Recent Advances in Liver Engineering With Decellularized Scaffold

Abstract

Liver transplantation is currently the only effective treatment for patients with end-stage liver disease; however, donor liver scarcity is a notable concern. As a result, extensive endeavors have been made to diversify the source of donor livers. For example, the use of a decellularized scaffold in liver engineering has gained considerable attention in recent years. The decellularized scaffold preserves the original orchestral structure and bioactive chemicals of the liver, and has the potential to create a de novo liver that is fit for transplantation after recellularization. The structure of the liver and hepatic extracellular matrix, decellularization, recellularization, and recent developments are discussed in this review. Additionally, the criteria for assessment and major obstacles in using a decellularized scaffold are covered in detail.

Cirrhotic Liver Sustains In Situ Regeneration of Acellular Liver Scaffolds after Transplantation into G-CSF-Treated Animals

“…Decellularization is an efficient method for generating cell-free tissues [ 15 ]. In this study, we applied continuous perfusion of a chemical detergent to eliminate the cellular components of normal rat livers.…”

Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine

“…It uses physical stresses to enhance agent effects on intended tissues. However, its efficacy is relatively low for removing the immunogenic cellular content of large organs or dense tissues [68]. On the other hand, perfusion exploits a pump to induce an artificial circulation of chemical and biological agents through the whole organ vasculature.…”

Biomedical Application of Decellularized Scaffolds

“…Therefore, there is an urgent need to develop alternative therapies for these conditions. One promising alternative is to produce a natural biofiber by decellularizing the liver. , …”

“…One promising alternative is to produce a natural biofiber by decellularizing the liver. 169,170 Naeem et al developed a method for in situ recellularization of rat liver using sodium lauryl ether sulfate (SLES). It has been found through research that the decellularized liver prepared by this method can be appropriately vascularized and regenerated after transplantation.…”