Recent advances in self-interference incoherent digital holography

Rosen, Joseph; Anand, Vijayakumar; Kumar, Manoj; Ratnam, Mani Maran; Kelner, Roy; Kashter, Yuval; Bulbul, Angika; Mukherjee, Saswata

doi:10.1364/aop.11.000001

Cited by 166 publications

(104 citation statements)

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“…In COACH, the self-interference is formed between object waves modulated by a quasi-random phase function and a constant phase. As a result, the imaging characteristics of FINCH and COACH are different from one another [17]. Since COACH does not have an image plane as FINCH does, the conventional Fresnel backpropagation method cannot be used for COACH.…”

Section: Introductionmentioning

confidence: 99%

“…In summary, the similarity in the above three methods of COACH, I-COACH, and FINCH is that they are all linear systems regarding intensity such that the same reconstruction mechanism via cross-correlation can be applied to all of them. The differences in the above methods exist in the beam properties, which create the self-interference distribution and the resulting different imaging characteristics [17].…”

Section: Introductionmentioning

confidence: 99%

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Randomly Multiplexed Diffractive Lens and Axicon for Spatial and Spectral Imaging

2020

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A new hybrid diffractive optical element (HDOE) was designed by randomly multiplexing an axicon and a Fresnel zone lens. The HDOE generates two mutually coherent waves, namely a conical wave and a spherical wave, for every on-axis point object in the object space. The resulting self-interference intensity distribution is recorded as the point spread function. A library of point spread functions are recorded in terms of the different locations and wavelengths of the on-axis point objects in the object space. A complicated object illuminated by a spatially incoherent multi-wavelength source generated an intensity pattern that was the sum of the shifted and scaled point spread intensity distributions corresponding to every spatially incoherent point and wavelength in the complicated object. The four-dimensional image of the object was reconstructed using computer processing of the object intensity distribution and the point spread function library.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Randomly Multiplexed Diffractive Lens and Axicon for Spatial and Spectral Imaging

2020

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“…Digital holography can be adopted to fluorescence microscopy for recording and retrieving the 3-D information of incoherent fluorescent objects. [20][21][22][23][24] Our group reported a fluorescent digital holographic system by using a dual-focusing lens with a diffraction grating. 23 In this configuration, the 0-th order unmodulated light is cut out and the interference occurs between the two first orders.…”

Section: Introductionmentioning

confidence: 99%

Common-path multimodal three-dimensional fluorescence and phase imaging system

et al. 2020

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A stable multimodal system is developed by combining two common-path digital holographic microscopes (DHMs): coherent and incoherent, for simultaneous recording and retrieval of three-dimensional (3-D) phase and 3-D fluorescence imaging (FI), respectively, of a biological specimen. The 3-D FI is realized by a single-shot common-path off-axis fluorescent DHM developed recently by our group. In addition, we accomplish, the phase imaging by another single-shot, highly stable common-path off-axis DHM based on a beam splitter. In this DHM configuration, a beam splitter is used to divide the incoming object beam into two beams. One beam serves as the object beam carrying the useful information of the object under study, whereas another beam is spatially filtered at its Fourier plane by using a pinhole and it serves as a reference beam. This DHM setup, owing to a common-path geometry, is less vibration-sensitive and compact, having a similar field of view but with high temporal phase stability in comparison to a two-beam Mach-Zehnder-type DHM. The performance of the proposed common-path DHM and the multimodal system is verified by conducting various experiments on fluorescent microspheres and fluorescent protein-labeled living cells of the moss Physcomitrella patens. Moreover, the potential capability of the proposed multimodal system for 3-D live fluorescence and phase imaging of the fluorescent beads is also demonstrated. The obtained experimental results corroborate the feasibility of the proposed multimodal system and indicate its potential applications for the analysis of functional and structural behaviors of a biological specimen and enhancement of the understanding of physiological mechanisms and various biological diseases. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…However, these efforts make the system more complicated and the whole process becomes time-consuming 32,33 . Recently, holographic fluorescence microscopy has been possible due to the advent of Fresnel incoherent correlation holography (FINCH) [34][35][36][37] , as fluorescence light is naturally incoherent 38 . FINCH is based on the self-interference principle stated that two or more beams originating from the same source point are mutually coherent and interfere to form the in-line digital hologram 37 .…”

mentioning

confidence: 99%

“…Recently, holographic fluorescence microscopy has been possible due to the advent of Fresnel incoherent correlation holography (FINCH) [34][35][36][37] , as fluorescence light is naturally incoherent 38 . FINCH is based on the self-interference principle stated that two or more beams originating from the same source point are mutually coherent and interfere to form the in-line digital hologram 37 . In FINCH, the incident wave originated from each object point is allowed to split into two waves with different curvatures by using an appropriate optical component (say an appropriate pattern, e.g., a grating pattern, displayed on a spatial light modulator) and an interference pattern is produced by the recombination of these waves at an appropriate overlapped region in the image sensor.…”

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confidence: 99%

Digital Holographic Multimodal Cross-Sectional Fluorescence and Quantitative Phase Imaging System

Kumar

Quan

Awatsuji

et al. 2020

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We present a multimodal imaging system based on simple off-axis digital holography, for simultaneous recording and retrieval of cross-sectional fluorescence and quantitative phase imaging of the biological specimen. Synergism in the imaging capabilities can be achieved by incorporating two off-axis digital holographic microscopes integrated to record different information at the same time. The cross-sectional fluorescence imaging is realized by a common-path configuration of the single-shot off-axis incoherent digital holographic system. The quantitative phase imaging, on the other hand, is achieved by another off-axis coherent digital holographic microscopy operating in transmission mode. The fundamental characteristics of the proposed multimodal system are confirmed by performing various experiments on fluorescent beads and fluorescent protein-labeled living cells of the moss Physcomitrella patens lying at different axial depth positions. Furthermore, the cross-sectional live fluorescence and phase imaging of the fluorescent beads are demonstrated by the proposed multimodal system. The experimental results presented here corroborate the feasibility of the proposed system and indicate its potential in the applications to analyze the functional and structural behavior of biological cells and tissues. Over the past decade, multimodal systems based on quantitative phase imaging in combination with fluorescence imaging have been developed considerably due to its several advantages 1-12. The phase imaging reveals the structural information by exploiting the optical path-length shifts through the biological specimen, while fluorescence imaging provides functional details of the specific molecules of interest in the biological specimen. Therefore, a hybrid multimodal imaging system comprising both these systems offers to precisely visualize and delineate structural and functional information in the biological specimen on a single platform at the same time. The quantitative phase imaging technique 13-16 is an emerging powerful technology of a new paradigm in general imaging and biomedical applications that provides quantitative information including the structure and dynamics of the transparent specimens, which is not easily obtained with the conventional optical imaging techniques. The technique employs the principle of optical interferometry to measure the optical field (i.e. amplitude and phase information). In recent years, quantitative phase imaging has received substantial interest and opened the door for quantifying dynamic structural information of biological cells with nanoscale sensitivity. Popescu et al. 17 reported the use of phase imaging for monitoring cell growth, characterizing cellular motility, and investigating the subcellular motions of living cells. The technique has also found applications for various investigations of cells and tissues including measurement of 3D profiling and tracking 15 , refractive index 18 , spectral dispersion 19 , optical path length 20 , dry mass localization 21 , and optim...

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Recent advances in self-interference incoherent digital holography

Cited by 166 publications

References 118 publications

Randomly Multiplexed Diffractive Lens and Axicon for Spatial and Spectral Imaging

Randomly Multiplexed Diffractive Lens and Axicon for Spatial and Spectral Imaging

Common-path multimodal three-dimensional fluorescence and phase imaging system

Digital Holographic Multimodal Cross-Sectional Fluorescence and Quantitative Phase Imaging System

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