Recent Advances in Plant Transformation

Barampuram, Shyamkumar; Zhang, Zhanyuan J.

doi:10.1007/978-1-61737-957-4_1

Cited by 108 publications

(75 citation statements)

References 182 publications

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“…The genetic manipulation of sugarcane requires the availability of an efficient transformation system that directly depends on the embryogenic capability of the callus and its subsequent regeneration (Barampuram and Zhang, 2011). In the present study, 4 elite sugarcane varieties from Pakistan with good yield potential were selected to become herbicide (glyphosate)-tolerant.…”

Section: Introductionmentioning

confidence: 99%

Herbicide-tolerant sugarcane (Saccharum officinarum L.) plants: an unconventional method of weed removal

Nasir¹,

Tabassum²,

Qamar³

et al. 2014

Turk J Biol

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Unnecessary weed growth in sugarcane fields forces plants to compete for nutrients and sunlight for survival, which most often leads to significant yield losses. As chemical herbicides cannot differentiate between crop plants and weeds, the development of herbicide-tolerant crops is anticipated. Four sugarcane varieties, CPF-234, CPF-213, HSF-240, and CPF-246, were used to develop glyphosate herbicide tolerance. A glyphosate-tolerant gene of 1368 bp cloned directionally under the 35S promoter with the β-glucuronidase (GUS) reporter gene was used as the transgene. Through the biolistic transformation of sugarcane, calli of all cultivars were transformed with glyphosate-tolerant gene constructs. Efficient regeneration conditions were optimized on 1.5-2.5 mg/L kinetin, 1.5-2.5 mg/L 6-benzylaminopurine (BAP), and 1 mg/L gibberellic acid (GA 3 ). The transformants exhibited the best shoot regeneration on a medium containing 2 mg/L kinetin, 2 mg/L BAP, 2 mg/L GA 3 , and 1 mg/L indole-3-acetic acid. Based on initial screenings through GUS assay, the transformation efficiency was 22%, 32%, 17%, and 13% for cultivars 246, 234, 213, and 240, respectively. In transformed sugarcane plants, the transgene of 1368 bp was amplified. ELISA with gene-specific coated monoclonal IgG confirmed the transgene protein expression. It was revealed that all acclimatized transgenic sugarcane plants survived the glyphosate spray application of 900 mL/0.404 ha, except for the control nontransformed plants. However, at spray application of 1100 mL/0.404 ha, transgenic plants having the transgene protein OD of 0.2 to 1.0 did not survive, while those that had a transgene protein OD range of between 1.0 and 2.0 did. In addition, weeds growing alongside transgenic sugarcane plants turned brown and subsequently died at glyphosate spray applications of both 900 and 1100 mL/0.404 ha.

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Section: Introductionmentioning

confidence: 99%

Herbicide-tolerant sugarcane (Saccharum officinarum L.) plants: an unconventional method of weed removal

Nasir¹,

Tabassum²,

Qamar³

et al. 2014

Turk J Biol

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“…In addition, the main physical barrier to electroporation maybe is the thickness of recipient cell walls [8].…”

Section: Electroporation-mediated Transformationmentioning

confidence: 99%

Novel Techniques for Gene Delivery into Plants and Its Applications for Disease Resistance in Crops

Rashid¹,

Lateef²

2016

AJPS

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From the early past to the present, biotechnologies have produced the ability to genetically transform a wide variety of plant species. The plant transformation technologies have changed the face of agriculture and plant biology. Plant genetic transformation is one of the key technologies for crop improvement in addition to emerging approach for producing recombinant proteins in plants. Both plastid genomes and plant nuclear can be genetically modified. Until now, essential functional differences between the prokaryotic-like genome of the plastid and the eukaryotic genome of the plant cell nucleus will have an impact on characteristics of transgenic organism. Thus, the main goals are to generate transgenic plants with the traits of interest as well as minimizing the amount of transgenic DNA in plants while maximizing stability of gene expression and trait performance. In this review, two broad groups of gene delivery methods will be discussed namely, (bilogical and physical methods) and subsequently there applications for improving disease resistance will be discussed.

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“…10 Die entstehende Neukombination der Erbinformation wäre theoretisch also auch auf natürlichem Wege erreichbar -anders als bei transgenen Pflanzen, bei denen bei der Übertragung von Genen (zum Teil weite) Artgrenzen überschritten werden. Für die Verwendung von neuen Genkombinationen, welche in vitro durch die Neuanordnung arteigener funktioneller Elemente (wie etwa Promotoren, kodierende Regionen oder terminale Re-9 Siehe hierzu ausführlich Müller-Röber et al, 2009:242 f. Übersichtsartikel zu aktuellen Fortschritten der Pflanzentransformation bieten Barampuram/Zhang, 2011;Rafsanjani et al,2012und Rivera et al, 2012 10 Auf die grundlegenden biologischen Prinzipien der Cisgenetik und ihre potentiellen Vorteile gegenüber der Herstellung transgener Pflanzen wurde bereits im "Zweiten Gentechnologiebericht" ) sowie in den erschienenen Themenbänden zur grünen Gentechnologie ausführlich eingegangen. gionen) 11 als Konstrukt entstanden sind, wurde der Begriff der Intragenese etabliert ( Holme et al, 2013;siehe Abbildung 1).…”

Section: Cisgene Und Intragene Pflanzenunclassified

7. Themenbereich grüne Gentechnologie: Pflanzenzüchtung und Agrarwirtschaft

Müller‐Röber¹,

Marx-Stölting²

2015

Dritter Gentechnologiebericht

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Recent Advances in Plant Transformation

Cited by 108 publications

References 182 publications

Herbicide-tolerant sugarcane (Saccharum officinarum L.) plants: an unconventional method of weed removal

Herbicide-tolerant sugarcane (Saccharum officinarum L.) plants: an unconventional method of weed removal

Novel Techniques for Gene Delivery into Plants and Its Applications for Disease Resistance in Crops

7. Themenbereich grüne Gentechnologie: Pflanzenzüchtung und Agrarwirtschaft

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