Recent advances in point-of-care testing of COVID-19

Lee, Sungwoon; Bi, Liyan; Chen, Hao; Lin, Dong; Mei, Rongchao; Wu, Yixuan; Chen, Lingxin; Joo, Sang-Woo; Choo, Jaebum

doi:10.1039/d3cs00709j

Cited by 17 publications

(8 citation statements)

References 202 publications

(218 reference statements)

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“…Immunoassays (IAs) have become widely adopted in the field of diagnostics for diseases and have emerged as one of the largest and fastest growing segments of in vitro diagnostics. However, conventional IAs, with their time-consuming and labor-intensive protocols for immunobinding, limit their efficacy in point-of-care testing (POCT) for on-site detection. − …”

Section: Introductionmentioning

confidence: 99%

Centrifugo-Pneumatic Reciprocating Flowing Coupled with a Spatial Confinement Strategy for an Ultrafast Multiplexed Immunoassay

Qian,

Li,

Wang

et al. 2024

Anal. Chem.

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Immunoassays serve as powerful diagnostic tools for early disease screening, process monitoring, and precision treatment. However, the current methods are limited by high costs, prolonged processing times (>2 h), and operational complexities that hinder their widespread application in point-of-care testing. Here, we propose a novel centrifugo-pneumatic reciprocating flowing coupled with spatial confinement strategy, termed PRCM, for ultrafast multiplexed immunoassay of pathogens on a centrifugal microfluidic platform. Each chip consists of four replicated units; each unit allows simultaneous detection of three targets, thereby facilitating high-throughput parallel analysis of multiple targets. The PRCM platform enables sequential execution of critical steps such as solution mixing, reaction, and drainage by coordinating inherent parameters, including motor rotation speed, rotation direction, and acceleration/deceleration. By integrating centrifugal-mediated pneumatic reciprocating flow with spatial confinement strategies, we significantly reduce the duration of immune binding from 30 to 5 min, enabling completion of the entire testing process within 20 min. As proof of concept, we conducted a simultaneous comparative test on-and off-the-microfluidics using 12 negative and positive clinical samples. The outcomes yielded 100% accuracy in detecting the presence or absence of the SARS-CoV-2 virus, thus highlighting the potential of our PRCM system for multiplexed point-of-care immunoassays.

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Section: Introductionmentioning

confidence: 99%

Centrifugo-Pneumatic Reciprocating Flowing Coupled with a Spatial Confinement Strategy for an Ultrafast Multiplexed Immunoassay

Qian,

Li,

Wang

et al. 2024

Anal. Chem.

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“…24,25 Numerous studies have exploited PADs to develop point-of-care and POU testing platforms. 26 Such testing systems have been performed using immunosorbents, 27 molecularly imprinted sorbents, 28 or hydrophobic paper 29 coupled with colorimetric or spectrouorimetric detection. Nevertheless, we performed a literature survey that revealed that no PAD is available for the POU determination of the water content in ethanol.…”

Section: Introductionmentioning

confidence: 99%

Simple colorimetric paper-based test strip for point-of-use quality testing of ethanol-based hand sanitizers

El-Hassanein,

Mansour,

Hammad

et al. 2024

RSC Adv.

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“…Since the introduction of the micro-total analysis system (micro-TAS) by Manz in the early 1990s [ 1 ], microfluidic systems have been applied across diverse domains, including biomedical diagnosis, chemical synthesis and analysis, drug discovery, and human healthcare [ 2 , 3 ]. The global impact of the coronavirus disease of 2019 (COVID-19) has underscored the urgent need for on-site diagnostic systems capable of swiftly and accurately analyzing minute clinical samples [ 4 ]. Microfluidic devices are viewed as a pivotal technology for meeting this demand.…”

Section: Introductionmentioning

confidence: 99%

Microfluidics for disease diagnostics based on surface-enhanced raman scattering detection

Yu,

Park,

Lee

et al. 2024

Nano Convergence

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This review reports diverse microfluidic systems utilizing surface-enhanced Raman scattering (SERS) detection for disease diagnosis. Integrating SERS detection technology, providing high-sensitivity detection, and microfluidic technology for manipulating small liquid samples in microdevices has expanded the analytical capabilities previously confined to larger settings. This study explores the principles and uses of various SERS-based microfluidic devices developed over the last two decades. Specifically, we investigate the operational principles of documented SERS-based microfluidic devices, including continuous-flow channels, microarray-embedded microfluidic channels, droplet microfluidic channels, digital droplet channels, and gradient microfluidic channels. We also examine their applications in biomedical diagnostics. In conclusion, we summarize the areas requiring further development to translate these SERS-based microfluidic technologies into practical applications in clinical diagnostics.

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Recent advances in point-of-care testing of COVID-19

Abstract: This review explores various point-of-care optical diagnostic systems combined with microdevices developed during the recent COVID-19 pandemic for clinical diagnostics.

Cited by 17 publications

References 202 publications

Centrifugo-Pneumatic Reciprocating Flowing Coupled with a Spatial Confinement Strategy for an Ultrafast Multiplexed Immunoassay

Centrifugo-Pneumatic Reciprocating Flowing Coupled with a Spatial Confinement Strategy for an Ultrafast Multiplexed Immunoassay

Simple colorimetric paper-based test strip for point-of-use quality testing of ethanol-based hand sanitizers

Microfluidics for disease diagnostics based on surface-enhanced raman scattering detection

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