Biosensor-based assay of exosome biomarker for early diagnosis of cancer

Deng, Ying; Sun, Zhaowei; Wang, Lei; Wang, Minghui; Yang, Jie; Li, Genxi

doi:10.1007/s11684-021-0884-z

Cited by 16 publications

(8 citation statements)

References 148 publications

(161 reference statements)

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“…Transmission electron microscopy showed that the exosomes had circular structures (Figure 2b). Because certain proteins highly enriched in exosomes, such as HSP70, CD9, CD82, Tsg101, and CD63, are used as markers to identify exosomes (Deng et al, 2022), including SC exosomes (Wei et al, 2019), we validated their expression in the isolated particles (Figure 2c). Our results are consistent with exosome characteristics (Wei et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…Finally, exosomes were purified using a qEVoriginal/35 nm Gen 2 column (ICO‐35, Izon Science, Bellaire, TX, USA). Fractions of 2 mL were eluted with PBS, and the protein composition was analyzed by immunoblotting using antibodies targeting exosome markers HSP70A8, CD9, and CD81 (Deng et al, 2022; Mathieu et al, 2021). Exosome‐positive fractions were collected for subsequent experiments.…”

Section: Methodsmentioning

confidence: 99%

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Schwann cell‐derived exosomes promote lung cancer progression via miRNA‐21‐5p

Zhou,

Zhang,

et al. 2024

Glia

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Schwann cells (SCs), the primary glial cells of the peripheral nervous system, which have been identified in many solid tumors, play an important role in cancer development and progression by shaping the tumor immunoenvironment and supporting the development of metastases. Using different cellular, molecular, and genetic approaches with integrated bioinformatics analysis and functional assays, we revealed the role of human SC‐derived exosomal miRNAs in lung cancer progression in vitro and in vivo. We found that exosomal miRNA‐21 from SCs up‐regulated the proliferation, motility, and invasiveness of human lung cancer cells in vitro, which requires functional Rab small GTPases Rab27A and Rab27B in SCs for exosome release. We also revealed that SC exosomal miRNA‐21‐5p regulated the functional activation of tumor cells by targeting metalloprotease inhibitor RECK in tumor cells. Integrated bioinformatic analyses showed that hsa‐miRNA‐21‐5p is associated with poor prognosis in patients with lung adenocarcinoma and can promote lung cancer progression through multiple signaling pathways including the MAPK, PI3K/Akt, and TNF signaling. Furthermore, in mouse xenograft models, SC exosomes and SC exosomal hsa‐miRNA‐21‐5p augmented human lung cancer cell growth and lymph node metastasis in vivo. Together our data revealed, for the first time, that SC‐secreted exosomes and exosomal miRNA‐21‐5p promoted the proliferation, motility, and spreading of human lung cancer cells in vitro and in vivo. Thus, exosomal miRNA‐21 may play an oncogenic role in SC‐accelerated progression of lung cancer and this pathway may serve as a new therapeutic target for further evaluation.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Schwann cell‐derived exosomes promote lung cancer progression via miRNA‐21‐5p

Zhou,

Zhang,

et al. 2024

Glia

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“…Exosomes are cell-secreted, nanosized bilayer membrane extracellular vesicles with diameters ranging from 40-100 nm [6]. Exosomes can carry a diferent number of bioactive molecules, including proteins, DNA, various types of RNAs (such as miRNAs, mRNAs, lncRNAs, and circular RNAs), lipids, and metabolites according to the cells of origin via cellular communication [7][8][9]. Exosomes were initially viewed as waste products produced during diferent physiologic cell metabolisms [10].…”

Section: Introductionmentioning

confidence: 99%

“…In turn, various abnormal biological alterations of diferent cells (including tumor cells) can also be refected by detecting the expression of exosomal molecules (including miRNAs) in body fuids [14]. Recent studies have shown that tumor cells can secrete many more exosomes than normal cells, and abnormal exosomes can be easily detected in various body fuids, such as urine, saliva, ascites, or blood [9]. Many studies have shown that the abnormal expression of miRNA in tumor exosomes is closely related to various biological changes, such as angiogenesis, tumor invasion, and metastasis [14,15].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia-Induced miR-210 Promotes Endothelial Cell Permeability and Angiogenesis via Exosomes in Pancreatic Ductal Adenocarcinoma

Ding

Quan

et al. 2022

Biochemistry Research International

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Background. Exosomes have been proven to play important diagnostic, regulatory, or communication roles in tumorigenesis, tumor progression, or metastasis; in recent studies, lots of molecules, including miRNAs, were found to be aberrantly expressed in tumor exosomes and were correlated with tumor development. However, studies about the expression, relationship, or control mechanisms of miRNAs in exosomes in pancreatic ductal adenocarcinoma (PDAC) are scarce and urgently needed. The aim of this article was to identify and investigate abnormally expressed miRNAs in PDAC exosomes in vivo and in vitro. Methods. Microarray studies were used to detect aberrantly expressed miRNAs in PDAC exosomes, and miR-210 expression in cells or exosomes was further analyzed by qRT-PCR. Bioinformatics analyses, dual-luciferase assays, WB, and other assays were utilized to explore the miRNA molecular mechanisms. The living cell coculture model and immunofluorescence analysis were employed to image the communication between PDAC cells and endothelial cells. Other biological experiments in the study include a real-time intravital imaging system, EdU, transwell, xenograft models, and so on. Results. miR-210 is significantly expressed in PDAC exosomes and malignant cells. High miR-210 significantly facilitated tumor angiogenesis, cell invasion, and proliferation in PDAC cells. Further mechanistic detection revealed that miR-210 negatively regulated EFNA3 expression and participated in the PI3K/AKT/VEGFA or Wnt/Β-catenin/RHOA pathways, thus promoting tumor angiogenesis and cellular permeability. PDAC cells promote endothelial angiogenesis or permeability via miR-210 transmission by tumor exosomes. Exosomal miR-210 promotes PDAC progression in vivo. Further detection of PDAC plasma exosomal miR-210 suggests that exosomal miR-210 expression was high and significantly associated with vascular invasion and TNM stage and was an independent risk factor for PDAC overall survival. Conclusions. PDAC cell-secreted exosomes could promote angiogenesis and cellular permeability of neighboring endothelial angiogenesis or permeability via miR-210 transmission. Exosomal miR-210 may play important roles in tumor biology and may be a useful prognostic marker in PDAC.

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“…A biosensor is a device that can produce a measurable signal proportional to the concentration of the biological analyte target [6,7]. Biosensors are one of the most widely studied topics due to their contributions to development of innovative medicines, which could be applied as adapted drugs or highly sensitive detectors of disease markers [8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Boron Clusters in Biomedical Applications: A Theoretical Viewpoint

Shakerzadeh¹,

Tahmasebi²,

Duong³

et al. 2022

Characteristics and Applications of Boron

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In this chapter, we presented an analysis of the recent advances in the applications of boron clusters in biomedical fields such as the development of biosensors and drug delivery systems on the basis of quantum chemical calculations. Biosensors play an essential role in many sectors, e.g., law enforcement agencies for sensing illicit drugs, medical communities for detecting overdosed medications from human and animal bodies, etc. The drug delivery systems have theoretically been proposed for many years and subsequently implemented by experiments to deliver the drug to the targeted sites by reducing the harmful side effects significantly. Boron clusters form a rich and colorful family of atomic clusters due to their unconventional structures and bonding phenomena. Boron clusters and their complexes have various biological activities such as the drug delivery, imaging for diagnosis, treatment of cancer, and probe of protein-biomolecular interactions. For all of these reactivities, the interaction mechanisms and the corresponding energetics between biomaterials and boron clusters are of essential importance as a basic step in the understanding, and thereby design of relevant materials. During the past few years, attempts have been made to probe the nature of these interactions using quantum chemical calculations mainly with density functional theory (DFT) methods. This chapter provides a summary of the theoretical viewpoint on this issue.

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Biosensor-based assay of exosome biomarker for early diagnosis of cancer

Cited by 16 publications

References 148 publications

Schwann cell‐derived exosomes promote lung cancer progression via miRNA‐21‐5p

Schwann cell‐derived exosomes promote lung cancer progression via miRNA‐21‐5p

Hypoxia-Induced miR-210 Promotes Endothelial Cell Permeability and Angiogenesis via Exosomes in Pancreatic Ductal Adenocarcinoma

Boron Clusters in Biomedical Applications: A Theoretical Viewpoint

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