Antimicrobial Susceptibility Test for the Determination of Resistant and Susceptible S. aureus and Enterococcus spp. Using a Multi-Channel Surface Plasmon Resonance Device

Abstract

The objective of this study was to investigate the development of a surface plasmon resonance (SPR) sensor platform equipped with multiple channels for the simultaneous determination of methicillin-resistant S. aureus (MRSA), methicillin-susceptible S. aureus (MSSA) and vancomycin-resistant Enterococcus (VRE), and vancomycin-susceptible Enterococcus (VSE). Drug resistance of S. aureus strains against cefoxitin and Enterococcus strains against vancomycin were investigated both using the minimum inhibitory concentration method (MIC) assay and the SPR system equipped with single and multiple channels. The MIC values of MRSA and MSSA ranged from 32 µg/mL to >128 µg/mL and from 1 µg/mL to 4 µg/mL, respectively. The MIC values of VRE and VSE were between 64 to >128 µg/mL and 2–4 µg/mL, respectively. With the multiple-channel system, the angle shifts of MRSA, MSSA, VRE and VSE were found to be −0.030° and −0.260°, −0.010° and −0.090° respectively. The antibiotic-resistant and susceptible strains were distinguished within 3 h for S. aureus strains and within 6 h for Enterococcus strains.

A Molecular Insight into the Synergistic Mechanism of Nigella sativa (Black Cumin) with β-Lactam Antibiotics against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

“…A SEM micrograph analysis showed bacterial cell aggregation without the usual well-defined clustering of S. aureus. This finding is consistent with previous studies that reported the effect of antibiotics on bacterial cells [48,49]. NS alone disrupted the bacterial cell surface and therefore caused destruction, which was evident in the bacterial growth inhibition.…”

Detection of methicillin-resistant Staphylococcus aureus and multidrug resistance isolated from cats in animal clinic at Sidoarjo District, East Java, Indonesia

“…Antibiotic susceptibility tests, such as the disk diffusion test, can be used to identify MRSA; the majority of these tests employed cefoxitin because methicillin antibiotic disk is no longer readily available commercially (Anand et al 2009). Antibiotic susceptibility testing has certain disadvantages compared to identifying mecA or PBP2a (Ozkaya et al 2019). S. aureus isolates that are resistant to cefoxitin continued to undergo ORSAB as a phenotypic test; however, depending on conditions like temperature, it may also vary throughout growth (Maharjan et al 2022).…”

Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

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