Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research

Tao, Dingyin; Zhang, Lihua; Shan, Yichu; Liang, Zhen; Zhang, Yukui

doi:10.1007/s00216-010-3946-7

Cited by 38 publications

(21 citation statements)

References 90 publications

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“…Due to the small sample volume needed, high sensitivity (resulting from the reduced inner diameter of the columns and flow rate), and easy coupling with MS, it is widely applied in proteomic research [27][28][29][30]. Another important advantage, compared to conventional HPLC, is the reduced solvent consumption [31].…”

Section: Introductionmentioning

confidence: 99%

Nano-LC-MS/MS for the quantitation of prostanoids in immune cells

et al. 2014

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Prostanoids, derivatives of arachidonic acid, are involved in inflammation and immune reactions. To understand the role of prostanoids produced by diverse immune cells, a highly sensitive quantitation method for prostaglandin E2 (PGE2), prostaglandin D2 (PGD2), 6-keto prostaglandin F1α (6-keto PGF1α), prostaglandin F2α (PGF2α), and thromboxane B2 (TXB2) by means of nano-liquid chromatography-tandem mass spectrometry has been developed. It was validated according to the guidelines of the Food and Drug Administration (FDA) in terms of linearity, precision, accuracy, recovery, stability, and lower limit of quantitation (LLOQ). The LLOQ were 25 pg/mL in the injected solution (75 fg on column (o.c.)) for PGE2 and PGD2 and 37.5 pg/mL (112.5 fg on column) for 6-keto PGF1α, PGF2α, and TXB2, respectively. It was successfully applied to murine mast cells isolated from paws after zymosan injection and to CD4(+) and CD8(+) T lymphocytes from blood of sensitized versus non-sensitized mice in context of a delayed type hypersensitivity model. About 5,000 (T cells) to 40,000 (mast cells) cells were sufficient for quantitation. In the mast cells, the production of PGE2 increased at a significantly higher extent than the synthesis of the other prostanoids. The T lymphocytes did not show any difference in prostanoid production, no matter whether they were obtained from sensitized mice or non-sensitized mice.

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Section: Introductionmentioning

confidence: 99%

Nano-LC-MS/MS for the quantitation of prostanoids in immune cells

et al. 2014

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“…A recent trend in HPLC is the miniaturization of the chromatographic system, which results in decreased consumption of both the sample and the mobile phase bringing significant economical and ecological benefits [1, 2]. In miniaturized analytical systems, not only concentration but especially the mass detection limits may improve significantly [3, 4].…”

Section: Introductionmentioning

confidence: 99%

Electrochemically Pretreated Carbon Microfiber Electrodes as Sensitive HPLC-EC Detectors

Bartošová

Riman

Jakubec

et al. 2012

The Scientific World Journal

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The paper focuses on the analysis and detection of electroactive compounds using high-performance liquid chromatography (HPLC) combined with electrochemical detection (EC). The fabrication and utilization of electrochemically treated carbon fiber microelectrodes (CFMs) as highly sensitive amperometric detectors in HPLC are described. The applied pretreatment procedure is beneficial for analytical characteristics of the sensor as demonstrated by analysis of the model set of phenolic acids. The combination of CFM with separation power of HPLC technique allows for improved detection limits due to unique electrochemical properties of carbon fibers. The CFM proved to be a promising tool for amperometric detection in liquid chromatography.

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“…Agilent's HPLC-Chip Cube system (16,24) is limited to the separation power of a single dimension of RPLC. We married a SCX column (as the first dimensional HPLC column) with the Chip Cube system (second dimensional column), to enable sample loading prior to Chip-HPLC separation (Fig.…”

Section: Evaluation Of the Online 2d Scx-polaris Reversed Phase (Rp) mentioning

confidence: 99%

The Acute Transcriptomic and Proteomic Response of HC-04 Hepatoma Cells to Hepatocyte Growth Factor and its Implications for Plasmodium falciparum Sporozoite Invasion

Tao

King

Tweedell

et al. 2014

Molecular & Cellular Proteomics

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The routine study of human malaria liver-stage biology in vitro is hampered by low infection efficiency of human hepatocellular carcinoma (HCC) lines (<0.1%), poor understanding of steady-state HCC biology, and lack of appropriate tools for trace sample analysis. HC-04 is the only HCC that supports complete development of human malaria parasites. We hypothesized that HCCs are in various intermediate stages of the epithelial-mesenchymal transition (EMT) and HC-04s retain epithelial characteristics that permit infection. We developed a facile analytical approach to test this hypothesis viz. the HC-04 response to hepatocyte growth factor (HGF). We used online twodimensional liquid chromatography tandem mass spectrometry (2D-LC-MS/MS) to quantify protein expression profiles in HC-04 pre-/post-HGF treatment and validated these results by RT-qPCR and microscopy. We successfully increased protein identification efficiency over offline-2D methods by 12-fold, using less sample material, allowing robust protein quantification. We observed expected up-regulation and down-regulation of EMT protein markers in response to HGF, but also unexpected cellular responses. We also observed that HC-04 is generally more susceptible to HGF-mediated signaling than what was observed for HepG2, a widely used, but poor ma- The liver is the target tissue for several microbial pathogens (1). The malaria parasite, Plasmodium, infects the liver as the first stage of infection of a vertebrate host. Once inside hepatocytes, Plasmodium parasites undergo schizogony, developing from invasive sporozoites into hepatic schizonts containing merozoites, which subsequently infect blood cells. The development of asymptomatic pre-erythrocytic liver-stage (LS) 1 parasites of the most virulent human malaria parasite, P. falciparum remains poorly studied because of the inherent difficulty of developing in vitro LS models. A clearer understanding of LS biology may lead to the development of interventions that would result in the complete abrogation of disease. Primary hepatocytes and human hepatocellular carcinoma (HCC) cell lines are widespread models for understanding liver biology (2), chronic diseases and microbial pathogenesis (3). Although only a few Plasmodium sporozoites are required for infecting a human (4, 5), infecting hepatocyte cell lines with P. falciparum remains difficult. The use of From the ‡W. Harry Feinstone

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Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research

Cited by 38 publications

References 90 publications

Nano-LC-MS/MS for the quantitation of prostanoids in immune cells

Nano-LC-MS/MS for the quantitation of prostanoids in immune cells

Electrochemically Pretreated Carbon Microfiber Electrodes as Sensitive HPLC-EC Detectors

The Acute Transcriptomic and Proteomic Response of HC-04 Hepatoma Cells to Hepatocyte Growth Factor and its Implications for Plasmodium falciparum Sporozoite Invasion

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