Recent advances in surface plasmon resonance imaging: detection speed, sensitivity, and portability

Abstract

Surface plasmon resonance (SPR) biosensor is a powerful tool for studying the kinetics of biomolecular interactions because they offer unique real-time and label-free measurement capabilities with high detection sensitivity. In the past two decades, SPR technology has been successfully commercialized and its performance has continuously been improved with lots of engineering efforts. In this review, we describe the recent advances in SPR technologies. The developments of SPR technologies focusing on detection speed, sensitivity, and portability are discussed in details. The incorporation of imaging techniques into SPR sensing is emphasized. In addition, our SPR imaging biosensors based on the scanning of wavelength by a solid-state tunable wavelength filter are highlighted. Finally, significant advances of the vast developments in nanotechnology-associated SPR sensing for sensitivity enhancements are also reviewed. It is hoped that this review will provide some insights for researchers who are interested in SPR sensing, and help them develop SPR sensors with better sensitivity and higher throughput.

Development of automated angle-scanning, high-speed surface plasmon resonance imaging and SPRi visualization for the study of dropwise condensation

“…The conventional AM approach uses a goniometer with a manual rotating stage (Zeng et al 2017). This technique suffers from three main issues: low precision in angle probing, slow angle-scanning, and angle-dependent image artifacts.…”

Antimicrobial Susceptibility Test for the Determination of Resistant and Susceptible S. aureus and Enterococcus spp. Using a Multi-Channel Surface Plasmon Resonance Device

“…Surface plasmon resonance (SPR) biosensors have been used as the dominant method over the years due to their shorter time, reliable and reproducible results, as well as their sensitivity, label-free and real-time detection, and quantitative results [16]. SPR is a phenomenon that measures the formation of surface plasmon polaritons (SPPs) that occur between the interface of a metal and a dielectric substance, typically either a liquid or air.…”

The role of small-angle scattering in structure-based screening applications

“…Another common biophysical approach to fragmentbased screening is SPR spectroscopy (Chavanieu and Pugnière, 2016;Navratilova and Hopkins, 2010;Zeng et al, 2017), where structural and mass changes of biomolecules tethered closely to a metallic substrate alters the local refractive index and thus the total internal reflection behaviour of light beams on the opposing side. Light microscopy for detection brings advantages of millisecond time resolution and thus an ability to directly observe binding kinetics (Daghestani and Day, 2010;Salamon et al, 1997b).…”