Recent Advances in Scaffolding from Natural-Based Polymers for Volumetric Muscle Injury

Nuge, Tamrin; Liu, Ziqian; Liu, Xiaoling; Ang, Bee Chin; Andriyana, Andri; Cornelis, Hendrik Simon; Hoque, Md Enamul

doi:10.2139/ssrn.3604497

Cited by 2 publications

(3 citation statements)

References 26 publications

(27 reference statements)

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“…4 In the last three decades, several three-dimensional (3D) SMT culture systems have been developed to mimic the native muscle microenvironment and understand skeletal muscle function, plasticity, and disease. 1,5 This understanding not only improved cell culture models for biomedical research 6,7 and engineered tissue grafts for medical implantation, 2,8,9 but also enabled controllable biomachines with dynamic abilities ( i . e ., biohybrid robots).…”

Section: Introductionmentioning

confidence: 99%

“…The copyright holder for this preprint this version posted January 15, 2023. ; https://doi.org/10.1101/2023.01.12.523732 doi: bioRxiv preprint microenvironment and understand skeletal muscle function, plasticity, and disease. 1,5 This understanding not only improved cell culture models for biomedical research 6,7 and engineered tissue grafts for medical implantation, 2,8,9 but also enabled controllable biomachines with dynamic abilities (i.e., biohybrid robots). [10][11][12] Through the years, SMT was engineered in vitro via approaches that aim at replicating the ultrastructure of native muscle tissue, which is composed of highly co-oriented myofibers.…”

Section: Introductionmentioning

confidence: 99%

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Perfusable biohybrid designs for bioprinted skeletal muscle tissue

Filippi

Yaşa

Giachino

et al. 2023

Preprint

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Engineered, centimeter-scale skeletal muscle tissue (SMT) can mimic muscle pathophysiology to study development, disease, regeneration, drug response, and motion. Macroscale SMT requires perfusable channels to guarantee cell survival and support elements to enable mechanical cell stimulation and uniaxial myofiber formation. Here, stable biohybrid designs of centimeter-scale SMT are realized via extrusion-based bioprinting of an optimized polymeric blend based on gelatin methacryloyl and sodium alginate, which can be accurately co-printed with other inks. A perfusable microchannel network is designed to functionally integrate with perfusable anchors for insertion into a maturation culture template. The results demonstrate that (i) co-printed synthetic structures display highly coherent interfaces with the living tissue; (ii) perfusable designs preserve cells from hypoxia all over the scaffold volume; and (iii) constructs can undergo passive mechanical tension during matrix remodeling. Extrusion-based multimaterial bioprinting with our inks and design realizes in vitro matured biohybrid SMT for biomedical, nutritional, and robotic applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Perfusable biohybrid designs for bioprinted skeletal muscle tissue

Filippi

Yaşa

Giachino

et al. 2023

Preprint

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“…The cells exhibit clonogenic capability with 36 h doubling time and can be cultured up to 300 passages and capable of differentiating into all three primary germ layers (ecto‐, meso‐, and endoderm) 3 . AFSCs have achieved promising therapeutic results in the context of neoorgan and tissue regeneration, including heart, muscle, and kidney thanks to paracrine factors secreted by the AFSCs 4 . The paracrine factors such as growth factors and cytokines evoked critical reactions including cell proliferation, cytoprotection, and migration 5 .…”

Section: Introductionmentioning

confidence: 99%

Accelerated wound closure: Systematic evaluation of cellulose acetate effects on biologically active molecules release from amniotic fluid stem cells

Nuge

Liu

Tshai

et al. 2021

Biotech and App Biochem

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Despite a lot of intensive research on cell-scaffold interaction, the focus is mainly on the capacity of construct scaffolds to regulate cell mobility, migration, and cytotoxicity. The effect of the scaffold's topographical and material properties on the expression of biologically active compounds from stem cells is not well understood. In this study, the influence of cellulose acetate (CA) on the electrospinnability of gelatin and the roles of gelatin-cellulose acetate (Ge-CA) on modulating the release of biologically active compounds from amniotic fluid stem cells (AFSCs) is emphasized. It was found that the presence of a small amount of CA could provide a better microenvironment that mimics AFSCs' niche. However, a large amount of CA exhibited no significant effect on AFSCs migration and infiltration. Further study on the effect of surface topography and mechanical properties on AFSCs showed that the tailored microenvironment provided by the Ge-CA scaffolds had transduced physical cues to biomolecules released into the culture media. It was found that the AFSCs seeded on electrospun scaffolds with less CA proportions have profound effects on the secretion of metabolic compounds compared to those with higher CA contained and gelatin coating.The enhanced secretion of biologically active molecules by the AFSCs on the electrospun scaffolds was proven by the accelerated wound closure on the injured human dermal fibroblast (HDF) model. The rapid HDF cell migration could be anticipated due to a higher level of paracrine factors in AFSCs media. Our study demonstrates that the fibrous topography and mechanical properties of the scaffold are a key material property that modulates the high expression of

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Recent Advances in Scaffolding from Natural-Based Polymers for Volumetric Muscle Injury

Abstract: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Cited by 2 publications

References 26 publications

Perfusable biohybrid designs for bioprinted skeletal muscle tissue

Perfusable biohybrid designs for bioprinted skeletal muscle tissue

Accelerated wound closure: Systematic evaluation of cellulose acetate effects on biologically active molecules release from amniotic fluid stem cells

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