Recent Developments in Polymer Microfluidic Devices with Capillary Electrophoresis and Electrochemical Detection

Abstract

We review recent work on the development of polymer microfluidic devices using capillary electrophoresis (CE) for fluid driving and separation and electrochemical detection (ED). A variety of commonly used off-chip and integrated electrochemical detection approaches, including amperometric, conductimetric and potentiometric detection approaches, have been given. We describe fabrication approaches for the creation of microfluidic architecture in a polymer substrate and electrode integration. The methods for polymer surface modification to enhance the microfluidic device properties are also described as well as developments in power supply for the creation of a robust CE-ED microfluidic device system. A variety of applications are described for the CE-ED systems.

Fabrication Methods for Microfluidic Devices: An Overview

“…There is increasing interest within wearable and flexible electronics for diagnostic applications [ 216 , 217 , 218 ]. There are a wide range of examples, including skin-based sensors for wireless physiological monitoring for neonate and pediatric care [ 219 ], flexible sensors for wound management [ 220 ], DNA analysis using melting curve analysis with an integrated thin film heater [ 221 ], and impedimetric point-of-care diagnostic for selected biomarkers [ 222 ].…”

Microfluidic Bioreactors for Cell Culturing: A Review

“…The microbioreactor environment was capable for mimicking the physiological liver mass transport and enabled the long term culture of hepatocytes cells without loss of viability. Microfabrication methods that are applicable for the creation of microfluidic devices are described in detail elsewhere [63,[165][166][167][168].…”

Microbioreactor for lower cost and faster optimisation of protein production

“…14 More generally, there is considerable potential for use of electrochemical detection within microfluidic microbioreactor systems. 15 Polydimethylsiloxane (PDMS) has a number of favourable properties for the development of microfluidic devices for cell culture including biocompatibility and transparency. 16 We have previously used PDMS for the integration of normally closed pneumatically actuated valves within a microfluidic device to allow sophisticated processing for culturing of cells for cytotoxicity assay.…”