Recent Advances in Quinone Methide Chemistry for Protein-Proximity Capturing

Abstract

Here we summarize the most recent findings in the chemical-, photo-, or enzyme-triggered generation of nitrogen and oxygen anions leading to the formation of quinone methide intermediates (QMIs). This short review is divided into two categories: generation of nitrogen and oxygen anions. Based on quinone methide intermediates (QMIs), proximate capture of a wide range of proteins has been widely determined and studied. Generally, the triggers include, photoirradiation using 365/254 nm UV light, small molecules (ROS/TBAF/s-tetrazine), metal catalysis (iridium catalysis), and enzymes (NQO1/β-galactosidase). New directions including far-red light, heat, force, microwave, and more practical approaches are explored and illustrated.1 Introduction2 Generation of the Nitrogen Anion3 Generation of the Oxygen Anion4 Conclusion

Sequencing, Physiological Regulation, and Representative Disease Research Progress of RNA m6A Modification

“…The impact of these modifications on viral characteristics remains elusive, as well as the types, molecular mechanisms, and targets of these modifications. 136 At present, the role played by these new chemical modifications (NAD, prenyl, and oQ modifications) remains unknown, [137][138][139][140][141][142][143][144][145] although it has been speculated that they may help the virus avoid host attacks (for example m 6 A, m 1 A, and Ψ). Therefore, elucidating the types and characteristics of these chemical modifications is expected to provide new clues for the fight against COVID-19 and many other RNA-relevant viruses.…”