Recent Developments in Microfluidic Chip-Based Separation Devices Coupled to MS for Bioanalysis

Abstract

In recent years, the development of microfluidic chip separation devices coupled to MS has dramatically increased for high-throughput bioanalysis. In this review, advances in different types of microfluidic chip separation devices, such as electrophoresis- and LC-based microchips, as well as 2D design of microfluidic chip-based separation devices will be discussed. In addition, the utilization of chip-based separation devices coupled to MS for analyzing peptides/proteins, glycans, drug metabolites and biomarkers for various bioanalytical applications will be evaluated.

Recent advances in combination of capillary electrophoresis with mass spectrometry: Methodology and theory

“…Applications of microfluidic separation devices coupled to MS have dramatically increased the throughput in bioanalysis . Microfluidic devices enable an integration of proteomic workflows such as sample handling, sample treatment and transfer steps , and combination of separation steps with ESI or MALDI for applications in proteomics and cell analysis .…”

Analysis of proteins and peptides by electromigration methods in microchips

“…To spray the effluent from CE channel, either an external emitter is attached to the end of a channel, or an edge or a corner of the microchip is shaped into a nanospray emitter. Reviews of chip‐based devices coupled to MS detection and other MCE–MS and CE–MS systems have been recently published.…”

Repetitive injection method: A tool for investigation of injection zone formation and its compression in microfluidic liquid chromatography

“…Microfluidic columns with I.D. 's ranging from 0.075 to 0.5 mm are now commercially available for multiple applications, including proteomics, pharmaceutical analysis, and environmental analysis [1][2][3][4][5][6][7].…”