

2017

Egypt. J. Microbiol.

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“…The reactions were incubated at 37 C overnight before electrophoresis. Electrophoresis was carried out on a 3% agarose gel and visualized after staining with ethidium bromide [Pavlova et al 2004;Federici et al 2003]. …”

Section: Dna Genotypingmentioning

confidence: 99%

Effect of genetic polymorphisms on the development of secondary failure to sulfonylurea in Egyptian patients with type 2 diabetes

El‐Sisi

Hegazy

Metwally

et al. 2011

Therapeutic Advances in Endocrinology

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Abstract:Objective: This study investigated the possibility that genetic factors, such as polymorphism of K inward rectifier subunit (Kir6.2), E23K, and Arg 972 polymorphism of insulin receptor substrate-1 (IRS-1), may predispose patients to sulfonylurea failure. Methods: A total of 100 unrelated Egyptian patients with type 2 diabetes were recruited. They were divided into two equal groups: group I consisted of patients with secondary failure to sulfonylurea (hemoglobin A 1c ! 8% despite sulfonylurea therapy) while group II consisted of patients whose condition was controlled with oral therapy. Results: Of all the patients, 45% and 14% were carriers of the K allele and Arg 972 variants respectively. The frequency of the K allele was 34% among patients with diabetes that was controlled with oral therapy and 56% among patients with secondary failure to sulfonylurea. The frequency of the Arg 972 IRS-1 variant was 6% among patients with diabetes controlled with oral therapy and 22% among patients with secondary failure. Conclusion: The E23K variant of the Kir6.2 gene and Arg 972 IRS-1 variants are associated with increased risk for secondary failure to sulfonylurea.

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Recent developments in the optimization of thermostable DNA polymerases for efficient applications

Cited by 113 publications

References 83 publications

Stabilization of Taq DNA Polymerase at High Temperature by Protein Folding Pathways From a Hyperthermophilic Archaeon, Pyrococcus furiosus

Stabilization of Taq DNA Polymerase at High Temperature by Protein Folding Pathways From a Hyperthermophilic Archaeon, Pyrococcus furiosus

Correlation between Biofilm Production and Bacterial Urinary Tract Infections: New Therapeutic Approach

Effect of genetic polymorphisms on the development of secondary failure to sulfonylurea in Egyptian patients with type 2 diabetes

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