Recent advances on application of peptide nucleic acids as a bioreceptor in biosensors development

Saadati, Arezoo; Hassanpour, Soodabeh; Guárdia, Miguel de la; Mosafer, Jafar; Hashemzaei, Mahmoud; Mokhtarzadeh, Ahad; Baradaran, Behzad

doi:10.1016/j.trac.2019.02.030

Cited by 94 publications

(45 citation statements)

References 100 publications

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“…Hence, their sensing performance may vary according to the solution used ( Afzal et al, 2017 ). A more stable alternative to DNA is the peptide nucleic acid (PNA), which is a DNA mimic produced by replacing the sugar-phosphate backbone with synthetic peptide bonds ( Saadati et al, 2019 ). In recent years, novel bio-recognition elements known as aptamers have been increasingly popular in replacing antibodies as the receptors in biosensors.…”

Section: Receptors For Target Recognitionmentioning

confidence: 99%

Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases

Lim

Saha

Tey

et al. 2020

Biosensors and Bioelectronics

130

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Infectious diseases are the ever-present threats to public health and the global economy. Accurate and timely diagnosis is crucial to impede the progression of a disease and break the chain of transmission. Conventional diagnostic techniques are typically time-consuming and costly, making them inefficient for early diagnosis of infections and inconvenient for use at the point of care. Developments of sensitive, rapid, and affordable diagnostic methods are necessary to improve the clinical management of infectious diseases. Quartz crystal microbalance (QCM) systems have emerged as a robust biosensing platform due to their label-free mechanism, which allows the detection and quantification of a wide range of biomolecules. The high sensitivity and short detection time offered by QCM-based biosensors are attractive for the early detection of infections and the routine monitoring of disease progression. Herein, the strategies employed in QCM-based biosensors for the detection of infectious diseases are extensively reviewed, with a focus on prevalent diseases for which improved diagnostic techniques are in high demand. The challenges to the clinical application of QCM-based biosensors are highlighted, along with an outline of the future scope of research in QCM-based diagnostics.

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Section: Receptors For Target Recognitionmentioning

confidence: 99%

Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases

Lim

Saha

Tey

et al. 2020

Biosensors and Bioelectronics

130

View full text Add to dashboard Cite

show abstract

“…And on the other hand, despite a significant structural change compared to natural DNA or other DNA analogs, PNAs are still capable of recognizing and binding specific DNA or RNA sequences following the Watson-Crick hydrogen bonding principle ( Egholm et al, 1993 ). Furthermore, owing to the absence of phosphate groups in the PNAs backbone, the combination of PNA to DNA (or RNA) will not be affected by electrostatic repulsion, which makes the DNA/PNA (or RNA/PNA) hybrids present more remarkable thermal stability and distinct ion strength effects than the ordinary double-stranded structure ( Saadati et al, 2019 ).…”

Section: Graphene Biosensors For Pathogensmentioning

confidence: 99%

Graphene biosensors for bacterial and viral pathogens

Jiang

Feng

et al. 2020

Biosensors and Bioelectronics

124

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: The infection and spread of pathogens (e.g., COVID-19) pose an enormous threat to the safety of human beings and animals all over the world. The rapid and accurate monitoring and determination of pathogens are of great significance to clinical diagnosis, food safety and environmental evaluation. In recent years, with the evolution of nanotechnology, nano-sized graphene and graphene derivatives have been frequently introduced into the construction of biosensors due to their unique physicochemical properties and biocompatibility. The combination of biomolecules with specific recognition capabilities and graphene materials provides a promising strategy to construct more stable and sensitive biosensors for the detection of pathogens. This review tracks the development of graphene biosensors for the detection of bacterial and viral pathogens, mainly including the preparation of graphene biosensors and their working mechanism. The challenges involved in this field have been discussed, and the perspective for further development has been put forward, aiming to promote the development of pathogens sensing and the contribution to epidemic prevention.

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“…The application of nucleic acids as a bioreceptor manages analysis of RNA/DNA-based biosensors. Moreover, nucleic acid bioreceptors are also employed for detection of pathogens regards to complementary base pairs [110].…”

Section: Substratesmentioning

confidence: 99%