Recent developments in the methodology and application of MEEKC

Ryan, Richie; McEvoy, Eamon; Donegan, Sheila; Power, Joe; Altria, Kevin D.

doi:10.1002/elps.201000372

Cited by 36 publications

(18 citation statements)

References 98 publications

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“…The latest developments and applications of EKC are presented in the reviews on its micellar mode (MEKC) 117–121 and microemulsion mode (MEEKC) 122–124. MEKC with ionogenic detergents, typically anionic SDS or cationic CTAB or zwitterionic CHAPS, is particularly suitable for separation of electroneutral peptides, i.e.…”

Section: Separations In Different Ce and Cec Modesmentioning

confidence: 99%

Recent developments in CE and CEC of peptides (2009–2011)

Kašička

2011

Electrophoresis

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The review brings a comprehensive survey of the recent developments of high-performance electroseparation methods in capillary and microchip formats: zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography. Applications of these techniques to analysis, isolation, purification and physicochemical and biochemical characterization of peptides are described. Advances in the investigation of electromigration properties of peptides, and in the methodology of their analysis, such as sample preparation, adsorption suppression, EOF control and detection, are presented. New developments, in particular, CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated.

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Section: Separations In Different Ce and Cec Modesmentioning

confidence: 99%

Recent developments in CE and CEC of peptides (2009–2011)

Kašička

2011

Electrophoresis

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“…Microemulsions are stable, isotropically clear solutions consisting of nanometer‐size oil droplets suspended in aqueous buffer, stabilized by a surfactant and a cosurfactant 279. Cao et al conducted a series of experiments focusing on MEEKC analysis of phenolic acids and diterpenoids in Salvia miltiorrhiza 201, 202, 230, 254, saponins in Panax notoginseng 239, 241, and flavonoids in Radix Astragali.…”

Section: Separationmentioning

confidence: 99%

CE and CEC analysis of phytochemicals in herbal medicines

et al. 2011

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CE and CEC, due to their versatility and high efficiency, have attracted great interest in the analysis of phytochemicals in herbs and their preparations. Previously, we reviewed the analysis of phytochemical bioactive compounds by CE in 2006 (Electrophoresis 2006, 27, 4808-4819) or CEC in 2010 (Electrophoresis 2010, 31, 260-277). This review followed the previous studies and covered the literature published since 2006 for CE and 2009 for CEC (excluding those mentioned in the two previous reviews), which emphasized the development of CE and CEC techniques in phytochemical analysis. In addition, sample preparation and detection were also discussed.

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“…They are isotropically clear solutions consisting of a lipophilic component (e.g. octane) and water, and are stabilized by adding a surfactant or a co‐surfactant whose presence reduces the interfacial tension to almost zero . Today ME systems are widely applied in CE in a mode called MEEKC which utilizes MEs as the separation medium .…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a generic stability‐indicating MEEKC method for five fluoroquinolone antibiotics

2015

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In this paper, a reliable stability-indicating generic MEEKC method for the analysis of five commonly used fluoroquinolones (FQs) has been developed and optimized by a central composite circumscribed experimental design. The separation was carried out using a fused silica capillary (60.2 cm total length) and a microemulsion (ME) composed of 81.75% (w/w) of a 125 mM NaH PO solution having a pH of 2.75, 2.65% (w/w) SDS, 1.00% (w/w) n-octanol, 6.60% (w/w) n-butanol and 8.00% (w/w) 2-propanol. A voltage of 28 kV was applied in a reverse polarity mode. A linear relationship was established from 0.04 to 0.48 mg/ml with R values higher than 0.98 for all five FQs. Both repeatability and intermediate precision were less than 3% and accuracy ranging from 97 to 100%. Of note, ciprofloxacin impurity A and ofloxacin impurity A could be separated from the respective drug substance in a single run which cannot be achieved using the official HPLC method from the European Pharmacopoeia. Forced degradation of all FQs under heat, in acidic and alkaline medium, in the presence of oxidizing agents and under neutral hydrolysis conditions was investigated. The highest yield of degradation products was observed using oxidative hydrogen peroxide. Hence, the proposed MEEKC method can be used for the quantitative determination of the five FQs and their potential impurities within a total runtime of 20 min.

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Recent developments in the methodology and application of MEEKC

Cited by 36 publications

References 98 publications

Recent developments in CE and CEC of peptides (2009–2011)

Recent developments in CE and CEC of peptides (2009–2011)

CE and CEC analysis of phytochemicals in herbal medicines

Development and validation of a generic stability‐indicating MEEKC method for five fluoroquinolone antibiotics

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