Accelerated wound closure: Systematic evaluation of cellulose acetate effects on biologically active molecules release from amniotic fluid stem cells

Abstract

Despite a lot of intensive research on cell-scaffold interaction, the focus is mainly on the capacity of construct scaffolds to regulate cell mobility, migration, and cytotoxicity. The effect of the scaffold's topographical and material properties on the expression of biologically active compounds from stem cells is not well understood. In this study, the influence of cellulose acetate (CA) on the electrospinnability of gelatin and the roles of gelatin-cellulose acetate (Ge-CA) on modulating the release of biologically active compounds from amniotic fluid stem cells (AFSCs) is emphasized. It was found that the presence of a small amount of CA could provide a better microenvironment that mimics AFSCs' niche. However, a large amount of CA exhibited no significant effect on AFSCs migration and infiltration. Further study on the effect of surface topography and mechanical properties on AFSCs showed that the tailored microenvironment provided by the Ge-CA scaffolds had transduced physical cues to biomolecules released into the culture media. It was found that the AFSCs seeded on electrospun scaffolds with less CA proportions have profound effects on the secretion of metabolic compounds compared to those with higher CA contained and gelatin coating.The enhanced secretion of biologically active molecules by the AFSCs on the electrospun scaffolds was proven by the accelerated wound closure on the injured human dermal fibroblast (HDF) model. The rapid HDF cell migration could be anticipated due to a higher level of paracrine factors in AFSCs media. Our study demonstrates that the fibrous topography and mechanical properties of the scaffold are a key material property that modulates the high expression of

The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

“…Harnessing these effects could be used to improve outcomes for wound healing . Similarly, closure rates were enhanced when human dermal fibroblasts were treated with a conditioned medium from amniotic fluid stem cells cultured on randomly oriented (20 nm) electrospun fibers compared to flat surfaces . Outside of wound healing, increased secretion of proteins including bFGF, TGF-β1, and interleukin-8 (IL-8) by hMSCs on electrospun PLCL increased their chondrogenic effects, with clear differences in the secretome of cells on aligned versus random fibers and confirmation that these effects were mediated by mechanotransductive processes, including FAK-ERK signaling …”

“…288 Similarly, closure rates were enhanced when human dermal fibroblasts were treated with a conditioned medium from amniotic fluid stem cells cultured on randomly oriented (20 nm) electrospun fibers compared to flat surfaces. 289 Outside of wound healing, increased secretion of proteins including bFGF, TGF-β1, and interleukin-8 (IL-8) by hMSCs on electrospun PLCL increased their chondrogenic effects, with clear differences in the secretome of cells on aligned versus random fibers and confirmation that these effects were mediated by mechanotransductive processes, including FAK-ERK signaling. 290 Additional effects of surface topography have also been found in systems such as thermally induced phase separation (TIPS)based microcarriers, whereby the micropore structure altered the secretion of angiogenic factors by hMSCs 291 roughened PEI surfaces, which induced changes in the MSC secretome that altered effects on the senescence of neighboring cells.…”

Synthesis, Properties, Applications, and Future Prospective of Cellulose Nanocrystals

“…Nanocellulose membranes might act as an infection barrier, stop fluid loss, relieve pain, make medications easier to apply, and absorb the inflammatory fluids throughout the disease process [107]. They are suited for wound dressings because of characteristics including flexible surface chemistry, anisotropy, optical transparency, high superficial area, high elastic modulus, low thermal expansion coefficient, high water absorption capacity, and biocompatibility.…”