Recent advances in mesoporous materials for sample preparation in proteomics research

Yao, Jizong; Sun, Nianrong; Deng, Chunhui

doi:10.1016/j.trac.2017.11.016

Cited by 54 publications

(17 citation statements)

References 159 publications

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“…On the other hand, hemoglobin subunit alpha and hemoglobin fetal subunit beta found in trace concentrations in serum, however, was the major component of the corona of all particles. Therefore, NPs with different surface chemistry can be used to enrich certain proteins for proteomic research (Wang et al, 2018;Yao et al, 2018).…”

Section: Identification Of Proteins Binding To Nps With Different Hydmentioning

confidence: 99%

Regulating Protein Corona Formation and Dynamic Protein Exchange by Controlling Nanoparticle Hydrophobicity

Zhao

Guo

et al. 2020

Front. Bioeng. Biotechnol.

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Physiochemical properties of engineered nanoparticles (NPs) play a vital role in nanobio interactions, which are critical for nanotoxicity and nanomedicine research. To understand the effects of NP hydrophobicity on the formation of the protein corona, we synthesized four gold NPs with a continuous change in hydrophobicity ranging from −2.6 to 2.4. Hydrophobic NPs adsorbed 2.1-fold proteins compared to hydrophilic ones. Proteins with small molecular weights (<50 kDa) and negatively charge (PI < 7) constituted the majority of the protein corona, especially for hydrophobic NPs. Moreover, proteins preferred binding to hydrophilic NPs (vitronectin and antithrombin III), hydrophobic NPs (serum albumin and hemoglobin fetal subunit beta), and medium hydrophobic NPs (talin 1 and prothrombin) were identified. Besides, proteins such as apolipoprotein bound to all NPs, did not show surface preference. We also found that there was a dynamic exchange between hard protein corona and solution proteins. Because of such dynamic exchanges, protein-bound NPs could expose their surface in biological systems. Hydrophilic NPs exhibited higher protein exchange rate than hydrophobic NPs. Above understandings have improved our capabilities to modulate protein corona formation by controlling surface chemistry of NPs. These will also help modulate nanotoxicity and develop better nanomedcines.

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Section: Identification Of Proteins Binding To Nps With Different Hydmentioning

confidence: 99%

Regulating Protein Corona Formation and Dynamic Protein Exchange by Controlling Nanoparticle Hydrophobicity

Zhao

Guo

et al. 2020

Front. Bioeng. Biotechnol.

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“…Most importantly, silica can be chemically functionalized that enables different approaches for covalent binding of a given enzyme. [11][12][13][14][15][16][17] Moreover, among various structures of MPS supports, hollow mesoporous silica sphere (HMS) is an extremely interesting one due to the large inner void space and relatively short diffusion paths. [18][19][20][21][22][23] So far, HMS has been used for immobilizing many enzymes, Snoch et al immobilized ethylbenzene dehydrogenase on HMS, and the immobilized enzymes were able to operate for about 24 days under anaerobic conditions.…”

Section: Introductionmentioning

confidence: 99%

Covalent Immobilization of Candida antarctica Lipase B on Functionalized Hollow Mesoporous Silica Nanoparticles

Lijuan

Xiao

et al. 2021

ChemistrySelect

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Candida antarctica lipase B (CALB) produced by recombinant Pichia pastoris was covalently immobilized to a carboxylfunctionalized hollow mesoporous silica sphere (HMS) synthesized by sol-gel precipitation. The HMS was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffractometer (XRD), and Brunauer-Emmett-Teller (BET). The immobilized CALB was characterized through Fourier transform infrared spectra (FT-IR) and elemental analysis to verify the binding of enzyme. The free and immobilized CALB were tested in tributyrin hydrolysis. Results revealed that CALB loading capacity of HMS-COOH was about 28.6 mg/g support and the corresponding enzyme activity was up to about 2700 U/g support at optimal pH and temperature, which was 6.5 and 30°C respectively. Moreover, compared with free CALB, the immobilized CALB presented more resistant towards the reaction pH and temperature changes. Furthermore, the immobilized CALB retained 60 % residual activity after eight batches of recycling and exhibited better thermal stability and storage stability, which was promising in the potential industrial applications.

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“…Porous carbon-based materials feature an ultrahigh thermal and chemical stability, while difficulties are met in their surface functionalization [ 18 – 21 ]. Mesoporous silica materials offer more flexibility for pore sizes (from a few up to dozens of nanometers) [ 22 ] and they have better chemical and thermal stability than MOFs. Further, it is easier to functionalize their mesoporous inner walls compared to those of carbon materials [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Mesoporous silica materials offer more flexibility for pore sizes (from a few up to dozens of nanometers) [ 22 ] and they have better chemical and thermal stability than MOFs. Further, it is easier to functionalize their mesoporous inner walls compared to those of carbon materials [ 22 ]. However, the most promising characteristic of mesoporous silica is its natural acidic surface that predicts a high affinity to basic amines.…”

Section: Introductionmentioning

confidence: 99%

Selective extraction of aliphatic amines by functionalized mesoporous silica-coated solid phase microextraction Arrow

et al. 2019

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Mesoporous silica-coated solid phase microextraction (SPME) Arrow systems were developed for capturing of low-molecular-weight aliphatic amines (LMWAAs) from complicated sample matrices. Specifically, silicas of type MCM-41, SBA-15 and KIT-6 were chosen as substrates to afford size-exclusion selectivity. They possess ordered multidimensional pore-channel structures and mesopore sizes between 3.8 and 8.2 nm. Their surface acidity was enhanced by grafting them with a layer of titanium hydrogenphosphate (-TP). This enhanced the chemical selectivity for basic LMWAAs. The siliceous coatings increased the extraction of ethylamine, diethylamine (DEA) and triethylamine (TEA) by factors of 18.6–102.5, 4.8–10.8 and 2.6–4.0, respectively, when compared to the commercial SPME Arrow with polydimethylsiloxane/divinylbenzene coating. Among them, the MCM-41 and MCM-41-TP coated SPME Arrows demonstrated exceptional selectivity towards LMWAAs that were quantified by gas chromatography-mass spectrometry (GC-MS). The total peak area ratios of LMWAAs/ten competing compounds were 25.4 and 36.3, respectively. The extraction equilibrium was reached within 20–30 min. The MCM-41 and MCM-41-TP derived SPME Arrows gave very similar results (18.4 ± 2.1–376 ± 12 ng g −1 to DEA and TEA) when applied to urban mushroom samples. SPME Arrow with MCM-41 coatings followed by GC-MS was applied also to the analysis of atmospheric air and urine samples resulting in high selectivity due to the size and mesoporous structure of the functionalized silica, and its chemical interactions with the LMWAAs. Graphical abstract Scheme of synthesis of the MCM-41 silicas, and the preparation of solid phase microextraction Arrow coatings. They were employed for selective capturing of aliphatic amines from complex sample matrices, followed by gas chromatography-mass spectrometry. Electronic supplementary material The online version of this article (10.1007/s00604-019-3523-5) contains supplementary material, which is available to authorized users.

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Recent advances in mesoporous materials for sample preparation in proteomics research

Cited by 54 publications

References 159 publications

Regulating Protein Corona Formation and Dynamic Protein Exchange by Controlling Nanoparticle Hydrophobicity

Regulating Protein Corona Formation and Dynamic Protein Exchange by Controlling Nanoparticle Hydrophobicity

Covalent Immobilization of Candida antarctica Lipase B on Functionalized Hollow Mesoporous Silica Nanoparticles

Selective extraction of aliphatic amines by functionalized mesoporous silica-coated solid phase microextraction Arrow

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