Recent advances on the spectroscopic characterization of microbial biofilms: A critical review

Sportelli, Maria Chiara; Kranz, Christine; Mizaikoff, Boris; Cioffi, Nicola

doi:10.1016/j.aca.2022.339433

Cited by 26 publications

(12 citation statements)

References 137 publications

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“…The results demonstrated both nanoparticle concentration and incubation time to be closely related to the toxicity of silver particles [Cui et al, 2015]. A fundamental understanding of the origin of the Raman signal and the correlation between the SERS signals from single cells and their metabolic activity is essential for reliable analysis [Sportelli et al, 2022]. An analysis of six different prokaryotic species (one archaeon, two Gram-positive bacteria, and three Gram-negative bacteria) demonstrated that the metabolic activity of the studied cells has a significant impact on the corresponding SERS signal at the single-cell level [Weiss et al, 2019].…”

Section: Raman Spectroscopymentioning

confidence: 96%

“…Studies have been performed on the analysis of pathogenic and nonpathogenic microorganisms using label-free SERS methods [Bodelón et al, 2018;Akanny et al, 2020;Liu et al, 2021]. It is noteworthy that SERS signals still strongly rely on the operational conditions related to sample preparation (i.e., nanoparticle morphology, chemical composition, concentration as well as the chemical nature of the SERS substrate) [Witkowska et al, 2019;Sportelli et al, 2022]. In addition, some metals, especially silver, show antibacterial effects and might affect microorganisms' viability.…”

Section: Raman Spectroscopymentioning

confidence: 99%

See 1 more Smart Citation

Targeted Cell Labeling and Sorting of Prokaryotes for Cultivation and Omics Approaches

Sturm,

Mojarrad,

Kaster

2023

Microb Physiol

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To date, the vast majority of prokaryotic organisms escapes detailed characterization because they cannot be isolated in axenic cultures. These organisms are referred to as microbial dark matter. Targeted labelling and sorting of these microorganisms pave the way for single-cell, enrichment, or cultivation approaches. In this review, we describe an array of different methods ranging from labeling-free to specific labelling techniques. In addition, different cell sorting methods and their combinations with targeting strategies are summarized and downstream applications like sequencing and cultivation are reviewed. Recent advances, challenges, and limitations of the particular methods are discussed with respect to cell viability, genome integrity as well as throughput, in order to help researchers select the most suitable methods for their specific research questions.

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Section: Raman Spectroscopymentioning

confidence: 96%

Section: Raman Spectroscopymentioning

confidence: 99%

Targeted Cell Labeling and Sorting of Prokaryotes for Cultivation and Omics Approaches

Sturm,

Mojarrad,

Kaster

2023

Microb Physiol

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“…The morphological assessment of biofilms is carried out using either spectroscopic- or microscopic-based techniques [ 94 , 95 ]. The advancement in these methods has also enabled the detection of the biofilms’ components, such as lipids, proteins, extracellular DNA, and humic substances [ 96 ].…”

Section: Biofilm Structural Analysis In Biohydrogen Reactorsmentioning

confidence: 99%

Elucidating the Role of Biofilm-Forming Microbial Communities in Fermentative Biohydrogen Process: An Overview

et al. 2022

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Amongst the biofuels described in the literature, biohydrogen has gained heightened attention over the past decade due to its remarkable properties. Biohydrogen is a renewable form of H2 that can be produced under ambient conditions and at a low cost from biomass residues. Innovative approaches are continuously being applied to overcome the low process yields and pave the way for its scalability. Since the process primarily depends on the biohydrogen-producing bacteria, there is a need to acquire in-depth knowledge about the ecology of the various assemblages participating in the process, establishing effective bioaugmentation methods. This work provides an overview of the biofilm-forming communities during H2 production by mixed cultures and the synergistic associations established by certain species during H2 production. The strategies that enhance the growth of biofilms within the H2 reactors are also discussed. A short section is also included, explaining techniques used for examining and studying these biofilm structures. The work concludes with some suggestions that could lead to breakthroughs in this area of research.

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“…They deal with bacterial biofilm infections involving many clinically relevant microorganisms [ 32 ] and consider different spectroscopic techniques (including a ca. 2-page-long part on FTIR spectroscopy) used to characterise microbial biofilms [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Fourier Transform Infrared (FTIR) Spectroscopic Study of Biofilms Formed by the Rhizobacterium Azospirillum baldaniorum Sp245: Aspects of Methodology and Matrix Composition

et al. 2023

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Biofilms represent the main mode of existence of bacteria and play very significant roles in many industrial, medical and agricultural fields. Analysis of biofilms is a challenging task owing to their sophisticated composition, heterogeneity and variability. In this study, biofilms formed by the rhizobacterium Azospirillum baldaniorum (strain Sp245), isolated biofilm matrix and its macrocomponents have for the first time been studied in detail, using Fourier transform infrared (FTIR) spectroscopy, with a special emphasis on the methodology. The accompanying novel data of comparative chemical analyses of the biofilm matrix, its fractions and lipopolysaccharide isolated from the outer membrane of the cells of this strain, as well as their electrophoretic analyses (SDS-PAGE) have been found to be in good agreement with the FTIR spectroscopic results.

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Recent advances on the spectroscopic characterization of microbial biofilms: A critical review

Cited by 26 publications

References 137 publications

Targeted Cell Labeling and Sorting of Prokaryotes for Cultivation and Omics Approaches

Targeted Cell Labeling and Sorting of Prokaryotes for Cultivation and Omics Approaches

Elucidating the Role of Biofilm-Forming Microbial Communities in Fermentative Biohydrogen Process: An Overview

Fourier Transform Infrared (FTIR) Spectroscopic Study of Biofilms Formed by the Rhizobacterium Azospirillum baldaniorum Sp245: Aspects of Methodology and Matrix Composition

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