Recent advances in nanomaterial-based biosensors for the detection of exosomes

Zhang, Linan; Gu, Chunchuan; Wen, Jiajun; Liu, Guangxian; Liu, Hongying; Li, Lihua

doi:10.1007/s00216-020-03000-0

Cited by 49 publications

(53 citation statements)

References 122 publications

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“…Last but not least, although the focus of this article is on protein quantification, other omics studies such as genomics, metabolomics, cellomics, and exomics have also shown important clinical implications [ 82 – 87 ]. Indeed, many of the strategies on sensitive protein detection could be adapted for quantifying other biomolecules, vesicles, or cells.…”

Section: Perspectives and Conclusionmentioning

confidence: 99%

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

Momenbeitollahi

Cloet

2021

Anal Bioanal Chem

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Proteins are one of the main constituents of living cells. Studying the quantities of proteins under physiological and pathological conditions can give valuable insights into health status, since proteins are the functional molecules of life. To be able to detect and quantify low-abundance proteins in biofluids for applications such as early disease diagnostics, sensitive analytical techniques are desired. An example of this application is using proteins as biomarkers for detecting cancer or neurological diseases, which can provide early, lifesaving diagnoses. However, conventional methods for protein detection such as ELISA, mass spectrometry, and western blotting cannot offer enough sensitivity for certain applications. Recent advances in optical-based micro- and nano-biosensors have demonstrated promising results to detect proteins at low quantities down to the single-molecule level, shining lights on their capacities for ultrasensitive disease diagnosis and rare protein detection. However, to date, there is a lack of review articles synthesizing and comparing various optical micro- and nano-sensing methods of enhancing the limits of detections of the antibody-based protein assays. The purpose of this article is to critically review different strategies of improving assay sensitivity using miniaturized biosensors, such as assay miniaturization, improving antibody binding capacity, sample purification, and signal amplification. The pros and cons of different methods are compared, and the future perspectives of this research field are discussed.

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Section: Perspectives and Conclusionmentioning

confidence: 99%

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

Momenbeitollahi

Cloet

2021

Anal Bioanal Chem

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“…It must be noted that in the EV context, compared to conventional biomarkers, the importance of pre-analytical variables is increased because of the difficulty of many techniques to work in their optimal sensitivity range, with many traditional instruments remaining limited in their capacity to discriminate EVs from instrument noise, and thus making artifacts exert major impact on all subsequent information. For this reasons, many position papers, guidelines, and road maps from the ISEV community [ 22 , 33 , 34 , 35 ] have been published, as have emerging techniques for the easy translation of EV-based biomarkers to the clinical setting [ 25 , 36 , 37 ].…”

Section: Extracellular Vesicles In Strokementioning

confidence: 99%

Extracellular Vesicles in Regeneration and Rehabilitation Recovery after Stroke

Gualerzi

Picciolini

Rodà

et al. 2021

Biology

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Patients that survive after a stroke event may present disabilities that can persist for a long time or permanently after it. If stroke prevention fails, the prompt and combinatorial intervention with pharmacological and rehabilitation therapy is pivotal for the optimal recovery of patients and the reduction of disabilities. In the present review, we summarize some key features of the complex events that occur in the brain during and after the stroke event, with a special focus on extracellular vesicles (EVs) and their role as both carriers of biomarkers and potential therapeutics. EVs have already demonstrated their ability to be used for diagnostic purposes for multiple brain disorders and could represent valuable tools to track the regenerative and inflammatory processes occurring in the injured brain after stroke. Last, but not least, the use of artificial or stem cell-derived EVs were proved to be effective in stimulating brain remodeling and ameliorating recovery after stroke. Still, effective biomarkers of recovery are needed to design robust trials for the validation of innovative therapeutic strategies, such as regenerative rehabilitation approaches.

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“…It could also comprehensively reflect the state information of tumor cell state [2,4] and pave the way for precision treatment and personalized medicine [5]. Thus far, several novel biomarkers specific to various cancers in fluid circulation have been found and played important roles in cancer diagnosis, especially exosome [6,7], of which superiorities lie in comprehensive information, high content in biofluids (1 Â 10 9 particles/mL in the blood), abundant cargos, stable circulation, easy capture, and enrichment of surface proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Biosensors are nowadays ubiquitous in many areas, especially in biomedical research, disease diagnosis [30], food safety [31], and drug discovery [32]. As a promising candidate for conquering the challenges at this stage, biosensors could boost the field of liquid biopsy [33,34] owing to their small size, fast response, and high sensitivity [6,35]. In the past years, researchers have proposed a large number of biosensors for exosome assays with extraordinary performance and then applied them in medical and biological fields [34,36].…”

Section: Introductionmentioning

confidence: 99%

“…Several reviews that refer to exosome detection have been recently published [28,35,38]. The present article focused on the superiorities of exosomes acting as a biomarker in cancer diagnosis, and various types of biosensors, including electrochemical biosensor, colorimetric biosensors, fluorescence-based biosensors, surface plasmon resonance (SPR)-based biosensors, surfaceenhanced Raman scattering (SERS)-based biosensors, and microfluidic-based biosensors, were comprehensively summarized, with an emphasis on their analytical performances and further challenges in the clinic diagnosis of cancer and the potential solutions.…”

Section: Introductionmentioning

confidence: 99%

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Biosensor-based assay of exosome biomarker for early diagnosis of cancer

et al. 2021

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Cancer imposes a severe threat to people’s health and lives, thus pressing a huge medical and economic burden on individuals and communities. Therefore, early diagnosis of cancer is indispensable in the timely prevention and effective treatment for patients. Exosome has recently become an attractive cancer biomarker in noninvasive early diagnosis because of the unique physiology and pathology functions, which reflects remarkable information regarding the cancer microenvironment, and plays an important role in the occurrence and evolution of cancer. Meanwhile, biosensors have gained great attention for the detection of exosomes due to their superior properties, such as convenient operation, real-time readout, high sensitivity, and remarkable specificity, suggesting promising biomedical applications in the early diagnosis of cancer. In this review, the latest advances of biosensors regarding the assay of exosomes were summarized, and the superiorities of exosomes as markers for the early diagnosis of cancer were evaluated. Moreover, the recent challenges and further opportunities of developing effective biosensors for the early diagnosis of cancer were discussed.

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Recent advances in nanomaterial-based biosensors for the detection of exosomes

Cited by 49 publications

References 122 publications

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

Extracellular Vesicles in Regeneration and Rehabilitation Recovery after Stroke

Biosensor-based assay of exosome biomarker for early diagnosis of cancer

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