Microbioreactor for lower cost and faster optimisation of protein production

Parekh, Mayur; ali, abdulaziz; Ali, Zulfiqur; Bateson, Simon; Abugchem, Fathi; Pybus, Leon P.; Lennon, Christopher

doi:10.1039/d0an01266a

Cited by 11 publications

(6 citation statements)

References 46 publications

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“…The particles (3D dots) are around 50 to 100 µm in diameter. We have previously described use of photopolymer inkjet 3D printing (i3DP) for creation of a microbioreactor with different functional layers joined by structured adhesive film [145] as well as fast and low cost prototyping of the mechanical and structural elements for a highly sensitive optical cavity enhanced absorption (CEA) detection system [146] (Figure 5). Vat polymerisation-comprising stereolithography (SLA) and digital light processing (DLP)-are amongst the most widely used 3D printing approaches and use UV to cure resin and build a 3D printed object, essentially involving photochemical synthesis of materials.…”

Section: D Printingmentioning

confidence: 99%

Fabrication Methods for Microfluidic Devices: An Overview

2021

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Microfluidic devices offer the potential to automate a wide variety of chemical and biological operations that are applicable for diagnostic and therapeutic operations with higher efficiency as well as higher repeatability and reproducibility. Polymer based microfluidic devices offer particular advantages including those of cost and biocompatibility. Here, we describe direct and replication approaches for manufacturing of polymer microfluidic devices. Replications approaches require fabrication of mould or master and we describe different methods of mould manufacture, including mechanical (micro-cutting; ultrasonic machining), energy-assisted methods (electrodischarge machining, micro-electrochemical machining, laser ablation, electron beam machining, focused ion beam (FIB) machining), traditional micro-electromechanical systems (MEMS) processes, as well as mould fabrication approaches for curved surfaces. The approaches for microfluidic device fabrications are described in terms of low volume production (casting, lamination, laser ablation, 3D printing) and high-volume production (hot embossing, injection moulding, and film or sheet operations).

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Section: D Printingmentioning

confidence: 99%

Fabrication Methods for Microfluidic Devices: An Overview

2021

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“… 107 , 108 , 109 The safety incredulity of gene therapy is even worsening recently due to the newly discovered polymerase θ , a unique DNA polymerase‐helicase fusion protein that can convert RNA segments back into DNA in mammalian cells. 110 , 111 Given the fast development of low‐cost protein production, 112 , 113 using the protein form of the therapeutic agent, such as in the case of FMOD—a native ECM molecule broadly distributed in connective tissues, 114 , 115 may be much safer and more financially efficient for human usage.…”

Section: Discussionmentioning

confidence: 99%

A novel injectable fibromodulin‐releasing granular hydrogel for tendon healing and functional recovery

Xue

Chen

et al. 2022

Bioengineering & Transla Med

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A crucial component of the musculoskeletal system, the tendon is one of the most commonly injured tissues in the body. In severe cases, the ruptured tendon leads to permanent dysfunction. Although many efforts have been devoted to seeking a safe and efficient treatment for enhancing tendon healing, currently existing treatments have not yet achieved a major clinical improvement. Here, an injectable granular hyaluronic acid (gHA)‐hydrogel is engineered to deliver fibromodulin (FMOD)—a bioactive extracellular matrix (ECM) that enhances tenocyte mobility and optimizes the surrounding ECM assembly for tendon healing. The FMOD‐releasing granular HA (FMOD/gHA)‐hydrogel exhibits unique characteristics that are desired for both patients and health providers, such as permitting a microinvasive application and displaying a burst‐to‐sustained two‐phase release of FMOD, which leads to a prompt FMOD delivery followed by a constant dose‐maintaining period. Importantly, the generated FMOD‐releasing granular HA hydrogel significantly augmented tendon‐healing in a fully‐ruptured rat's Achilles tendon model histologically, mechanically, and functionally. Particularly, the breaking strength of the wounded tendon and the gait performance of treated rats returns to the same normal level as the healthy controls. In summary, a novel effective FMOD/gHA‐hydrogel is developed in response to the urgent demand for promoting tendon healing.

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“…The device, designed for biological applications, consists of two vertically stacked U-shaped channels, which share a central segment obtained by a multistep optical lithography process including a sacrificial layer modified by chemical reactions [40]. Motivated by further improving multilayer stability and fabrication processes and using materials suitable also for industrial exploitation [41], herein we demonstrate and test a novel, reusable 3D microfluidic device, in which the key characteristic is the inclusion of microfabricated PMMA substrates to obtain a structure that can work as an efficient micromixer or a gradient maker tool for LOC integration. The selection of PMMA as a rough material for LOC development derives from its suitability for milling molding and for biological applications, due to its mechanical and chemical characteristics, e.g., good thermal stability, transparency, chemical inertness, and biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

Innovative 3D Microfluidic Tools for On-Chip Fluids and Particles Manipulation: From Design to Experimental Validation

et al. 2021

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Micromixers are essential components in lab-on-a-chip devices, of which the low efficiency can limit many bio-application studies. Effective mixing with automation capabilities is still a crucial requirement. In this paper, we present a method to fabricate a three-dimensional (3D) poly(methyl methacrylate) (PMMA) fluidic mixer by combining computer-aided design (CAD), micromilling technology, and experimental application via manipulating fluids and nanoparticles. The entire platform consists of three microfabricated layers with a bottom reservoir-shaped microchannel, a central serpentine channel, and a through-hole for interconnection and an upper layer containing inlets and outlet. The sealing process of the three layers and the high-precision and customizable methods used for fabrication ensure the realization of the monolithic 3D architecture. This provides buried running channels able to perform passive chaotic mixing and dilution functions, thanks to a portion of the pathway in common between the reservoir and serpentine layers. The possibility to plug-and-play micropumping systems allows us to easily demonstrate the feasibility and working features of our device for tracking the mixing and dilution performances of the micromixer by using colored fluids and fluorescent nanoparticles as the proof of concept. Exploiting the good transparency of the PMMA, spatial liquid composition and better control over reaction variables are possible, and the real-time monitoring of experiments under a fluorescence microscope is also allowed. The tools shown in this paper are easily integrable in more complex lab-on-chip platforms.

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Microbioreactor for lower cost and faster optimisation of protein production

Abstract: Optimisation of bioprocesses relies on approaches that are either labour intensive or require expensive robotic systems. There is a need for fluidic processing at low volume that can be integrated...

Cited by 11 publications

References 46 publications

Fabrication Methods for Microfluidic Devices: An Overview

Fabrication Methods for Microfluidic Devices: An Overview

A novel injectable fibromodulin‐releasing granular hydrogel for tendon healing and functional recovery

Innovative 3D Microfluidic Tools for On-Chip Fluids and Particles Manipulation: From Design to Experimental Validation

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